Inhibition of Heme Oxygenase Antioxidant Activity Exacerbates Hepatic Steatosis and Fibrosis In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Oil Red O Staining
2.3. ROS Measurement
2.4. Sircol Collagen Assay
2.5. RNA Extraction and qRT-PCR
2.6. Statistical Analyses
3. Results
3.1. Effect of HO Inhibition on Hepatic Fatty Storage
3.2. Effect of HO Regulation on Lipid Metabolism Pathway
3.3. Hepatic Fibrosis In Vitro Model
3.4. Effect of HO Inhibition on ROS Generation and Soluble Collagen Release
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Raffaele, M.; Carota, G.; Sferrazzo, G.; Licari, M.; Barbagallo, I.; Sorrenti, V.; Signorelli, S.S.; Vanella, L. Inhibition of Heme Oxygenase Antioxidant Activity Exacerbates Hepatic Steatosis and Fibrosis In Vitro. Antioxidants 2019, 8, 277. https://doi.org/10.3390/antiox8080277
Raffaele M, Carota G, Sferrazzo G, Licari M, Barbagallo I, Sorrenti V, Signorelli SS, Vanella L. Inhibition of Heme Oxygenase Antioxidant Activity Exacerbates Hepatic Steatosis and Fibrosis In Vitro. Antioxidants. 2019; 8(8):277. https://doi.org/10.3390/antiox8080277
Chicago/Turabian StyleRaffaele, Marco, Giuseppe Carota, Giuseppe Sferrazzo, Maria Licari, Ignazio Barbagallo, Valeria Sorrenti, Salvatore S. Signorelli, and Luca Vanella. 2019. "Inhibition of Heme Oxygenase Antioxidant Activity Exacerbates Hepatic Steatosis and Fibrosis In Vitro" Antioxidants 8, no. 8: 277. https://doi.org/10.3390/antiox8080277