Glycine Betaine-Mediated Root Priming Improves Water Stress Tolerance in Wheat (Triticum aestivum L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Growth and Biomass Accumulation
2.2. Root Characteristics
2.3. Root Antioxidant Enzymes Extractions and Assays
2.4. Quantification of the Malondialdehyde (MDA) and Hydrogen Peroxide (H2O2) Content
2.5. Quantification of Proline and Total Soluble Sugars
2.6. Quantification of ABA, SA and JA and in Wheat Roots
2.7. RNA Isolation, cDNA Synthesis, and Quantitative PCR Analysis
2.8. Statistical Analysis
3. Results
3.1. Root Characteristics
3.2. Wheat Seedling Fresh Weight and Dry Matter Accumulation
3.2.1. Antioxidant Enzymes Activity
3.2.2. MDA and H2O2 Accumulation
3.3. Osmolytes Accumulation
3.4. Hormones
3.5. Changes in Expression of Genes Involved in ABA Metabolism
3.6. Changes in Expression of Genes Involved in ABA Downstream Signaling
3.7. Changes in Expression Patterns of Different Transcriptional Factors
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Ahmed, N.; Zhu, M.; Li, Q.; Wang, X.; Wan, J.; Zhang, Y. Glycine Betaine-Mediated Root Priming Improves Water Stress Tolerance in Wheat (Triticum aestivum L.). Agriculture 2021, 11, 1127. https://doi.org/10.3390/agriculture11111127
Ahmed N, Zhu M, Li Q, Wang X, Wan J, Zhang Y. Glycine Betaine-Mediated Root Priming Improves Water Stress Tolerance in Wheat (Triticum aestivum L.). Agriculture. 2021; 11(11):1127. https://doi.org/10.3390/agriculture11111127
Chicago/Turabian StyleAhmed, Nazir, Mingyuan Zhu, Qiuxia Li, Xilei Wang, Jiachi Wan, and Yushi Zhang. 2021. "Glycine Betaine-Mediated Root Priming Improves Water Stress Tolerance in Wheat (Triticum aestivum L.)" Agriculture 11, no. 11: 1127. https://doi.org/10.3390/agriculture11111127