Influence of Biochar on Soil Nutrients and Associated Rhizobacterial Communities of Mountainous Apple Trees in Northern Loess Plateau China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Soil and Biochar
2.2. Experimental Design
2.3. Sample Collection and Laboratory Analysis
2.4. Soil pH, Organic C, and N Analysis
2.5. Measurement of Soil Enzymes’ Activities
2.6. DNA Extraction and Sequencing
2.7. Processing of Illumina Sequencing Data
2.8. Alpha and Beta Diversity Analysis
2.9. Statistical Analysis
3. Results
3.1. Soil Physiochemical Properties and Enzymatic Activities
3.2. Composition and Community Structure of Rhizobacterial Microbiome
3.3. Diversity and Species Richness of Rhizobacterial Microbiome
3.4. The Similarity of the Rhizobacterial Microbiomes
3.5. Spearmen Correlation Analysis among Major Bacterial Phyla and Environmental Factors
4. Discussion
4.1. Soil Physio-Biochemical Properties
4.2. Impacts of Biochar on Composition and Community Structure
4.3. Impact of Biochar on the Soil Bacterial Alpha and Beta Diversity
4.4. Correlation of Bacterial Communities and Environmental Factors
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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PCR Reaction System (50 µL) | Addition (µL) |
---|---|
2X Premix Taq | 25 |
Forward primer (5 µM) | 1 |
Reverse primer (5 µM) | 1 |
DNA Template | 3 |
Deionized distilled H2O | 20 |
Stages | No. of Cycle | Temperature (°C) | Time (min) |
---|---|---|---|
Initial denaturation | 1 | 94 | 5 |
Second denaturation | 30 | 94 | 0.3 |
Annealing | 1 | 52 | 0.3 |
Initial extension | 1 | 72 | 0.3 |
Final extension | 1 | 72 | 10 |
3 Months | |||||||||
---|---|---|---|---|---|---|---|---|---|
Treat | Soil pH | SOM | STN | NO3−-N | NH4+-N | UR | CAT | ALP | SC |
Ck | 8.91 ± 0.06 a | 8.48 ± 0.19 a | 0.42 ± 0.05 a | 3.93 ± 0.15 a | 67.50 ± 3.90 a | 0.30 ± 0.03 a | 3.95 ± 0.09 a | 0.99 ± 0.05 a | 6.11 ± 0.35 a |
T1 | 8.93 ± 0.14 a | 8.75 ± 0.45 a | 0.53 ± 0.06 abc | 4.43 ± 0.82 a | 71.68 ± 2.39 a | 0.33 ± 0.05 a | 4.01 ± 0.07 ab | 1.09 ± 0.03 ab | 6.13 ± 0.40 a |
T2 | 8.98 ± 0.05 ab | 9.41 ± 0.30 b | 0.47 ± 0.07 ab | 6.43 ± 1.02 b | 78.69 ± 3.33 a | 0.51 ± 0.03 c | 4.09 ± 0.05 b | 1.12 ± 0.04 bc | 6.45 ± 0.16 ab |
T3 | 9.03 ± 0.02 ab | 10.30 ± 0.15 c | 0.55 ± 0.06 abc | 7.63 ± 1.04 b | 81.47 ± 5.20 b | 0.42 ± 0.06 b | 4.14 ± 0.06 bc | 1.19 ± 0.02 bc | 6.70 ± 1.18 ab |
T4 | 9.09 ± 0.02 b | 10.89 ± 0.22 d | 0.61 ± 0.13 bc | 9.43 ± 0.72 c | 83.82 ± 2.50 b | 0.45 ± 0.05 bc | 4.25 ± 0.04 c | 1.22 ± 0.09 cd | 7.11 ± 0.44 ab |
T5 | 9.11 ± 0.06 b | 11.52 ± 0.22 e | 0.67 ± 0.10 c | 10.42 ± 1.13 c | 84.77 ± 2.64 b | 0.53 ± 0.03 c | 4.30 ± 0.05 cd | 1.31 ± 0.06 d | 7.52 ± 0.27 b |
6 Months | |||||||||
Ck | 8.95 ± 0.06 a | 8.56 ± 0.26 a | 0.46 ± 0.04 a | 3.98 ± 0.14 a | 68.20 ± 5.03 a | 0.32 ± 0.03 a | 3.94 ± 0.05 a | 1.02 ± 0.02 a | 6.15 ± 0.49 a |
T1 | 8.98 ± 0.06 ab | 8.78 ± 0.39 a | 0.52 ± 0.07 ab | 4.49 ± 0.20 a | 73.44 ± 8.14 a | 0.35 ± 0.07 ab | 4.05 ± 0.07 b | 1.12 ± 0.03 ab | 6.20 ± 0.52 a |
T2 | 9.02 ± 0.07 abc | 9.95 ± 0.35 b | 0.55 ± 0.07 abc | 6.60 ± 0.84 b | 81.95 ± 2.49 b | 0.54 ± 0.04 d | 4.09 ± 0.03 b | 1.17 ± 0.04 bc | 6.58 ± 0.22 ab |
T3 | 9.08 ± 0.04 bc | 10.62 ± 0.55 bc | 0.59 ± 0.05 bc | 7.77 ± 1.11 b | 84.04 ± 4.51 b | 0.43 ± 0.06 bc | 4.18 ± 0.06 c | 1.22 ± 0.03 bcd | 6.79 ± 1.13 ab |
T4 | 9.11 ± 0.04 c | 11.28 ± 0.17 cd | 0.67 ± 0.08 cd | 9.55 ± 0.58 c | 88.52 ± 1.50 b | 0.49 ± 0.06 cd | 4.27 ± 0.05 cd | 1.27 ± 0.10 cd | 7.28 ± 0.93 ab |
T5 | 9.14 ± 0.08 c | 11.69 ± 0.50 d | 0.74 ± 0.06 d | 10.58 ± 1.55 c | 88.57 ± 3.29 b | 0.57 ± 0.05 d | 4.32 ± 0.06 d | 1.33 ± 0.07 d | 7.85 ± 1.22 b |
9 Months | |||||||||
Ck | 8.99 ± 0.01 a | 8.75 ± 0.33 a | 0.49 ± 0.05 a | 4.10 ± 0.33 a | 69.13 ± 14.07 a | 0.34 ± 0.04 a | 3.93 ± 0.09 a | 1.03 ± 0.06 a | 6.18 ± 1.11 a |
T1 | 9.04 ± 0.03 a | 8.86 ± 1.45 a | 0.57 ± 0.08 a | 4.53 ± 1.16 a | 74.79 ± 7.56 ab | 0.36 ± 0.03 a | 4.04 ± 0.07 ab | 1.13 ± 0.09 ab | 6.24 ± 0.39 a |
T2 | 9.06 ± 0.03 ab | 10.19 ± 0.28 b | 0.56 ± 0.07 a | 6.73 ± 0.92 b | 84.06 ± 4.54 bc | 0.60 ± 0.07 c | 4.13 ± 0.05 bc | 1.21 ± 0.03 bc | 6.67 ± 0.41 b |
T3 | 9.13 ± 0.01 bc | 11.56 ± 0.19 c | 0.62 ± 0.03 ab | 8.09 ± 1.68 bc | 85.12 ± 3.94 bc | 0.47 ± 0.05 b | 4.20 ± 0.06 cd | 1.27 ± 0.04 bc | 6.82 ± 1.06 b |
T4 | 9.16 ± 0.09 bc | 11.68 ± 0.40 c | 0.71 ± 0.09 bc | 9.78 ± 1.22 cd | 89.66 ± 2.81 c | 0.52 ± 0.06 bc | 4.31 ± 0.07 de | 1.32 ± 0.14 c | 7.43 ± 2.03 bc |
T5 | 9.19 ± 0.08 c | 12.03 ± 0.57 c | 0.81 ± 0.07 c | 10.82 ± 0.69 d | 90.30 ± 4.82 c | 0.61 ± 0.04 c | 4.39 ± 0.09 e | 1.35 ± 0.08 c | 7.97 ± 0.87 c |
12 Months | |||||||||
Ck | 9.01 ± 0.01 a | 8.82 ± 0.21 a | 0.53 ± 0.06 a | 4.14 ± 1.82 a | 69.81 ± 30.94 a | 0.35 ± 0.05 a | 3.95 ± 0.11 a | 1.06 ± 0.03 a | 6.20 ± 1.37 a |
T1 | 9.08 ± 0.04 ab | 8.92 ± 1.52 a | 0.59 ± 0.16 ab | 4.64 ± 2.66 a | 76.16 ± 9.01 a | 0.38 ± 0.05 a | 4.08 ± 0.12 ab | 1.19 ± 0.28 a | 6.33 ± 1.35 a |
T2 | 9.10 ± 0.05 abc | 10.47 ± 0.56 b | 0.59 ± 0.09 ab | 7.32 ± 3.10 ab | 86.56 ± 5.01 b | 0.63 ± 0.06 d | 4.15 ± 0.11 abc | 1.33 ± 0.09 b | 6.83 ± 0.25 b |
T3 | 9.16 ± 0.01 bc | 11.65 ± 0.27 bc | 0.65 ± 0.07 ab | 8.52 ± 5.20 ab | 87.15 ± 8.11 b | 0.51 ± 0.04 b | 4.24 ± 0.11 bcd | 1.31 ± 0.09 b | 6.90 ± 1.16 b |
T4 | 9.19 ± 0.12 bc | 12.09 ± 0.27 c | 0.73 ± 0.12 bc | 10.13 ± 3.41 ab | 91.11 ± 4.58 b | 0.58 ± 0.10 bc | 4.33 ± 0.09 cd | 1.35 ± 0.13 b | 7.58 ± 0.94 c |
T5 | 9.22 ± 0.09 c | 12.38 ± 0.15 c | 0.87 ± 0.08 c | 11.17 ± 2.85 b | 92.97 ± 5.94 b | 0.65 ± 0.04 d | 4.40 ± 0.08 d | 1.41 ± 0.31 bc | 8.04 ± 1.41 cd |
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Ahmad, R.; Gao, J.; Gao, Z.; Khan, A.; Ali, I.; Fahad, S. Influence of Biochar on Soil Nutrients and Associated Rhizobacterial Communities of Mountainous Apple Trees in Northern Loess Plateau China. Microorganisms 2022, 10, 2078. https://doi.org/10.3390/microorganisms10102078
Ahmad R, Gao J, Gao Z, Khan A, Ali I, Fahad S. Influence of Biochar on Soil Nutrients and Associated Rhizobacterial Communities of Mountainous Apple Trees in Northern Loess Plateau China. Microorganisms. 2022; 10(10):2078. https://doi.org/10.3390/microorganisms10102078
Chicago/Turabian StyleAhmad, Rafiq, Jianen Gao, Zhe Gao, Abdullah Khan, Izhar Ali, and Shah Fahad. 2022. "Influence of Biochar on Soil Nutrients and Associated Rhizobacterial Communities of Mountainous Apple Trees in Northern Loess Plateau China" Microorganisms 10, no. 10: 2078. https://doi.org/10.3390/microorganisms10102078