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Applied Sciences
Volume 13
Issue 4
10.3390/app13042477
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Article
Peer-Review Record

UHPLC-QTOF/MS Untargeted Lipidomics and Caffeine Carry-Over in Milk of Goats under Spent Coffee Ground Enriched Diet

Appl. Sci. 2023, 13(4), 2477; https://doi.org/10.3390/app13042477
by Mattia Casula 1, Paola Scano 1,*, Cristina Manis 1, Giulia Tolle 1, Anna Nudda 2, Silvia Carta 2, Giuseppe Pulina 2 and Pierluigi Caboni 1
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Reviewer 3:
Essam Ezzeldin
Appl. Sci. 2023, 13(4), 2477; https://doi.org/10.3390/app13042477
Submission received: 23 January 2023 / Revised: 10 February 2023 / Accepted: 13 February 2023 / Published: 14 February 2023
(This article belongs to the Special Issue Advance in Processing and Quality Control of Dairy Products)

Round 1

Reviewer 1 Report

The manuscript submitted for review is interesting and novel, however, it is necessary to address the questions and observations of the pdf file.

Comments for author File: Comments.pdf

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

This manuscript studies the effect of adding agro-industrial by-products spent coffee grounds to the diet of dairy goats on the quality of goat milk, which provides a new way for the waste utilization of spent coffee grounds, which has some novelty.

1. In 2.1, the addition amount of spent coffee grounds is only set to low-dose (50g/d) and high-dose (100g/d), why not set middle-dose (75g/d)?

2. In the results and discussion, the author mainly discusses the effect of adding spent coffee grounds to dairy goat diet on Caffeine levels and lipid metabolism in goat milk. The influence of physical and chemical properties such as protein and pH? These are also the basic characteristics of goat milk. If there are relevant data, it is recommended to supplement this information

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 3 Report

The purpose was develop for study UHPLC-QTOF/MS Untargeted lipidomics and caffeine carry-over in milk of goats 2 under spent coffee ground enriched diet. The idea of the study is interesting. However, the following point should be taken into consideration:

-          In 2.2. Sample preparation and UHPLC-QTOF/MS analysis (page 2): References or the detail of the methods.

-          In 2.2. Sample preparation and UHPLC-QTOF/MS analysis (page 3): Please define and compare between Chromatographic elution and Chromatographic separation.

-          Results should be supported by figure and scan

-           

 

.

 

 

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Round 2

Reviewer 3 Report

Authors have responded to the comments

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