Preliminary Evaluation of the Use of Thermally-Dried Immobilized Kefir Cells in Low Alcohol Winemaking
Abstract
:1. Introduction
2. Materials and Methods
2.1. Immobilization of Kefir Culture and Fermentation Medium
2.2. Thermal Drying and Storage of Kefir Cells
2.3. Determination of Cell Viability of Thermally-Dried Cells
2.4. Fermentations
2.5. Chemical Analyses
2.6. Quality and Kinetic Parameters Determination
- Ethanol productivity: g of ethanol produced per day per liter of liquid volume of bioreactor.
- Conversion: (Initial sugar conc.—Residual sugar conc.)/Initial sugar conc. * 100.
- Ethanol production yield: g of ethanol produced per g of sugars utilized.
- Malic acid conversion: (Initial malic acid conc.—Residual malic acid conc.)/Initial malic acid conc. * 100.
2.7. Preliminary Sensory Analysis
2.8. Statistical Analysis
3. Results and Discussion
3.1. Cell Viability of Thermally-Dried Immobilized Kefir Cells
3.2. Fermentations
3.2.1. Fermentation Efficiency
3.2.2. Fermentation Stability
3.3. Volatiles
3.3.1. Major Volatiles
3.3.2. Minor Volatiles
3.4. Preliminary Sensory Evaluation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Free Cells | Immobilized Cells on Apple Pieces | Immobilized Cells on DCM | Immobilized Cells on Grape Skins | |
---|---|---|---|---|
Yeasts/Molds | 5.55 ± 0.05 | 6.95 ± 0.09 | 7.07 ± 0.05 | 7.35 ± 0.05 |
Lactobacilli | 3.12 ± 0.07 | 4.25 ± 0.06 | 5.36 ± 0.05 | 4.60 ± 0.05 |
Lactococci | 3.02 ± 0.21 | 4.15 ± 0.15 | 5.49 ± 0.05 | 4.65 ± 0.08 |
Nature of Thermally-Dried Kefir Culture | Fermentation Temperature (°C) | Repeated Batches | Fermentation Time (h) | Ethanol Concentration (g/L) | Glycerol (g/L) | Residual Sugars (g/L) | Ethanol Productivity (g/L d) | Ethanol Production Yield (g/g) | Conversion (%) | Malic Acid * (g/L) | Lactic Acid (g/L) | Malic Acid Conversion (%) | Acetic Acid (g/L) | Citric Acid (g/L) | Total Acidity (g Tartaric/L) | Volatile Acidity (g Acetic/L) | pH |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Free cells | 30 | 1–3 | 168–216 | 8.6–9.2 | 3.9–4.5 | 1.0–2.2 | 8.0–10.1 | 0.40–0.43 | 98.7–99.2 | 1.3–1.5 | 0.1–0.2 | 37.2–41.3 | 0.5–0.9 | 0.6–0.9 | 3.4–3.6 | 0.43–0.51 | 4.1 |
20 | 4–6 | 312–360 | 8.6–10.0 | 4.4–5.9 | 0.7–3.7 | 4.5–6.2 | 0.41–0.47 | 97.8–99.6 | 1.4–1.7 | 0.1 | 30.3–38.2 | 0.5–0.9 | 0.3–0.9 | 3.5–3.7 | 0.42–0.46 | 4.1 | |
5 | 7–8 | 1404–1476 | 2.9–3.1 | 2.9–3.1 | 68.6–73.2 | 0.4 | 0.23–0.25 | 56.9–59.6 | 1.4–1.5 | 0.1 | 33.8–39.1 | 0.4–0.5 | 0.9–1.0 | 3.6 | 0.39–0.40 | 4.0 | |
Immobilized cells on apple pieces | 30 | 1–3 | 120–144 | 7.3–8.4 | 4.4–4.8 | 0.9–1.1 | 9.7–13.3 | 0.34–0.39 | 99.3–99.5 | 1.7–1.9 | 0.1–0.2 | 17.7–25.0 | 0.5–1.1 | 0.5–1.0 | 5.1–5.2 | 0.44–0.47 | 3.7–3.8 |
20 | 4–6 | 276–312 | 8.7–9.3 | 5.6–6.8 | 1.1–1.3 | 5.5–6.0 | 0.41–0.44 | 99.2–99.4 | 1.9–2.1 | 0.3–0.4 | 13.0–17.4 | 0.4–0.7 | 0.6–0.9 | 5.1–5.2 | 0.41–0.48 | 3.7–3.8 | |
5 | 7–8 | 1536–1584 | 6.7–7.1 | 5.0–5.3 | 21.3–25.1 | 0.8–0.9 | 0.37–0.38 | 85.2–87.4 | 1.6–1.8 | 0.2 | 23.7–30.4 | 0.5–0.6 | 0.8–0.9 | 5.1 | 0.38 | 3.7–3.8 | |
Immobilized cells on DCM | 30 | 1–3 | 144–168 | 7.2–7.9 | 4.3–5.2 | 0.9–1.1 | 8.1–10.4 | 0.34–0.37 | 99.3–99.5 | 1.3–1.8 | 0.2 | 21.1–41.4 | 0.6–1.4 | 0.5–0.6 | 5.3–5.4 | 0.33–0.41 | 3.8–3.9 |
20 | 4–6 | 264–288 | 8.6–10.0 | 4.4–7.2 | 1.4–3.7 | 5.6–7.0 | 0.41–0.47 | 97.8–99.2 | 1.4–1.9 | 0.1–0.3 | 20.8–37.0 | 0.5–0.8 | 0.4–1.0 | 5.3–5.4 | 0.35–0.40 | 3.8 | |
5 | 7–8 | 1500–1560 | 4.4–5.0 | 3.6–4.0 | 55.0–62.2 | 0.6 | 0.32–0.34 | 63.4–67.6 | 1.8–1.9 | 0.1 | 16.2–21.7 | 0.5–0.7 | 1.0–1.1 | 5.3 | 0.32–0.35 | 3.8 | |
Immobilized cells on grape skins | 30 | 1–3 | 120–168 | 6.4–7.3 | 4.7–5.6 | 0.9–1.2 | 8.2–10.7 | 0.30–0.34 | 99.3–99.5 | 1.5–1.9 | 0.2 | 18.8–36.1 | 0.5–1.4 | 0.6–0.8 | 5.1 | 0.48–0.54 | 3.9 |
20 | 4–6 | 288–312 | 8.7–9.1 | 6.2–6.5 | 1.3–1.5 | 5.3–6.0 | 0.41–0.43 | 99.1–99.2 | 1.7–2.0 | 0.2–0.3 | 13.0–26.1 | 0.6–0.8 | 0.5–0.7 | 5.1 | 0.50–0.57 | 3.9 | |
5 | 7–8 | 1392–1440 | 6.5–7.1 | 5.1–5.3 | 25.8–30.6 | 0.9–1.0 | 0.37–0.39 | 82.0–84.8 | 1.4–1.6 | 0.2 | 30.4–39.3 | 0.7–0.8 | 0.5–0.6 | 5.1–5.2 | 0.47–0.48 | 3.8 |
Nature of Thermally-Dried Kefir Culture | Months of Storage | Storage Temperature (°C) | Fermentation Time (h) | Ethanol Concentration (g/L) | Glycerol (g/L) | Residual Sugars (g/L) | Ethanol Productivity (g/L d) | Ethanol Production Yield (g/g) | Conversion (%) | Malic Acid * (g/L) | Lactic Acid (g/L) | Malic Acid Conversion (%) | Acetic Acid (g/L) | Citric Acid (g/L) | Total Acidity (g Tartaric/L) | Volatile Acidity (g Acetic/L) | pH |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Free cells | 1 | 20 | 360 ± 51 | 8.7 ± 1.4 | 4.2 ± 0.8 | 4.3 ± 1.7 | 4.6 ± 0.1 | 0.44 ± 0.06 | 95.8 ± 2.1 | 2.0 ± 0.1 | 0.2 ± 0.1 | 13.0 ± 6.1 | 0.4 ± 0.1 | 1.3 ± 0.4 | 3.5 ± 0.2 | 0.44 ± 0.07 | 4.0 ± 0.1 |
5 | 312 ± 44 | 9.7 ± 1.0 | 4.6 ± 0.7 | 5.0 ± 1.5 | 5.9 ± 0.3 | 0.46 ± 0.04 | 96.5 ± 0.1 | 1.8 ± 0.1 | 0.2 ± 0.1 | 21.7 ± 5.5 | 0.5 ± 0.1 | 1.5 ± 0.4 | 3.5 ± 0.2 | 0.45 ± 0.07 | 4.1 ± 0.1 | ||
−18 | 312 ± 44 | 9.3 ± 1.4 | 5.6 ± 0.9 | 1.9 ± 0.6 | 5.6 ± 0.1 | 0.44 ± 0.07 | 98.7 ± 0.1 | 1.9 ± 0.1 | 0.3 ± 0.1 | 17.4 ± 5.8 | 0.5 ± 0.1 | 1.6 ± 0.5 | 3.4 ± 0.3 | 0.44 ± 0.06 | 4.0 ± 0.1 | ||
3 | 20 | 408 ± 58 | 8.9 ± 1.8 | 4.6 ± 1.4 | 1.4 ± 0.4 | 4.1 ± 0.2 | 0.42 ± 0.08 | 99.2 ± 0.2 | 1.7 ± 0.2 | 0.2 ± 0.1 | 26.1 ± 8.4 | 0.4 ± 0.1 | 0.6 ± 0.2 | 3.3 ± 0.4 | 0.44 ± 0.06 | 4.0 ± 0.1 | |
5 | 360 ± 41 | 9.9 ± 0.7 | 4.9 ± 1.0 | 1.5 ± 0.4 | 5.2 ± 0.1 | 0.46 ± 0.04 | 99.1 ± 0.3 | 1.6 ± 0.1 | 0.2 ± 0.1 | 28.6 ± 3.1 | 0.5 ± 0.1 | 0.6 ± 0.2 | 3.3 ± 0.4 | 0.50 ± 0.08 | 4.1 ± 0.1 | ||
−18 | 360 ± 41 | 10.1 ± 0.5 | 5.2 ± 0.9 | 1.5 ± 0.4 | 5.3 ± 0.3 | 0.47 ± 0.03 | 99.1 ± 0.3 | 1.6 ± 0.1 | 0.2 ± 0.1 | 31.6 ± 3.0 | 0.5 ± 0.1 | 0.9 ± 0.3 | 3.4 ± 0.4 | 0.50 ± 0.07 | 4.1 ± 0.1 | ||
6 | 20 | 408 ± 75 | 9.4 ± 1.1 | 5.0 ± 1.3 | 2.0 ± 0.6 | 4.6 ± 0.3 | 0.46 ± 0.05 | 98.8 ± 0.3 | 2.1 ± 0.1 | 0.2 ± 0.1 | 8.7 ± 3.9 | 0.4 ± 0.1 | 0.7 ± 0.2 | 3.4 ± 0.6 | 0.48 ± 0.07 | 4.1 ± 0.1 | |
5 | 408 ± 58 | 9.5 ± 1.2 | 5.2 ± 0.9 | 1.9 ± 0.6 | 4.4 ± 0.1 | 0.45 ± 0.06 | 98.9 ± 0.3 | 2.0 ± 0.1 | 0.2 ± 0.1 | 13.0 ± 4.9 | 0.4 ± 0.1 | 0.9 ± 0.3 | 3.4 ± 0.6 | 0.51 ± 0.08 | 4.0 ± 0.1 | ||
−18 | 360 ± 51 | 10.1 ± 0.4 | 5.9 ± 0.8 | 1.7 ± 0.5 | 5.5 ± 0.5 | 0.46 ± 0.02 | 99.0 ± 0.3 | 1.8 ± 0.1 | 0.4 ± 0.1 | 25.0 ± 4.2 | 0.5 ± 0.1 | 1.0 ± 0.3 | 3.5 ± 0.6 | 0.55 ± 0.09 | 4.0 ± 0.1 | ||
Immobilized cells on apple pieces | 1 | 20 | 240 ± 34 | 8.6 ± 1.8 | 6.4 ± 1.3 | 1.3 ± 0.5 | 6.8 ± 0.5 | 0.40 ± 0.08 | 99.2 ± 0.3 | 2.1 ± 0.1 | 0.4 ± 0.2 | 8.7 ± 2.6 | 0.4 ± 0.1 | 1.7 ± 0.5 | 5.1 ± 0.4 | 0.48 ± 0.08 | 3.8 ± 0.1 |
5 | 240 ± 34 | 8.8 ± 1.2 | 6.2 ± 1.2 | 1.3 ± 0.5 | 7.0 ± 0.1 | 0.41 ± 0.06 | 99.2 ± 0.3 | 2.0 ± 0.1 | 0.5 ± 0.2 | 13.0 ± 2.5 | 0.4 ± 0.1 | 1.7 ± 0.5 | 5.0 ± 0.4 | 0.45 ± 0.08 | 3.8 ± 0.1 | ||
−18 | 240 ± 31 | 9.3 ± 1.3 | 6.1 ± 1.2 | 1.6 ± 0.6 | 7.3 ± 0.1 | 0.44 ± 0.06 | 99.1 ± 0.3 | 1.8 ± 0.1 | 0.4 ± 0.2 | 21.7 ± 2.2 | 0.4 ± 0.1 | 1.6 ± 0.5 | 5.1 ± 0.4 | 0.43 ± 0.07 | 3.8 ± 0.1 | ||
3 | 20 | 264 ± 52 | 7.2 ± 2.1 | 4.7 ± 1.5 | 1.1 ± 0.4 | 5.1 ± 0.5 | 0.34 ± 0.10 | 99.4 ± 0.2 | 2.0 ± 0.1 | 0.3 ± 0.1 | 13.0 ± 2.5 | 0.2 ± 0.1 | 0.9 ± 0.3 | 4.8 ± 0.5 | 0.47 ± 0.10 | 3.8 ± 0.1 | |
5 | 240 ± 31 | 8.3 ± 1.4 | 5.8 ± 1.0 | 1.3 ± 0.5 | 6.5 ± 0.3 | 0.39 ± 0.07 | 99.2 ± 0.3 | 1.9 ± 0.1 | 0.3 ± 0.1 | 17.4 ± 2.3 | 0.4 ± 0.1 | 0.9 ± 0.3 | 5.0 ± 0.6 | 0.38 ± 0.08 | 3.8 ± 0.1 | ||
−18 | 240 ± 27 | 7.8 ± 1.2 | 5.6 ± 1.0 | 1.2 ± 0.4 | 6.1 ± 0.3 | 0.36 ± 0.06 | 99.3 ± 0.2 | 1.5 ± 0.1 | 0.2 ± 0.1 | 34.8 ± 1.8 | 0.4 ± 0.1 | 1.0 ± 0.3 | 5.0 ± 0.6 | 0.41 ± 0.09 | 3.8 ± 0.1 | ||
6 | 20 | 264 ± 56 | 7.6 ± 1.8 | 5.1 ± 1.4 | 1.2 ± 0.4 | 5.4 ± 0.2 | 0.36 ± 0.08 | 99.3 ± 0.2 | 2.2 ± 0.1 | 0.3 ± 0.1 | 4.3 ± 2.7 | 0.3 ± 0.1 | 0.7 ± 0.2 | 5.0 ± 0.4 | 0.45 ± 0.10 | 3.8 ± 0.1 | |
5 | 240 ± 34 | 7.8 ± 1.4 | 5.3 ± 0.7 | 1.2 ± 0.4 | 6.1 ± 0.2 | 0.36 ± 0.07 | 99.3 ± 0.2 | 2.0 ± 0.1 | 0.3 ± 0.1 | 13.0 ± 3.7 | 0.4 ± 0.1 | 1.0 ± 0.3 | 5.1 ± 0.4 | 0.50 ± 0.07 | 3.8 ± 0.1 | ||
−18 | 240 ± 34 | 7.4 ± 1.0 | 5.9 ± 0.8 | 1.1 ± 0.4 | 5.8 ± 0.1 | 0.35 ± 0.05 | 99.4 ± 0.2 | 1.8 ± 0.1 | 0.5 ± 0.2 | 21.7 ± 2.2 | 0.4 ± 0.1 | 0.9 ± 0.3 | 5.1 ± 0.4 | 0.48 ± 0.07 | 3.8 ± 0.1 | ||
Immobilized cells on DCM | 1 | 20 | 240 ± 32 | 8.0 ± 0.8 | 6.6 ± 0.7 | 1.4 ± 0.4 | 6.3 ± 0.2 | 0.37 ± 0.04 | 99.2 ± 0.3 | 2.0 ± 0.1 | 0.3 ± 0.1 | 13.0 ± 5.5 | 0.5 ± 0.1 | 0.3 ± 0.1 | 5.4 ± 0.6 | 0.40 ± 0.08 | 3.9 ± 0.1 |
5 | 240 ± 32 | 7.7 ± 0.8 | 6.6 ± 0.5 | 1.3 ± 0.4 | 6.1 ± 0.2 | 0.36 ± 0.03 | 99.2 ± 0.2 | 2.0 ± 0.1 | 0.4 ± 0.2 | 13.0 ± 4.3 | 0.5 ± 0.1 | 0.3 ± 0.1 | 5.4 ± 0.6 | 0.38 ± 0.05 | 3.9 ± 0.1 | ||
−18 | 240 ± 27 | 9.4 ± 0.9 | 6.6 ± 0.5 | 1.4 ± 0.4 | 7.4 ± 0.1 | 0.44 ± 0.04 | 99.2 ± 0.3 | 1.9 ± 0.1 | 0.4 ± 0.2 | 17.4 ± 3.5 | 0.7 ± 0.1 | 0.4 ± 0.1 | 5.4 ± 0.6 | 0.40 ± 0.06 | 3.8 ± 0.1 | ||
3 | 20 | 240 ± 48 | 7.1 ± 1.1 | 5.3 ± 1.1 | 1.2 ± 0.4 | 5.6 ± 0.2 | 0.33 ± 0.05 | 99.3 ± 0.2 | 2.0 ± 0.1 | 0.3 ± 0.1 | 13.0 ± 4.9 | 0.3 ± 0.1 | 0.1 ± 0.1 | 5.4 ± 0.5 | 0.40 ± 0.07 | 3.9 ± 0.1 | |
5 | 240 ± 42 | 5.6 ± 0.8 | 5.0 ± 0.7 | 0.8 ± 0.2 | 4.4 ± 0.2 | 0.26 ± 0.04 | 99.5 ± 0.1 | 2.0 ± 0.1 | 0.2 ± 0.1 | 13.0 ± 3.7 | 0.3 ± 0.1 | 0.2 ± 0.1 | 5.4 ± 0.5 | 0.38 ± 0.05 | 3.8 ± 0.1 | ||
−18 | 240 ± 34 | 6.3 ± 0.9 | 5.4 ± 0.8 | 1.0 ± 0.3 | 5.0 ± 0.1 | 0.29 ± 0.04 | 99.4 ± 0.2 | 1.9 ± 0.1 | 0.3 ± 0.1 | 17.4 ± 3.5 | 0.4 ± 0.1 | 0.1 ± 0.1 | 5.3 ± 0.4 | 0.40 ± 0.05 | 3.8 ± 0.1 | ||
6 | 20 | 264 ± 56 | 7.2 ± 1.8 | 5.4 ± 1.6 | 1.1 ± 0.3 | 5.1 ± 0.2 | 0.34 ± 0.09 | 99.4 ± 0.2 | 2.2 ± 0.1 | 0.2 ± 0.1 | 4.3 ± 2.7 | 0.3 ± 0.1 | 0.4 ± 0.1 | 5.3 ± 0.4 | 0.45 ± 0.08 | 3.8 ± 0.1 | |
5 | 240 ± 37 | 6.5 ± 1.1 | 5.4 ± 0.9 | 1.0 ± 0.3 | 5.1 ± 0.1 | 0.30 ± 0.05 | 99.4 ± 0.2 | 2.0 ± 0.1 | 0.2 ± 0.1 | 13.0 ± 2.5 | 0.3 ± 0.1 | 0.2 ± 0.1 | 5.4 ± 0.5 | 0.40 ± 0.07 | 3.8 ± 0.1 | ||
−18 | 240 ± 37 | 6.7 ± 0.9 | 5.3 ± 0.8 | 1.0 ± 0.3 | 5.3 ± 0.1 | 0.31 ± 0.04 | 99.4 ± 0.2 | 1.9 ± 0.1 | 0.4 ± 0.2 | 17.4 ± 2.3 | 0.3 ± 0.1 | 0.2 ± 0.1 | 5.4 ± 0.5 | 0.38 ± 0.05 | 3.8 ± 0.1 | ||
Immobilized cells on grape skins | 1 | 20 | 264 ± 35 | 9.2 ± 0.9 | 7.0 ± 0.8 | 1.8 ± 0.6 | 6.6 ± 0.2 | 0.43 ± 0.04 | 98.9 ± 0.3 | 1.7 ± 0.1 | 0.3 ± 0.1 | 26.1 ± 5.2 | 0.5 ± 0.1 | 0.3 ± 0.1 | 5.1 ± 0.6 | 0.58 ± 0.08 | 3.9 ± 0.1 |
5 | 240 ± 32 | 9.2 ± 0.9 | 7.1 ± 0.8 | 1.8 ± 0.6 | 7.3 ± 0.3 | 0.43 ± 0.04 | 98.9 ± 0.3 | 1.5 ± 0.1 | 0.4 ± 0.2 | 34.8 ± 4.6 | 0.5 ± 0.1 | 0.3 ± 0.1 | 5.0 ± 0.6 | 0.51 ± 0.07 | 3.9 ± 0.1 | ||
−18 | 240 ± 27 | 10.1 ± 0.7 | 7.4 ± 0.5 | 2.0 ± 0.6 | 7.9 ± 0.3 | 0.47 ± 0.03 | 98.8 ± 0.4 | 1.6 ± 0.1 | 0.4 ± 0.2 | 33.3 ± 3.8 | 0.5 ± 0.1 | 0.4 ± 0.1 | 5.1 ± 0.6 | 0.54 ± 0.08 | 3.9 ± 0.1 | ||
3 | 20 | 312 ± 62 | 8.2 ± 1.3 | 4.7 ± 1.0 | 1.3 ± 0.4 | 5.0 ± 0.2 | 0.38 ± 0.06 | 99.2 ± 0.2 | 1.8 ± 0.1 | 0.3 ± 0.1 | 21.7 ± 5.5 | 0.4 ± 0.1 | 0.1 ± 0.1 | 4.5 ± 0.4 | 0.45 ± 0.06 | 3.9 ± 0.1 | |
5 | 264 ± 47 | 7.8 ± 1.1 | 4.9 ± 1.0 | 1.2 ± 0.4 | 5.6 ± 0.2 | 0.36 ± 0.05 | 99.3 ± 0.2 | 1.6 ± 0.1 | 0.3 ± 0.1 | 30.4 ± 4.4 | 0.4 ± 0.1 | 0.2 ± 0.1 | 5.0 ± 0.4 | 0.47 ± 0.07 | 3.9 ± 0.1 | ||
−18 | 264 ± 47 | 8.1 ± 1.1 | 5.2 ± 1.1 | 1.2 ± 0.4 | 5.8 ± 0.2 | 0.38 ± 0.05 | 99.3 ± 0.2 | 1.5 ± 0.1 | 0.3 ± 0.1 | 34.8 ± 3.7 | 0.3 ± 0.1 | 0.1 ± 0.1 | 5.1 ± 0.4 | 0.51 ± 0.07 | 3.9 ± 0.1 | ||
6 | 20 | 312 ± 71 | 8.0 ± 1.7 | 5.0 ± 1.5 | 1.5 ± 0.5 | 4.9 ± 0.1 | 0.37 ± 0.08 | 99.1 ± 0.3 | 2.1 ± 0.1 | 0.3 ± 0.1 | 8.7 ± 3.9 | 0.4 ± 0.1 | 0.4 ± 0.1 | 5.1 ± 0.6 | 0.47 ± 0.08 | 3.9 ± 0.1 | |
5 | 264 ± 56 | 8.5 ± 1.2 | 5.4 ± 1.1 | 1.5 ± 0.5 | 6.2 ± 0.4 | 0.40 ± 0.06 | 99.1 ± 0.3 | 2.0 ± 0.1 | 0.3 ± 0.1 | 13.0 ± 3.1 | 0.4 ± 0.1 | 0.2 ± 0.1 | 5.1 ± 0.6 | 0.56 ± 0.10 | 3.9 ± 0.1 | ||
−18 | 264 ± 49 | 7.8 ± 0.6 | 5.0 ± 0.9 | 1.3 ± 0.4 | 5.7 ± 0.6 | 0.37 ± 0.02 | 99.2 ± 0.2 | 2.0 ± 0.1 | 0.3 ± 0.1 | 13.0 ± 3.7 | 0.4 ± 0.1 | 0.2 ± 0.1 | 5.0 ± 0.6 | 0.50 ± 0.08 | 3.9 ± 0.1 |
Nature of Thermally-Dried Kefir Culture | Fermentation Temperature (°C) | Repeated Batches | Acetaldehyde (mg/L) | Ethyl Acetate (mg/L) | 1-Propanol (mg/L) | Isobutanol (mg/L) | 1-Hexanol (mg/L) | Amyl Alcohol (mg/L) | Isoamyl Alcohol (mg/L) | Methanol (mg/L) |
---|---|---|---|---|---|---|---|---|---|---|
Free cells | 30 | 1–3 | 44–88 | 20–32 | 26–37 | 33–42 | 1–3 | 18–22 | 60–87 | 9–21 |
20 | 4–6 | 20–50 | 10–36 | 23–32 | 28–38 | 2–4 | 14–22 | 51–77 | 5–18 | |
5 | 7–8 | 13–18 | 12–15 | 11–14 | 10–12 | 5–7 | 6–8 | 18–23 | 12–13 | |
Immobilized cells on apple pieces | 30 | 1–3 | 63–96 | 28–35 | 36–71 | 38–85 | 2–3 | 18–56 | 150–180 | 7–15 |
20 | 4–6 | 15–21 | 33–67 | 57–100 | 51–94 | 3 | 24–70 | 89–220 | 8–21 | |
5 | 7–8 | 18–25 | 6–7 | 23–30 | 21–25 | 2 | 11–13 | 48–55 | 5–7 | |
Immobilized cells on DCM | 30 | 1–3 | 50–97 | 18–37 | 35–61 | 38–83 | 2–3 | 17–51 | 137–166 | 4–17 |
20 | 4–6 | 7–15 | 33–65 | 56–57 | 51–67 | 1–3 | 24–40 | 89–146 | 5–8 | |
5 | 7–8 | 14–15 | 11–12 | 14–19 | 14–20 | 2 | 8–10 | 38–41 | 12–15 | |
Immobilized cells on grape skins | 30 | 1–3 | 29–41 | 7–17 | 31–44 | 30–47 | 2–3 | 14–38 | 70–133 | 10–21 |
20 | 4–6 | 9–15 | 37–56 | 32–69 | 29–58 | 1–2 | 15–42 | 60–139 | 6–14 | |
5 | 7–8 | 14–23 | 10–12 | 27–39 | 22–30 | 1–2 | 13–17 | 60–66 | 3–5 |
Compounds Detected | KI | 30 °C | 20 °C | 5 °C | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Fr | Ap | DCM | GS | Fr | Ap | DCM | GS | Fr | Ap | DCM | GS | ||
Esters | |||||||||||||
Ethyl acetate | <700 | 0.5–1.6 | 3.0–5.0 | 2.5–3.1 | 0.8–1.6 | 1.6–6.6 | 5.0–7.3 | 6.4–8.1 | 4.8–8.2 | 1.0–1.2 | 5.0–6.3 | 3.5–4.0 | 7.9–9.0 |
Ethyl propanoate | 707 | 0.1 | 0.2–0.4 | 0.2 | 0.1 | 0.0–0.1 | 0.1–0.5 | 0.1–0.4 | 0.2–0.3 | 0.2–1.0 | 0.5–0.6 | 0.2–0.5 | 0.5–0.6 |
Isobutyl acetate | 745 | Nd | Nd | Nd | Nd | Nd | 0.0–0.1 | Nd | 0.0–0.1 | Nd | 0.1 | 0.0–0.1 | Nd |
Ethyl butyrate | 803 | <0.1–0.1 | Nd | 0.0–0.1 | 0.0–0.1 | 0.1–0.3 | Nd | 0.2 | 0.0–0.1 | 0.1–0.2 | Nd | 0.2–0.3 | Nd |
3-methylbutyl acetate | 867 | 0.1–1.4 | 2.5–4.0 | 1.9–3.7 | 0.5–1.0 | 0.5–0.9 | 2.7–4.0 | 1.9–3.2 | 1.8–2.3 | 0.3–0.4 | 1.7–2.0 | 2.0–2.3 | 1.0 |
2-methylbutyl acetate | 869 | <0.1–0.1 | Nd | Nd | Nd | 0.0–0.1 | 0.0–0.2 | Nd | 0.0–0.1 | <0.1–0.1 | Nd | 0.1–1.2 | 0.0 |
Ethyl hexanoate | 1002 | 1.6–4.6 | 0.9–4.7 | 1.0–1.6 | 0.7–1.1 | 1.8–2.5 | 1.0–1.7 | 1.2–1.9 | 0.7–1.1 | 2.4–3.0 | 0.9–1.0 | 5.0–6.0 | 0.8–1.0 |
Diethyl butanedioate | 1191 | <0.1–0.3 | 1.0–2.1 | 1.0–3.5 | 0.3–1.8 | Nd | 0.1–1.3 | 1.0–1.6 | Nd | Nd | Nd | 1.2–1.5 | 0.0 |
Ethyl octanoate | 1202 | 10.6–28.6 | 4.0–8.3 | 3.1–9.0 | 2.3–6.2 | 7.1–10.4 | 4.0–6.5 | 6.2–8.9 | 1.7–6.5 | 10.0–12.0 | 2.0–2.5 | 18.7–20.1 | 3.0 |
2-phenylethyl acetate | 1263 | 1.1–2.8 | 0.9–1.4 | 39.7–55.0 | 1.1–2.3 | 0.6–1.7 | 0.4–0.5 | 3.7–5.4 | 0.6–2.9 | 0.3 | 0.1–0.2 | 0.9–1.0 | 0.7–1.0 |
Phenylethyl isobutyrate | 1360 | Nd | Nd | 0.1–0.6 | Nd | Nd | Nd | Nd | Nd | Nd | Nd | 0.1–0.2 | Nd |
Ethyl 9-decanoate | 1390 | 0.5–1.2 | 0.1–0.9 | 0.0–0.3 | Nd | 0.3–0.4 | Nd | 0.0–0.5 | Nd | 0.1 | Nd | Nd | Nd |
Ethyl decanoate | 1398 | 11.0–20.7 | 2.6–5.9 | 1.2–3.0 | 0.6–1.0 | 1.8–5.8 | 1.5–2.4 | 2.0–7.2 | 0.4–1.2 | 0.1–0.2 | 0.5–0.6 | 0.4–0.5 | 1.0–1.1 |
3-methylbutyl octanoate | 1453 | 0.0–0.1 | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd |
Ethyl dodecanoate | 1595 | 0.0–0.1 | 0.1–0.4 | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd |
Total esters | 28.0–61.5 | 19.7–29.7 | 52.3–79.3 | 8.5–13.4 | 18.3–23.9 | 15.6–24.1 | 24.5–37.0 | 10.9–20.2 | 16.0–17.1 | 12.2–12.9 | 34.6–35.4 | 15.8–16.5 | |
Organic acids | |||||||||||||
Octanoic acid | 1198 | 0.1–0.6 | Nd | 0.2–0.3 | 0.1–0.3 | 0.5–0.9 | Nd | 0.5–0.9 | Nd | 0.6–0.7 | Nd | 0.5–0.6 | Nd |
Decanoic acid | 1381 | 0.0–0.3 | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd | Nd |
Total organic acids | 0.1–0.9 | Nd | 0.2–0.3 | 0.1–0.3 | 0.5–0.9 | Nd | 0.5–0.9 | Nd | 0.6–0.7 | Nd | 0.5–0.6 | Nd | |
Alcohols | |||||||||||||
2-methyl-1-propanol (isobutanol) | <700 | 0.5–1.2 | 0.8–1.5 | 0.9–1.6 | 0.4–0.9 | 0.3–1.3 | 0.6–1.7 | 0.7–1.2 | 0.4–0.6 | Nd | 0.8–1.0 | 0.3–0.5 | 0.4–0.5 |
3-methyl-1-butanol (isoamyl alcohol) | 721 | 11.7–26.6 | 32.6–36.0 | 25.4–40.5 | 13.9–31.1 | 15.8–24.5 | 32.8–45.4 | 20.9–28.8 | 19.7–29.9 | 7.6–8.0 | 24.4–25.0 | 10.0–11.4 | 9.9–11.7 |
2-methyl-1-butanol (amyl alcohol) | 722 | 4.5–10.3 | 12.8–15.3 | 10.0–12.9 | 4.9–8.5 | 2.6–9.7 | 6.5–13.6 | 6.2–10.5 | 5.6–8.7 | 0.9–1.0 | 5.0–6.0 | 3.0–3.2 | 3.0–3.1 |
2,3-butanediol | 756 | <0.1–0.5 | Nd | 0.1–0.3 | <0.1–0.2 | 0.0–0.1 | 0.0–0.2 | Nd | Nd | 0.1 | 0.1–0.2 | Nd | Nd |
3,7-dimethyl-1,6-octadien-3-ol (linalool) | 1123 | 0.1–0.3 | Nd | Nd | 0.0–0.2 | Nd | 0.0–0.2 | Nd | 0.1–0.2 | 0.4 | Nd | 0.5 | Nd |
2-phenylethanol | 1133 | 6.5–13.6 | 16.0–20.8 | 10.0–25.8 | 8.4–9.9 | 1.4–2.3 | 3.3–7.4 | 3.4–5.2 | 3.2–8.3 | 0.9–1.0 | 1.0–1.3 | 3.7–4.0 | 1.7–2.0 |
α-terpineol | 1192 | 0.4–1.1 | 0.4–0.7 | 0.3 | 0.2–0.3 | 0.4–0.8 | 0.1–0.3 | 0.4–0.8 | 0.2–0.5 | 0.5 | 0.2–0.4 | Nd | 0.3–0.5 |
3,7-dimethyl-6-octen-1-ol (citronellol) | 1235 | 0.0–0.1 | Nd | Nd | 0.0–0.1 | 0.1 | Nd | Nd | Nd | Nd | Nd | 0.1 | Nd |
Total alcohols | 24.9–52.9 | 63.6–72.9 | 54.6–70.7 | 28.8–46.4 | 24.0–37.8 | 46.8–68.5 | 31.8–45.0 | 32.7–39.7 | 10.4–11.0 | 32.8–33.3 | 18.3–19.3 | 16.0–17.2 | |
Miscellaneous compounds | |||||||||||||
2-fluoro-1-propene | <700 | 0.3–1.0 | 0.7–1.2 | 0.4–1.0 | 0.2–0.6 | 1.1–1.6 | 0.3–1.1 | 0.7–1.4 | 0.3–0.9 | Nd | 0.5–0.6 | 0.1 | 0.2 |
2,5-dimethyl-furan | 704 | Nd | Nd | Nd | Nd | 0.0–<0.1 | Nd | Nd | 0.0–<0.1 | 0.1 | 0.1 | 0.1 | Nd |
1,1-diethoxy-ethane (acetal) | 716 | 0.3–1.4 | 0.9–4.4 | 1.3–3.5 | 0.1–0.6 | 0.9–2.1 | Nd | Nd | 0.0–0.1 | Nd | 1.2–1.5 | 0.1 | 0.2 |
1,3,5-trimethyl-benzene (mesitylene) | 956 | 0.0–0.1 | Nd | Nd | 0.0–0.1 | 0.0–0.1 | 0.0–0.1 | Nd | 0.0–0.1 | 0.1 | Nd | 0.1–0.2 | Nd |
1,3-bis(1,1-dimethylethyl)-benzene (m-Di-tert-butylbenzene) | 1258 | 0.1–0.7 | 0.2–1.4 | 0.2–0.8 | 0.1–0.4 | 0.3–0.6 | 0.3–0.4 | 0.4–0.6 | 0.3–0.8 | 0.2 | Nd | Nd | 0.2–0.3 |
Total miscellaneous compounds | 1.5–2.5 | 3.1–5.3 | 1.9–4.9 | 0.8–1.4 | 2.3–4.2 | 0.8–1.5 | 1.1–1.8 | 0.9–1.6 | 0.4 | 1.9–2.1 | 0.5 | 0.6–0.7 | |
Total volatiles | 58.5–93.4 | 88.9–105.7 | 121.7–155.1 | 39.1–60.7 | 46.1–66.4 | 63.1–93.9 | 58.1–75.2 | 44.5–60.9 | 28.0–28.6 | 47.6 | 54.7–54.9 | 33.1–33.7 |
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Nikolaou, A.; Sgouros, G.; Santarmaki, V.; Mitropoulou, G.; Kourkoutas, Y. Preliminary Evaluation of the Use of Thermally-Dried Immobilized Kefir Cells in Low Alcohol Winemaking. Appl. Sci. 2022, 12, 6176. https://doi.org/10.3390/app12126176
Nikolaou A, Sgouros G, Santarmaki V, Mitropoulou G, Kourkoutas Y. Preliminary Evaluation of the Use of Thermally-Dried Immobilized Kefir Cells in Low Alcohol Winemaking. Applied Sciences. 2022; 12(12):6176. https://doi.org/10.3390/app12126176
Chicago/Turabian StyleNikolaou, Anastasios, Georgios Sgouros, Valentini Santarmaki, Gregoria Mitropoulou, and Yiannis Kourkoutas. 2022. "Preliminary Evaluation of the Use of Thermally-Dried Immobilized Kefir Cells in Low Alcohol Winemaking" Applied Sciences 12, no. 12: 6176. https://doi.org/10.3390/app12126176