Neospora caninum: Structure and Fate of Multinucleated Complexes Induced by the Bumped Kinase Inhibitor BKI-1294
, Wesley C. Van Voorhis 4, Joachim Müller 1 and Andrew Hemphill 1,*Round 1
Reviewer 1 Report
Innovative and interesting work produced by renowned authors in the field.
In my opinion, the manuscript is acceptable for publication, pending some minor adaptations.
L39 – display keywords alphabetically
L44 – Apicomplexa (uppercase)
L44 – rewrite to read as: The phylum Apicomplexa includes important pathogens of animals and/or man, including Plasmodium falciparum, Cryptosporidium parvum, Eimeria spp., Babesia spp., Theileria spp., Toxoplasma gondii and Neospora caninum.
L48 – write: T. gondii… N. caninum
L50 – delete comma after sporulation
L52 – insert comma after and,
L59 – abbreviate generic names for those above-mentioned species – is there any criteria to display these species by this order?
L76 – write: N. caninum
Explain the meaning of abbreviations at their first use, e.g. TEM and HFF
Figure 6 – overlay is missing for C
L349 – adapt: Plasmodium spp.
L358 – S. neurona
Ref. 9 – display Toxoplasma in italic type
Ref. 11 - In vitro and in vivo effects of the bumped kinase
Author Response
Thank you for your positive review. Please find below our point-by-point response:
L39 – display keywords alphabetically
Response: keywords arranged alphabetically
L44 – Apicomplexa (uppercase)
Response: done
L44 – rewrite to read as: The phylum Apicomplexa includes important pathogens of animals and/or man, including Plasmodium falciparum, Cryptosporidium parvum, Eimeria spp., Babesia spp., Theileria spp., Toxoplasma gondii and Neospora caninum.
Response: changed as requested
L48 – write: T. gondii… N. caninum
Response: done
L50 – delete comma after sporulation
Response: done
L52 – insert comma after and,
Response: done
L59 – abbreviate generic names for those above-mentioned species – is there any criteria to display these species by this order?
Response: done. There are no specific criteria for the order.
L76 – write: N. caninum
Response: done
Explain the meaning of abbreviations at their first use, e.g. TEM and HFF
Response: done so in the first paragraph of the results section
Figure 6 – overlay is missing for C
Response: the overlay is not available. However, since there is no staining in panel A, we believe it is not explicitly necessary to show this.
L349 – adapt: Plasmodium spp.
Response: done
L358 – S. neurona
Response: done
Ref. 9 – display Toxoplasma in italic type
Response: done
Ref. 11 - In vitro and in vivo effects of the bumped kinase
Response: corrected
Reviewer 2 Report
I have examined “Neospora caninum: Structure and fate of multinucleated complexes
induced by the bumped kinase inhibitor BKI-1294”. I find it an excellent paper that deals with the phenotypic effects of drug treatment using a BKI on tachyzoite development. The finding of multinucleate stages in host cells but no multinucleate host cells is interesting. This has been seen in Toxoplasma gondii treated with diclazuril but never examined in detail like the present study. The descriptions and TEM micrographs are very good.
Author Response
Thank you for your positive review!
