Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm
Abstract
:1. Introduction
2. Material and Methods
2.1. Direct Contact Test
2.2. Multispecies Biofilm Inhibition Test
2.2.1. Specimen Preparation
2.2.2. Multispecies Biofilm Growth
2.2.3. Determination of Quantitative Viable Counts of the Biofilms after 14 Days
2.2.4. Confocal Laser Scanning Microscopy (CLSM) Analysis of the Multispecies Biofilm
2.3. Determination of pH
2.4. Statistical Analysis
3. Results
3.1. Direct Contact Test
3.1.1. Effect of Sealers on Enterococcus faecalis
3.1.2. Effect of Sealers on Actinomyces radicidentis
3.1.3. Effect of Sealers on Propionibacterium acnes
3.1.4. Effect of Sealers on Staphylococcus epidermidis
3.1.5. Effect of Sealers on Streptococcus mitis/oralis
3.2. Determination of Quantitative Viable Counts of the Biofilms after 14 Days
3.3. Confocal Laser Scanning Microscopy (CLSM) Analysis of the Effect of Endodontic Sealers on the Inhibition of Multispecies Biofilm
3.4. pH Values of the Endodontic Sealers
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sealer | Type | Availability | Composition |
---|---|---|---|
TotalFill BC | Calcium silicate | Preloaded syringe | 35.0−45.0% Zirconium Oxide 20.0−35.0% Tricalcium silicate 7.0−15.0% Dicalcium silicate 1.0−4.0% Calcium hydroxide |
BioRoot RCS | Calcium silicate | Hand-mix presentation | (a) Powder: Tricalcium silicate, zirconium oxide, Povidone (b) Liquid: Aqueous solution of calcium chloride and polycarboxylate |
AH Plus | Epoxy resin | Mixing syringe | (a) Epoxide paste: Diepoxide, Calcium tungstate, Zirconium Oxide, Aerosil, Pigment (b) Amine Paste:1-adamantane-amine, N,N′-dibenyl-5-oxa-nonandiamine-1-9,TCD-Diamine, Calcium tungstate, Aerosil, Silicone oil |
Tubli-seal | Zinc Oxide Eugenol | Mixing syringe | (a) Accelerator: 10−50% eugenol, 20% 5,5′-diisopropyl-2,2′-dimethylbiphenyl-4,4′-diyl dihypoiodite <2.5% zinc acetate dihydrate (b)Base: 50−80% zinc oxide,1−30%, Petroleum, barium sulphate, Starch |
Sealer | Setting Times |
---|---|
TotalFill BC | 4–10 h |
BioRoot RCS | 4 h |
AH Plus | 24 h |
Tubli-seal | 2 h |
Bacteria | Sealers | T1, Mean ± SE | T2, Mean ± SE | T3, Mean ± SE |
---|---|---|---|---|
EF | BioRoot RCS | 7.36 (0.32) | 6.45 (0.09) * | 2.74 (0.21) * ***** |
AH Plus | 5.98 (0.68) * ** **** | 3.81 (0.77) * | 3.36 (0.36) * | |
TotalFill BC | 7.71 (0.02) | 6.97 (0.1) | 3.12 (0.15) * | |
Tubli-seal | 6.40 (0.58) * | 4.91 (0.44) * | 4.47 (0.23) * | |
AR | BioRoot RCS | 5.42 (0.38) * | 4.21 (0.24) * ***** | 3.96 (0.09) * |
AH Plus | 6.39 (0.02) * | 3.77 (0.32) * ***** | 3.31 (0.71) * | |
TotalFill BC | 6.21 (0.1) * | 4.77 (0.05) * ***** | 4.54 (0.12) * | |
Tubli-seal | 6.65 (0.03) * | 6.06 (0.04) * | 5.22 (0.19) * | |
PA | BioRoot RCS | 5.26 (0.28) * *** **** | 4.33 (0.21) * | 2.72 (0.11) * **** |
AH Plus | 6.63 (0.06) * **** | 4.77 (0.12) * | 4.40 (1.21) * | |
TotalFill BC | 7.75 (0.09) | 5.80 (0.70) * | 4.53 (0.09) * | |
Tubli-seal | 4.69 (0.29) * *** **** | 3.56 (1.01) * | 3.14 (0.49) * | |
SE | BioRoot RCS | 5.27 (0.18) * ***** | 3.54 (0.60) * **** ***** | 2.44 (0.23) * **** |
AH Plus | 6.23 (0.16) * ***** | 2.97 (0.12) * **** ***** | 2.13 (0.07) * **** | |
TotalFill BC | 6.13 (0.13) * ***** | 5.22 (0.11) * | 5.50 (0.10) * | |
Tubli-seal | 7.42 (0.13) | 6.29 (0.32) * | 3.79 (0.43) * **** | |
Sm/o | BioRoot RCS | 4.73 (0.25) * **** | 4.03 (0.28) * **** | 3.01 (0.08) * *** **** |
AH Plus | 5.11 (0.57) * | 4.39 (0.77) * | 3.84 (0.22) * | |
TotalFill BC | 5.77 (0.18) * | 5.39 (0.03) * | 4.56 (0.25) * | |
Tubli-seal | 4.30 (0.47) * *** **** | 3.53 (0.14) * **** | 3.34 (0.05) * *** **** |
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Bose, R.; Ioannidis, K.; Foschi, F.; Bakhsh, A.; Kelly, R.D.; Deb, S.; Mannocci, F.; Niazi, S.A. Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm. J. Clin. Med. 2020, 9, 2722. https://doi.org/10.3390/jcm9092722
Bose R, Ioannidis K, Foschi F, Bakhsh A, Kelly RD, Deb S, Mannocci F, Niazi SA. Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm. Journal of Clinical Medicine. 2020; 9(9):2722. https://doi.org/10.3390/jcm9092722
Chicago/Turabian StyleBose, Rahul, Konstantinos Ioannidis, Federico Foschi, Abdulaziz Bakhsh, Robert D. Kelly, Sanjukta Deb, Francesco Mannocci, and Sadia Ambreen Niazi. 2020. "Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm" Journal of Clinical Medicine 9, no. 9: 2722. https://doi.org/10.3390/jcm9092722