Antifungal Activities of Bacillus mojavensis BQ-33 towards the Kiwifruit Black Spot Disease Caused by the Fungal Pathogen Didymella glomerata
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Pathogen
2.2. Rhizobacteria Isolation and Antifungal Activity Evaluation
2.3. Identification of the BQ-33 Strain
2.4. Preparation of QB-33 the Sterile Supernatant (SS)
2.5. Test of Antifungal Activity of BQ-33 and Its SS
2.6. Chitinase and β-1,3-Glucanase Activity Assays
2.7. Changes in Cell Permeability
2.8. Effects of SS on Cell Structures of Fungal Pathogens
2.8.1. Effects on Cell Walls
2.8.2. Effects on Cell Membranes
2.9. In Vivo Control Effect of SS
2.10. Statistical Analyses
3. Results
3.1. Screening of Biocontrol Bacteria
3.2. Identification of Strain BQ-33
3.3. Antifungal Spectrum of BQ-33 Regarding Kiwifruit Diseases
3.4. Enzymatic Activities of BQ-33 SS
3.5. Effect of SS on the Permeability of D. glomerata Cells
3.6. BQ-33 SS Influence on Fungal Cells
3.7. Biocontrol Efficacy of BQ-33 SS towards Kiwifruit Black Spot Disease
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Pathogen | Host Plant | Disease Type |
---|---|---|---|
1 | Didymella glomerata | Kiwifriut | Black spot [6] |
2 | Botryosphaeria dothidea | Apple | Ring rot [31] |
3 | Alternaria alternata | Prunus salicina | Leaf spot [32] |
4 | Fusarium oxysporum | Gastrodia elata | Tuber rot [33] |
5 | Phomopsis cauloides | Kiwifriut | Soft rot [34] |
Pathogen | Inhibition Ratio by BQ-33 Suspension (%) | Inhibition Ratio by SS (%) |
---|---|---|
Didymella glomerata | 81.26 ± 0.47 a | 83.43 ± 0.35 a |
Botryosphaeria dothidea | 73.62 ± 1.43 c | 74.19 ± 0.59 c |
Alternaria alternata | 77.45 ± 1.18 b | 78.86 ± 0.55 b |
Fusarium oxysporum | 70.11 ± 0.92 d | 72.63 ± 0.56 c |
Phomopsis cauloides | 75.73 ± 0.78 bc | 77.13 ± 0.67 b |
Treatment | Lesion Diameter (mm) | Control Efficacy (%) |
---|---|---|
Control | 20.62 ± 0.66 a | - |
100 mL L−1 | 10.62 ± 0.29 b | 47.52 ± 0.02 c |
200 mL L−1 | 5.12 ± 0.13 c | 74.75 ± 0.01 b |
400 mL L−1 | 0 ± 0 d | 100 ± 0 a |
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Wang, B.; Lei, X.; Chen, J.; Li, W.; Long, Y.; Wang, W. Antifungal Activities of Bacillus mojavensis BQ-33 towards the Kiwifruit Black Spot Disease Caused by the Fungal Pathogen Didymella glomerata. Microorganisms 2022, 10, 2085. https://doi.org/10.3390/microorganisms10102085
Wang B, Lei X, Chen J, Li W, Long Y, Wang W. Antifungal Activities of Bacillus mojavensis BQ-33 towards the Kiwifruit Black Spot Disease Caused by the Fungal Pathogen Didymella glomerata. Microorganisms. 2022; 10(10):2085. https://doi.org/10.3390/microorganisms10102085
Chicago/Turabian StyleWang, Bingce, Xia Lei, Jia Chen, Wenzhi Li, Youhua Long, and Weizhen Wang. 2022. "Antifungal Activities of Bacillus mojavensis BQ-33 towards the Kiwifruit Black Spot Disease Caused by the Fungal Pathogen Didymella glomerata" Microorganisms 10, no. 10: 2085. https://doi.org/10.3390/microorganisms10102085