Comparative Exoproteome Analysis of Streptococcus suis Human Isolates
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains, Culture Conditions and the Preparation of Exoproteomes
2.2. Sample Preparation for Proteomic Analysis
2.3. LC-MS/MS Analysis and Protein Identification through a Database Search
2.4. Computational Analysis of the Protein Sequences
2.5. Data and Statistical Analysis
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein Category a | #b Identified Proteins | #b Predicted Proteins in S. suis BM407 Genome | Identified/Predicted (%) |
---|---|---|---|
Lipoprotein | 33 | 40 | 82.5 |
Cell wall | 19 | 20 | 95 |
Secreted | 9 | 18 | 50 |
Membrane | 83 | 479 | 17.3 |
Cytoplasmic | 680 | 1375 | 49.4 |
Total | 824 | 1932 | 42.7 |
Protein Locus | Protein Annotation | VaxiJen Score a | # Strains b |
---|---|---|---|
SSUBM407_0206 | Putative exported protein | 0.6462 | 5 |
SSUBM407_0452 | Putative exported protein | 0.6648 | 6 |
SSUBM407_0640 | Putative exported protein | 0.4292 | 3 |
SSUBM407_0662 | Putative N-acetylmuramoyl-L-alanine amidase | 0.8672 | 6 |
SSUBM407_1229 | Putative exported protein | 0.5271 | 1 |
SSUBM407_1493 | Putative exported protein | 0.6369 | 6 |
SSUBM407_1726 | LytR family regulatory protein | 0.5444 | 3 |
SSUBM407_1898 | UTP--glucose-1-phosphate uridylyltransferase | 0.4444 | 6 |
SSUBM407_2032 | Serine protease | 0.5736 | 6 |
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de la Torre, E.P.; Rodríguez-Franco, A.; Rodríguez-Ortega, M.J. Comparative Exoproteome Analysis of Streptococcus suis Human Isolates. Microorganisms 2021, 9, 1287. https://doi.org/10.3390/microorganisms9061287
de la Torre EP, Rodríguez-Franco A, Rodríguez-Ortega MJ. Comparative Exoproteome Analysis of Streptococcus suis Human Isolates. Microorganisms. 2021; 9(6):1287. https://doi.org/10.3390/microorganisms9061287
Chicago/Turabian Stylede la Torre, Esther Prados, Antonio Rodríguez-Franco, and Manuel J. Rodríguez-Ortega. 2021. "Comparative Exoproteome Analysis of Streptococcus suis Human Isolates" Microorganisms 9, no. 6: 1287. https://doi.org/10.3390/microorganisms9061287