Comparative Analysis of Swine Antibody Responses following Vaccination with Live-Attenuated and Killed African Swine Fever Virus Vaccines
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells, Viruses, and Control Antisera
2.2. Experimental Live-Attenuated Virus and Inactivated Virus Vaccine
2.3. Animal Experiment
2.4. Realtime PCR to Measure Viral Genomic DNA
2.5. ELISA to Measure Antibody Responses
2.6. Plasmid Construction and Protein Expression
2.7. Luciferase-Immunoprecipitation System
2.8. Statistical Analysis
3. Results
3.1. Virological and Serological Response after Vaccination
3.2. Live-Attenuated Virus but Not Killed Virus Vaccine Conferred Protection against Lethal ASFV Challenge
3.3. Differential Antibody Responses between Pigs Vaccinated with a Live-Attenuated Virus Vaccine and Those Vaccinated with a Killed Virus Vaccine
3.4. The Intensity of Antibody Binding to p12, p14, p15, p32, and pD205R Exhibited a Negative Correlation with the Viral Loads after Challenge Infection
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Luong, H.Q.; Lai, H.T.L.; Truong, L.Q.; Nguyen, T.N.; Vu, H.D.; Nguyen, H.T.; Nguyen, L.T.; Pham, T.H.; McVey, D.S.; Vu, H.L.X. Comparative Analysis of Swine Antibody Responses following Vaccination with Live-Attenuated and Killed African Swine Fever Virus Vaccines. Vaccines 2023, 11, 1687. https://doi.org/10.3390/vaccines11111687
Luong HQ, Lai HTL, Truong LQ, Nguyen TN, Vu HD, Nguyen HT, Nguyen LT, Pham TH, McVey DS, Vu HLX. Comparative Analysis of Swine Antibody Responses following Vaccination with Live-Attenuated and Killed African Swine Fever Virus Vaccines. Vaccines. 2023; 11(11):1687. https://doi.org/10.3390/vaccines11111687
Chicago/Turabian StyleLuong, Hung Q., Huong T. L. Lai, Lam Q. Truong, The N. Nguyen, Hanh D. Vu, Hoa T. Nguyen, Lan T. Nguyen, Trang H. Pham, D. Scott McVey, and Hiep L. X. Vu. 2023. "Comparative Analysis of Swine Antibody Responses following Vaccination with Live-Attenuated and Killed African Swine Fever Virus Vaccines" Vaccines 11, no. 11: 1687. https://doi.org/10.3390/vaccines11111687