Next Article in Journal
Group B Streptococcus among Pregnant Women and Neonates in Saudi Arabia: A Systemic Review
Previous Article in Journal
Do or Die: HPV E5, E6 and E7 in Cell Death Evasion
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pathogens
Volume 11
Issue 9
10.3390/pathogens11091028
pathogens-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Susceptibility of Different Life Stages of Kudzu Bug Megacopta cribraria (F.) (Hemiptera: Plataspidae) to Two Different Native Strains of Beauveria bassiana

by
James Paul Glover
1,
Maribel Portilla
1,*,
Katherine Parys
2,
Clint Allen
1,
Justin George
1 and
Gadi V. P. Reddy
1
1
Southern Insect Management Research Unit, United State Department of Agriculture-Agricultural Research Service, Stoneville, MS 38776, USA
2
Pollinator Health in South Crop Ecosystems Research Unit, United State Department of Agriculture-Agricultural Service, Stoneville, MS 38776, USA
*
Author to whom correspondence should be addressed.
Pathogens 2022, 11(9), 1028; https://doi.org/10.3390/pathogens11091028
Submission received: 29 July 2022 / Revised: 5 September 2022 / Accepted: 6 September 2022 / Published: 9 September 2022
(This article belongs to the Section Fungal Pathogens)
Browse Figures
Review Reports Versions Notes

Abstract

:
This is the first study that examined and compared the survival, LC50, and RR50 estimates of Megacopta cribraria F. (Hemiptera: Plataspidae) nymphs and adults that were exposed to two native Beauveria bassiana isolates (Previously codified as NI8 and KUDSC strains) at four concentrations. The greatest reduction in survival and mortality was observed primarily on or after 10 d post-exposure to B. bassiana isolates. Survival of early instars (2nd, 3rd) were not affected by either strains or concentration at 3 d and 5 d post-exposure. Survival of later instars (5th) and adults was significantly reduced when exposed to the KUDSC strain at all concentrations. Comparison of dose–mortality values (LC50) using resistance ratios (RR50) were significantly different between life stages of the kudzu bug for both strains of B. bassiana. The LC50 values showed that kudzu bug adults are more susceptible than any other life stage when exposed to either strain. The KUDSC strain was more pathogenic than NI8 10 d after exposure, but NI8 exhibited significantly higher pathogenicity than KUDSC 20 d after exposure. Our results suggest potential field application of B. bassiana for kudzu bug control and their integration into pest management strategies to suppress them before they cause economic damage to soybean crops.

1. Introduction

Kudzu bug, Megacopta cribraria (F.) (Hemiptera: Plataspidae), is an introduced urban and agricultural pest of soybeans (Glycine max (L.) Merrill) (Fabales: Fabaceae) grown in the southeastern United States. Native to southeastern Asia, kudzu bugs were first identified in the United States as an urban pest in northeastern Georgia in 2009, and one of the two representatives of the hemipteran family Plataspidae introduced to the Americas [1,2]. The geographic range of kudzu bugs expanded rapidly within the United States, spreading as far west as Louisiana and north to Maryland by 2015, occupying 652 counties in the United States by 2017, and west into Texas by 2021 [3,4,5]. Models indicate that much of Central America, South America, and the eastern United States are susceptible to future invasion by this species [6], but thermal limits should restrict establishment north of the state of Maryland [7]. Assumed to have established from a single introductory location in Georgia, kudzu bugs also vary in size and flight potential in relation to the distance from the original introduction [8,9].
Feeding by kudzu bugs can cause yield losses in untreated soybean across the southeastern United States [10]. Kudzu bugs, both adults and nymphs, feed on kudzu variety lobata (Pueraria montana (Lour.) Merr. (Fabales: Fabaceae) (Willd), as the name implies, along with soybeans and a variety of other legumes [1,11,12]. Adults overwinter in the leaf litter in woody or forested areas, moving into the litter in late November and early December, and are often observed aggregating on the outside walls of houses and other urban structures [7,13]. The species is bivoltine in the United States, and both generations have been observed using kudzu and soybean as host plants [14,15,16]. Kudzu bugs feeding on soybean are capable of causing severe reductions in yield, up to 60% [15]. Options for chemical control are available for both in-field and structural use [17,18].
Organic bean growers in the southeastern United States may have severely damaged or destroyed crops by and have limited control options for kudzu bugs [19]. Only a few natural enemies of kudzu bugs have been found in the United States [20,21,22,23]. The pathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. (Cordycipitaceae) has been observed to cause epizootic outbreaks in kudzu bug populations across the region [10,19,24,25,26,27].
In 2005, a native Mississippi Delta strain of B. bassiana (NI8) was isolated from Lygus lineolaris Palisot de Beauvois (Hemiptera: Miridae) and was effective in measurable pest suppression of L. lineolaris [28]. A native South Carolina strain of B. bassiana originally isolated from kudzu bugs was identified and codified as KUDSC in 2015 and successfully established and colonized insects [25]. This study is the first investigation that examined and compared the survival, mortality, dose–mortality values (LC50), and resistance ratio (RR50) estimates of nymphs and adults of M. cribraria exposed to four concentrations of both NI8 and KUDSC native strains of B. bassiana.

2. Materials and Methods

2.1. Kudzu Bug Collections

Kudzu bugs of all life stages were collected during June of 2015 from kudzu plants in Yazoo County, located south of the town of Yazoo City, Mississippi. Insects were collected using a 15-inch diameter sweep net from large patches of kudzu on the roadside, and individuals were returned to the laboratory at the USDA-ARS in Stoneville, Mississippi. After collection, insects were sorted by instar and maintained overnight at 27 °C in screened insect observation cages (Popup Rearing Cages 1466 PB) (30 × 30 × 30 cm, BugdormTM, BioQuip Products, Rancho Dominguez, CA, USA) containing kudzu plants for food until experiments were conducted.

2.2. B. bassiana Spore Preparation

A strain of B. bassiana (NI8), originally isolated from L. lineolaris and native to the Mississippi Delta [28], is regularly cultured at the USDA-ARS Southern Insect Management Research Unit in Stoneville, Mississippi. Strain KUDSC was originally isolated from kudzu bugs collected at the Clemson University Edisto Research and Education Center in Blackville, South Carolina, SC, USA. [25]. Technical grade powder of both strains was produced using a medium-scale biphasic culture system for solid-substrate fermentation [25,29].

2.3. Bioassay Procedures

Bioassays were conducted using both native strains of B. bassiana on four nymphal instars (2nd–5th) and kudzu bug adults. At first, instar nymphs were not included in this study due to high handling losses during field collections. Detailed methodology for spore germination and calculation for spores/mL and spores/mm2 have been published previously [25,29,30,31]. Each treatment suspension for both isolates (7 × 104, 7 × 105, 7 × 106, 7 × 107 spores/mL) (NI8: 380, 38, 3.8, 0.38 spores/mm2) (KUDSC: 350, 35, 3.5, 0.35 spores/mm2) and water control were applied to each of three replicated groups of 30 kudzu bugs, for a total of 900 individuals of each specific age category, and an overall total of 2700 individual of kudzu bugs. For each replicated group, treatments were applied in a 6 mL spray volume from the lowest concentration to highest concentration, and nozzles were changed between each treatment to avoid cross-contamination. As described in [31], conidia suspencions were applied with specially designed spray tower equipped with an air atomizing nozzle ¼ J, fluid cap 2850, and air cap 70 (FN5925-001-001A, Wheaton, IL, USA). After each application, the cohort of kudzu bugs was released into an observation insect cage. To allow insects to dry post-spray, a single cage was utilized for each replicate and treatment combination. Once dry, insects that had been sprayed were placed individually into plastic cups containing a solid diet developed for plant-feeding hemipterans [31,32]. Following the spray exposure, kudzu bugs were held for 20 d in an environmental chamber at 27 °C, 65% R.H., with a photoperiod of 12:12 (L:D) h. Insects were considered dead when no movement was observed after prodding. Dead insects were kept individually in their original cup and checked daily for sporulation of B. bassiana fungus on the cadaver.

2.4. Statistical Analyses

Experiments were analyzed as a randomized complete block with the factorial arrangement in PROC GLIMMIX [33]. Nine treatments at each life stage examined included B. bassiana strains NI8 and KUDSC, and the water control. Each treatment combination was repeated three times. Nonparametric estimates of survival were compared between treatments using PROC LIFETEST. Mortality was corrected for control effects by using Abbot’s formula [34]. Slopes, LC50, and RR50 estimates of B. bassiana strains were obtained from the corrected data from bioassays using PROC PROBIT [33].

3. Results

3.1. Nymph and Adult Survival and Sporulation

Significant differences were observed in the number of days until death after exposure for all kudzu bug developmental stages. There were statistically significant differences in all life stages among concentrations for either strain (−2log-rank test of equality over strata) (Figure 1, Figure 2 and Figure 3). Survival was not affected by either of the B. bassiana isolates or concentrations assayed for any life stage in this experiment one day post-exposure except for adults treated with the KUDSC strain. Regardless of treatment combination, differences in survival never increased from day 15 through day 20 in this study (Figure 1, Figure 2 and Figure 3). The greatest reduction in survival was observed primarily on or after 10 d post-exposure to the B. bassiana isolates. Survival of 2nd and 3rd instars were not affected by either strain or concentration investigated at 3 d and 5 d post-exposure (Figure 1). Survival of 5th instar (Figure 2D) and adults (Figure 3B) was significantly reduced when exposed to the KUDSC strain at all concentrations. No mortality of late instars or adults were observed with the NI8 strain 5 d after exposure. Little variation was observed in the average number of days for cadavers to sporulate irrespective of isolate and concentration, ranging from 2 to 3 d for nymphal stages and 2 to 5 d for adults.

3.2. Nymphal and Adult Mortality

3.2.1. Second and Third Instars of Kudzu Bug

A significantly different strain–concentration interaction was observed on day 5 for 2nd instar nymphs (F = 2.03; df = 8, 809; p < 0.0281). No significant interaction was observed for 3rd instar nymphs on day 5 (p < 0.05). Unexpected early mortality was observed in 2nd instar bugs exposed to isolate KUDSC strain at the lowest concentration (7 × 104) 5 d after exposure (22.22 ± 1.56) (Figure 1B). That mortality was obtained two days before the higher concentrations (7 × 106 and 7 × 107) for both strains (Figure 1A,B). However, mortality values remained low for the next 5 d, with a maximum mortality of 37.7 ± 5.1 to day 20, with no significant differences compared with the KUDSC 7 × 105 concentration (37.0 ± 5.1) (Figure 1B/Table 1). Mortality of 2nd and 3rd instars with higher concentration started at day 5 for NI8 and day 6 for KUDSC and increased rapidly to day 15, and their mortality values for both nymphal stages were evidently higher with NI8 than KUDSC (Figure 1). Significant differences in mortality were observed for both instars at day 10 (2nd instar: F = 13.35; df = 8, 809; p < 0.0001) (3rd instar: F = 9.25; df = 8, 809; p < 0.0001) and 20 d (2nd Instar: F = 13.69; df = 8, 809; p < 0.0001) (3rd instar: F = 17.13; df = 8, 809; p < 0.0001) after exposure (Table 1).

3.2.2. Fourth and Fifth Instars of Kudzu Bug

Similar to the 2nd instars, 4th instar nymphs were not significantly affected by B. bassiana isolate or concentration on day 5 after exposure (F = 1.00; df = 8, 809; p < 0.4721) (Figure 2A,B). However, there were statistically significant differences in mortality when strains and concentrations were compared on day 10 (F = 3.22; df = 8, 809; p < 0.0001) and 20 (F = 6.76; df = 8, 809; p < 0.0001). The mortality trend for 4th and 5th instars exposed to both isolates had similar interaction for all concentrations. The mortality value for 4th instar nymphs slightly increased when bugs were exposed to the KUDSC strain with the highest concentration 20 d after exposure, with 85.56 ± 3.73% and 95.56 ± 2.18% for NI8 and KUDSC, respectively. Mortalities for 5th instars slightly increased when the bugs were exposed to NI8 with the highest concentration 20 d after exposure with 72.22 ± 5.28% and 66.67 ± 4.50% for NI8 and KUDSC, respectively, showing highly significant differences among strains (Figure 2C,D) (Table 1). Although 5th instars were highly susceptible to the KUDSC strain, showing mortality from day 1 (Figure 1D), mortality values were not higher than those observed for 2nd (73.33 ± 4.70%), 3rd (85.65 ± 3.71%), and 4th (95.56 ± 2.18%) instars 20 d after exposure. The 4th and 5th instars exposed to the lowest concentration (7 × 104, 7 × 105) did not significantly differ from those exposed to the water control (Table 2). Significant differences in mortality were observed for the 5th instar at day 5 (F = 28.05; df = 8, 809; p < 0.0001), 10 (F = 9.29; df = 8, 809; p < 0.0001), and 20 d (F = 11.63; df = 8, 809; p < 0.0001) post-exposure (Table 1).

3.2.3. Adults of Kudzu Bug

The mortality trend of kudzu bug adults differed from nymphal stages when exposed to NI8 and KUDSC strains. No significant differences were found among lower concentrations of NI8 (7 × 104, 7 × 105, 7 × 106), and no significant differences were observed with any concentrations of the KUDSC strain (Figure 3A,B). Similar to 5th instars, adult mortality started 1 d after the insects were exposed to any concentration of the KUDSC strain. However, at the end of the study, the highest NI8 concentration had greater mortality (90.00 ± 3.18%) than the KUDSC strain (86.67 ± 3.60%) (Table 1). Fifth instar nymphs were highly susceptible to the KUDSC strain at all test concentrations. Significant differences in mortality were observed for 5th instars at 5 d (F = 33.13; df = 8, 809; p < 0.0001), day 10 (F = 24.01; df = 8, 809; p < 0.0001), and 20 d (F = 23.82; df = 8, 809; p < 0.0001) post-exposure (Table 1).

3.3. Lethal Concentration and Resistance Ratio of Life Stages of Kudzu Bug

The LC50 values (spores/mm2), as determined by probit analysis, indicated variability among life stages of kudzu bugs treated with both strains of B. bassiana (Table 2 and Table 3). For all life stages, the probit model produced a good fit of the data among the concentrations. Comparison of dose–mortality values using resistance ratios (RR50) was significantly different between life stages of kudzu bug for both strains of B. bassiana. The LC50 values showed that kudzu bug adults are more susceptible to both strains than any other life stage. The KUDSC strain was more pathogenic than NI8 at 10 d after exposure, but NI8 was significantly higher than KUDSC 20 d after exposure. Fifth instars were found to be significantly more tolerant to NI8 than all other life stages, with a RR50 183-fold higher than adults, and 11-fold, 18-fold, and 6-fold less pathogenic when compared with 2nd, 3rd, and 4th instars, respectively (Table 3). No significant differences in pathogenicity were observed when nymphal stages were exposed to the KUDSC strain (Table 3).

4. Discussion

The data from these experiments suggest potential field usage of B. bassiana for control of kudzu bugs and their integration into pest management strategies, particularly in organic production systems where synthetic pesticides are not an option. This is unlikely to be successful in conventionally grown crops, where commercially available fungicides are known to suppress B. bassiana efficacy in the field [35] and have been reported to significantly decrease the number of sporulated kudzu bug cadavers observed in a Georgia soybean field [36]. Population dynamics of kudzu bugs in both kudzu and soybean are known to affect the levels of control achieved through application of insecticidal sprays [37], suggesting that similar follow-up studies should be done to investigate potential timing of fungal sprays both in crops and non-crop natural areas that act as population sources before kudzu bugs reach higher densities and become urban pests. The high susceptibility of all life stages of kudzu bugs observed in our study indicates that any stage can acquire lethal doses of conidia from direct spray, and a single spore/mm2 can kill adults regardless of the strain, which corroborated with data reported in another study [25] that evaluated mortality of young and old adults of kudzu bugs with two native and one commercial B. bassiana strains. Until today, no study has directly measured the pathogenicity of B. bassiana on nymphal stages of kudzu bugs; therefore, no comparable results were available for this part of the study.
While this study utilized only two strains of B. bassiana, recent work identified 42 isolates from soils in seven kudzu patches in North Carolina [38], suggesting that additional isolates could be considered for screening. Potential synergies exist with other biopesticides and biological control agents [39]. Studies indicate that host plant resistance also varies among legume species and soybean varieties [40], adding additional complexity to the system that could explain the frequent variation in fields.
While B. bassiana may not currently be a feasible component of large-scale commercial soybean production in the southeastern United States, biological control agents have additional roles alongside the assumed usages in organic crop production. Fungal biological control agents like B. bassiana could be proactively utilized when kudzu bugs and other insects form aggregations and become urban pests on structures to prevent human health hazards in the United States [19]. A similar strategy has been used in Argentina to control synthetic insecticide-resistant hemipteran pests that transmit human diseases [41]. Other potential uses may be to reduce populations of kudzu bugs in non-crop alternative hosts, similar to suggestions previously made for tarnished plant bugs (Lygus spp.) [36].

Author Contributions

Conceptualization, M.P.; methodology, M.P.; validation, M.P., K.P. and J.G.; formal analysis, M.P., K.P. and J.G; investigation, M.P.; insect collection, K.P. and C.A.; writing—original draft preparation, J.P.G.; writing—review and editing, M.P., K.P., C.A., J.G. and G.V.P.R. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Acknowledgments

Special thanks to Lou Andrews, Tabatha Nelson, Leslie Price, G. Chad Roberts, Lou Adams, Cadarius Cannon, Raksha Chatakondi, Russel Godbold, Shawnee Gundry, and Megan Holley for field and lab assistance, and to Manuela Jojoa-Portilla for data entry. Thank you to anonymous reviewers for their comments and critique before publication. This work was supported by U.S. Department of Agriculture, Agricultural Research Service, Research Project# 6066-22000-090-00D Insect Control and Resistance Management in Corn, Cotton, Sorghum, Soybean, and Sweet Potato, and Alternative Approaches to Tarnished Plant Bug Control in the Southern United States. The findings and conclusions in this publication are those of the author(s) and should not be construed to represent any official U.S.D.A. or U.S. Government determination or policy. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information. It does not imply a recommendation or endorsement by the U.S. Department of Agriculture.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Eger, J.E., Jr.; Ames, L.M.; Suiter, D.R.; Jenkins, T.M.; Rider, D.A.; Halbert, S.E. Occurrence of the Old World bug Megacopta cribraria (Fabricius) (Heteroptera: Plataspidae) in Georgia: A serious home invader and potential legume pest. Insecta Mundi 2010, 121, 1–11. [Google Scholar]
  2. Eger, J.E., Jr.; Pitcher, A.J.; Halbert, S.E.; Penca, C.; Hodges, A.C. First report of Brachyplatys subaeneus (Westwood) (Hemiptera: Heteroptera: Plataspidae) in the United States. Insecta Mundi 2020, 2020, 1–6. [Google Scholar]
  3. Dhammi, A.; van Krestchmar, J.B.; Ponnusamy, L.; Bacheler, J.S.; Reisig, D.D.; Herbert, A.; Del Pozo-Valdivia, A.I.; Roe, R.M. Biology, Pest Status, Microbiome and Control of Kudzu Bug (Hemiptera: Heteroptera: Plataspidae): A New Invasive Pest in the U.S. Int. J. Mol. Sci. 2016, 17, 1570. [Google Scholar] [CrossRef] [PubMed]
  4. GBIF. Occurrence Download. Available online: https://doi.org/10.15468/dl.fsrmtb (accessed on 10 May 2021).
  5. Liang, W.; Tran, L.; Wiggins, G.J.; Grant, J.F.; Stewart, S.D.; Washington-Allen, R. Determining Spread Rate of Kudzu Bug (Hemiptera: Plataspidae) and Its Associations with Environmental Factors in a Heterogeneous Landscape. Environ. Entomol. 2019, 48, 309–317. [Google Scholar] [CrossRef] [PubMed]
  6. Liang, W.; Tran, L.; Washington-Allen, R.; Wiggins, G.; Stewart, S.; Vogt, J.; Grant, J. Predicting the potential invasion of kudzu bug, Megacopta cribraria (Heteroptera: Plataspidae), in North and South America and determining its climatic preference. Biol. Invasions 2018, 20, 2899–2913. [Google Scholar] [CrossRef]
  7. Grant, J.I.; Lamp, W.O. Cold Tolerance of Megacopta cribraria (Hemiptera: Plataspidae): An Invasive Pest of Soybeans. Environ. Entomol. 2017, 46, 1406–1414. [Google Scholar] [CrossRef]
  8. Lovejoy, R.T.; Lozier, J.D. Core-marginal dynamics interact with sex and temperature to influence morphology of the rapidly expanding invasive kudzu bug (Megacopta cribraria). Ecol. Entomol. 2021, 46, 790–799. [Google Scholar] [CrossRef]
  9. Merwin, A.C. Flight capacity increases then declines from the core to the margins of an invasive species’ range. Biol. Lett. 2019, 15, 20190496. [Google Scholar] [CrossRef]
  10. Lahiri, S.; Reisig, D.D. Ecology and Management of Kudzu Bug (Hemiptera: Plataspidae) in Southeastern Soybeans. J. Int. Pest Manag. 2016, 7, 14. [Google Scholar] [CrossRef]
  11. Medal, J.; Halbert, S.; Santa Cruz, A.; Smith, T.; Davis, B.J. Greenhouse study to determine the host range of the kudzu bug, Megacopta cribraria (Heteroptera: Plataspidae). Fla. Entomol. 2016, 99, 303–305. [Google Scholar] [CrossRef]
  12. Yang, L.; Hu, X.P.; Golec, J.R.; Zeng, X. Effects of Legume Species and Plant Growth Stage on Attraction, Fecundity, and Development of the Kudzu Bug (Heteroptera: Plataspidae). J. Econ. Entomol. 2018, 111, 2217–2224. [Google Scholar] [CrossRef] [PubMed]
  13. Lahiri, S.; Orr, D.; Sorenson, C.E.; Cardoza, Y.J. Overwintering refuge sites for Megacopta cribraria (Hemiptera: Plataspidae). J. Entomol. Sci. 2015, 50, 69–73. [Google Scholar] [CrossRef]
  14. Del Pozo-Valdivia, A.I.; Reisig, D.D. First-generation Megacopta cribraria (Hemiptera: Plataspidae) can develop on soybeans. J. Econ. Entomol. 2013, 106, 533–535. [Google Scholar] [CrossRef] [PubMed]
  15. Seiter, N.J.; Greene, J.K.; Reay-Jones, F.P. Reduction of soybean yield components by Megacopta cribraria (Hemiptera: Plataspidae). J. Econ. Entomol. 2013, 106, 1676–1683. [Google Scholar] [CrossRef]
  16. Grant, J.I.; Lamp, W.O. Degree day requirements for Kudzu Bug (Hemiptera: Plataspidae), a pest of soybeans. J. Econ. Entomol. 2018, 111, 700–706. [Google Scholar] [CrossRef]
  17. Seiter, N.J.; Benson, E.P.; Reay-Jones, F.P.; Greene, J.K.; Zungoli, P.A. Residual efficacy of insecticides applied to exterior building material surfaces for control of nuisance infestations of Megacopta cribraria (Hemiptera: Plataspidae). J. Econ. Entomol. 2013, 106, 2448–2456. [Google Scholar] [CrossRef]
  18. Seiter, N.J.; Greene, J.K.; Reay-Jones, F.P.F.; Roberts, P.M.; All, J.N. Insecticidal control of Megacopta cribraria (Hemiptera: Plataspidae) in Soybean. J. Entomol. Sci. 2015, 50, 263–283. [Google Scholar] [CrossRef]
  19. Ruberson, J.R.; Takasu, K.; David Buntin, G.; Eger, J.E.; Gardner, W.A.; Greene, J.K.; Jenkins, T.M.; Jones, W.A.; Olson, D.M.; Roberts, P.M.; et al. From Asian curiosity to eruptive American pest: Megacopta cribraria (Hemiptera: Plataspidae) and prospects for its biological control. Appl.Entomol. Zool. 2013, 48, 3–13. [Google Scholar] [CrossRef]
  20. Golec, J.R.; Hu, X.P.; Ray, C.; Woodley, N.E. Strongygaster triangulifera (Diptera: Tachinidae) as a parasitoid of adults of the invasive Megacopta cribraria (Heteroptera: Plataspidae) in Alabama. J. Entomol. Sci. 2013, 48, 352–354. [Google Scholar] [CrossRef]
  21. Stubbins, F.L.; Agudelo, P.; Reay-Jones, F.P.F.; Greene, J.K. First report of a mermithid nematode infecting the invasive Megacopta cribraria (Hemiptera: Plataspidae) in the United States. J. Invert. Pathol. 2015, 127, 35–37. [Google Scholar] [CrossRef]
  22. Gardner, W.A.; Blount, J.L.; Golec, J.R.; Jones, W.A.; Hu, X.P.; Talamas, E.J.; Evans, R.M.; Dong, X.; Ray, C.H., Jr.; Buntin, G.D.; et al. Discovery of Paratelenomus saccharalis (Dodd) (Hymenoptera: Platygastridae), an egg parasitoid of Megacopta cribraria F. (Hemiptera: Plataspidae) in its expanded North American Range. J. Entomol. Sci. 2013, 48, 355–359. [Google Scholar] [CrossRef]
  23. Ademokoya, B.; Balusu, R.; Ray, C.; Mottern, J.; Fadamiro, H. The First Record of Ooencyrtus nezarae (Hymenoptera: Encyrtidae) on Kudzu Bug (Hemiptera: Plataspidae) in North America. J. Insect Sci. 2018, 18, 105. [Google Scholar] [CrossRef] [Green Version]
  24. Britt, K.; Grant, J.F.; Wiggins, G.J.; Stewart, S.D. Prevalence and Localized Infection of the Entomopathogenic Fungus Beauveria bassiana on Kudzu Bug (Hemiptera: Plataspidae) in Eastern Tennessee. J. Entomol. Sci. 2016, 51, 321–324. [Google Scholar] [CrossRef]
  25. Portilla, M.; Jones, W.; Perera, O.; Seiter, N.; Greene, J.; Luttrell, R. Estimation of Median Lethal Concentration of Three Isolates of Beauveria bassiana for Control of Megacopta cribraria (Heteroptera: Plataspidae) Bioassayed on Solid Lygus spp. Diet. Insects 2016, 7, 31. [Google Scholar] [CrossRef]
  26. Seiter, N.J.; Grabke, A.; Greene, J.K.; Kerrigan, J.L.; Reay-Jones, F.P.F. Beauveria bassiana is a pathogen of Megacopta cribraria (Hemiptera: Plataspidae) in South Carolina. J. Entomol. Sci. 2014, 49, 326–330. [Google Scholar] [CrossRef]
  27. Gardner, W.; Olson, D.M. Population Census of Megacopta cribraria (Hemiptera: Plataspidae) in Kudzu in Georgia, U.S.A., 2013–2016. J. Entomol. Sci. 2016, 51, 325–328. [Google Scholar] [CrossRef]
  28. Leland, J.E.; Snodgrass, G.L. Prevalence of naturally occurring Beauveria bassiana in Lygus lineolaris (Heteroptera: Miridae) populations from wild host plants of Mississippi. J. Agric. Urban Entomol. 2005, 21, 157–163. [Google Scholar]
  29. Snodgrass, G.L.; Scott, W.P.; Robbins, J.T.; Hardee, D.D. Area-wide management of the tarnished plant bug by reduction of early-season wild host plant density. Southwest. Entomol. 2000, 23, 59–66. [Google Scholar]
  30. Parys, K.A.; Portilla, M. Effectiveness of Beauveria bassiana against Piezodorus guildinii (Hemiptera: Pentatomidae), a key pest of soybeans in the neotropics. Biocontrol Sci. Technol. 2020, 30, 451–461. [Google Scholar] [CrossRef]
  31. Portilla, M.; Abbas, H.K.; Accinelli, C.; Luttrell, R. Laboratory and field investigation on compatibility of Beauveria bassiana (Hypocreales: Clavicipotaceae) spores with a sprayable bioplastic formulation for application in the biocontrol of tarnished plant bug in cotton. J. Econ. Entomol. 2018, 20, 1–9. [Google Scholar] [CrossRef] [PubMed]
  32. Portilla, M.; Snodgrass, G.; Luttrell, R. A novel bioassay to evaluate the potential of Beauvaria bassiana strain NI8 and the insect growth regulator novaluron against Lygus lineolaris on a non-autoclaved solid artificial diet. J. Insect Sci. 2014, 14, 1–13. [Google Scholar] [CrossRef] [PubMed]
  33. SAS Institute. SAS 9.4 User’s Guide; SAS Institute: Cary, NC, USA, 2018. [Google Scholar]
  34. Abbott, W.S. A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 1925, 18, 265–267. [Google Scholar] [CrossRef]
  35. Forlani, L.; Pedrini, N.; Girotti, J.R.; Mijailovsky, S.J.; Cardozo, R.M.; Gentile, A.G.; Hernandez-Suarez, C.M.; Rabinovich, J.E.; Juarez, M.P. Biological Control of the Chagas Disease Vector Triatoma infestans with the Entomopathogenic Fungus Beauveria bassiana Combined with an Aggregation Cue: Field, Laboratory and Mathematical Modeling Assessment. PLoS Negl. Trop. Dis. 2015, 9, e0003778. [Google Scholar] [CrossRef]
  36. Goodell, P.B. Managing Lygus in the Landscape. In Proceedings of the California Chapter of the American Agronomy Society Plant and Soil Conference, Anaheim, CA, USA, 7–8 February 2001. [Google Scholar]
  37. Shapiro-Ilan, D.I.; Reilly, C.C.; Hotchkiss, M.W. Comparative impact of artificial selection for fungicide resistance on Beauveria bassiana and Metarhizium brunneum. Environ. Entomol. 2011, 40, 59–65. [Google Scholar] [CrossRef] [PubMed]
  38. Knight, I.A.; Roberts, P.M.; Gardner, W.A.; Oliver, K.M.; Jacobson, A.; Toews, M.D. Effect of Beauveria bassiana (Hypocreales: Clavicipitaceae) and Fungicide Applications on Megacopta cribraria (Hemiptera: Plataspidae) in Soybean. Crop Forage Turfgrass Manag. 2017, 3, 34. [Google Scholar] [CrossRef]
  39. Niland, E.S.; Lewallen, C.; Carrillo, C.; Perez, L.; Thompson, A.; Gardner, W.A. Quantification and Molecular Identification of Beauveria bassiana (Hypocreales: Clavicipitaceae) in Soils Associated with Megacopta cribraria (Hemiptera: Plastaspidae). J. Entomol. Sci. 2019, 54, 93–98. [Google Scholar] [CrossRef]
  40. Olson, D.M.; Tillman, G.; Toews, M. Biopesticide and Parasitoid Effects on Megacopta cribraria (Hemiptera: Plataspidae) Life Stage Density and Egg Parasitism in Soybean. J. Entomol. Sci. 2021, 56, 272–277. [Google Scholar] [CrossRef]
  41. Lahiri, S.; Reisig, D.D.; Dean, L.L.; Reay-Jones, F.P.F.; Greene, J.K.; Carter, T.E.; Mian, R.; Fallen, B.D. Mechanisms of Soybean Host-Plant Resistance Against Megacopta cribraria (Hemiptera: Plataspidae). Environ. Entomol. 2020, 49, 876–885. [Google Scholar] [CrossRef]
Pathogens 11 01028 g001 550
Figure 1. Survival estimates for second and third instar nymphs of kudzu bugs exposed to two native strains of Beauveria bassiana [NI8 (Mississippi Delta) (A,C) and KUDSC (South Carolina) (B,D)] at four concentrations (spores/mL). Survival probability at age x (p = 0.5, LIFETEST of Equality over Strata).
Figure 1. Survival estimates for second and third instar nymphs of kudzu bugs exposed to two native strains of Beauveria bassiana [NI8 (Mississippi Delta) (A,C) and KUDSC (South Carolina) (B,D)] at four concentrations (spores/mL). Survival probability at age x (p = 0.5, LIFETEST of Equality over Strata).
Pathogens 11 01028 g001
Pathogens 11 01028 g002 550
Figure 2. Product-limit survival estimates for fourth and fifth instar nymphs of kudzu bugs exposed to two native strains of Beauveria bassiana [NI8 (Mississippi Delta) and KUDSC (South Carolina)] at four concentrations (spores/mL) (A–D). Survival probability at age x (p = 0.5, LIFETEST of Equality over Strata).
Figure 2. Product-limit survival estimates for fourth and fifth instar nymphs of kudzu bugs exposed to two native strains of Beauveria bassiana [NI8 (Mississippi Delta) and KUDSC (South Carolina)] at four concentrations (spores/mL) (A–D). Survival probability at age x (p = 0.5, LIFETEST of Equality over Strata).
Pathogens 11 01028 g002
Pathogens 11 01028 g003 550
Figure 3. Product-limit survival estimates for adults of kudzu bugs exposed to two native strains of Beauveria bassiana [NI8 (Mississippi Delta) (A) and KUDSC (South Carolina) (B)] at four concentrations (spores/mL). Survival probability at age x (p = 0.5, LIFETEST of Equality over Strata).
Figure 3. Product-limit survival estimates for adults of kudzu bugs exposed to two native strains of Beauveria bassiana [NI8 (Mississippi Delta) (A) and KUDSC (South Carolina) (B)] at four concentrations (spores/mL). Survival probability at age x (p = 0.5, LIFETEST of Equality over Strata).
Pathogens 11 01028 g003
Table
Table 1. Mortality of kudzu bug nymphs and adults following exposure to different concentrations of two Beauveria bassiana native strains from South Carolina (KUDSC) and Mississippi Delta (NI8).
Table 1. Mortality of kudzu bug nymphs and adults following exposure to different concentrations of two Beauveria bassiana native strains from South Carolina (KUDSC) and Mississippi Delta (NI8).
Treatments 1
(Spores/mL)		Kudzu Bug Stages (% Mean)
2nd Instar3rd Instar4th Instar5th InstarAdult
Day 10
Control12.21 ± 3.54 de10.0 ± 3.23 d13.33 ± 3.6 c13.33 ± 3.6 d14.44 ± 3.72 d
7 × 104-NI824.4 ± 4.63 bcde36.7 ± 5.1 abc26.66 ± 4.67 bc24.44 ± 4.56 dc38.89 ± 5.17 c
7 × 105-NI827.82 ± 4.74 bcde40.0 ± 5.21 ab32.22 ± 4.95 bc31.11 ± 4.91 dc37.78 ± 5.14 c
7 × 106-NI831.12 ± 4.93 cdb53.3 ± 5.35 a43.33 ± 5.25 ab45.56 ± 5.28 abc51.11 ± 5.30 bc
7 × 107-NI856.70 ± 5.30 a55.6 ± 5.2 a56.67 ± 5.25 a57.78 ± 5.24 a68.89 ± 4.91 ab
7 × 104-KUDSC7.81 ± 2.80 e15.6 ± 3.82 cd22.22 ± 4.41 bc31.11 ± 4.91 dc76.67 ± 4.48 a
7 × 105-KUDSC20.00 ± 4.24 cde27.8 ± 4.76 bcd27.78 ± 4.75 bc26.67 ± 4.69 dc80.00 ± 4.24 a
7 × 106-KUDSC42.23 ± 5.21 ab35.6 ± 5.14 abc42.22 ± 5.24 ab38.89 ± 5.17 bc81.11 ± 4.15 a
7 × 107-KUDSC40.05 ± 5.28 abc53.3 ± 5.3 a63.33 ± 5.11 a64.44 ± 4.5 a86.67 ± 3.6 a
Day 20
Control21.12 ± 4.39 f17.80 ± 4.12 d21.11 ± 4.33 d16.67 ± 2.53 e15.56 ± 3.84 e
7 × 104-NI841.16 ± 5.25 edf46.75 ± 5.3 bc35.56 ± 5.07 cd28.89 ± 4.8 ed62.22 ± 5.14 dc
7 × 105-NI851.13 ± 5.33 edc50.03 ± 5.3 b48.89 ± 5.3 bc37.78 ± 5.14 edc56.67 ± 5.25 d
7 × 106-NI864.40 ± 5.10 abc64.41 ± 4.51 ab58.89 ± 5.22 b56.67 ± 5.25 abc70.00 ± 4.85 bcd
7 × 107-NI884.41 ± 3.83 a76.72 ± 4.8 a85.56 ± 3.73 a72.22 ± 5.28 a90.00 ± 3.18 a
7 × 104-KUDSC37.78 ± 5.17 ef25.63 ± 4.68 cd33.33 ± 4.5 cd45.56 ± 5.28 bdc80.00 ± 4.23 abc
7 × 105-KUDSC37.03 ± 5.12 ef47.83 ± 5.33 b37.78 ± 5.14 cd31.11 ± 4.9 ed81.11 ± 4.15 abc
7 × 106-KUDSC62.20 ± 5.15 edc64.40 ± 5.1 ab63.33 ± 5.11 b53.33 ± 5.29 abc81.11 ± 4.45 abc
7 × 107-KUDSC73.33 ± 4.70 ab85.65 ± 3.71 a95.56 ± 2.18 a66.67 ± 4.5 ab86.67 ± 3.6 ab
Means ± SD. Followed by the same letter in each column are not significantly different (p < 0.05 Tukey test).
Table
Table 2. Lethal mortality-response (LC50) of different stages of kudzu bugs treated with Beauveria bassiana, Mississippi Delta native NI8 strains.
Table 2. Lethal mortality-response (LC50) of different stages of kudzu bugs treated with Beauveria bassiana, Mississippi Delta native NI8 strains.
StageConcentration Response (Spores/mm2)
nSlope ± SELC50 (95% CI)Probit TrendRR50 (95% CI) *
Test for SlopeTest for GoF
X2p > X2X2p > X2
10 Days
2nd Instar4500.18 ± 0.07789 (77–3.1 × 1026)5.30.02141.890.041517 (1–309)
3rd Instar4500.09 ± 0.03199 (24–7.3 × 104)8.510.00350.680.74363 (0.13–50)
4th Instar4500.15 ± 0.04480 (101–1.0 × 104)14.180.00021.10.35518 (0.61–110)
5th Instar4500.18 ± 0.04307 (85–2.4 ×103)17.290.00011.440.92656 (0.57–73)
Adult4500.15 ± 0.0473 (5–4.5 × 103)9.30.00231.750.05681
20 Days
2nd Instar4500.22 ± 0.0413 (3–36)28.650.00011.220.268016 (0.46–588)
3rd Instar4500.15 ± 0.0311 (2–45)18.460.00011.480.226510 (0.22–429)
4th Instar4500.25 ± 0.0520 (6–51)28.030.00011.230.262432 (0.94–1096)
5th Instar4500.17 ± 0.03133 (33–879)18.970.00011.240.2532183 (5–6494)
Adult4500.54 ± 0.051 (0.0005–6)10.090.00151.930.03631
* Differences among RR50 values are significant if 95% CI does not include 1.0. RR50 compared to LC50s among the lowest LC50 as a control.
Table
Table 3. Lethal mortality–response (LC50) of Kudzu bugs treated with Beauveria bassiana, South Carolina native KUDSC strain.
Table 3. Lethal mortality–response (LC50) of Kudzu bugs treated with Beauveria bassiana, South Carolina native KUDSC strain.
StageConcentration Response (Spores/mm2)
nSlope ± SELC50 (95% CI)Probit TrendRR50 (95% CI) *
Test for SlopeTest for GoF
X2p > X2X2p > X2
10 Days
2nd Instar4500.21 ± 0.041051 (286–12,053)22.540.00011.520.1249438 (71–2694)
3rd Instar4500.19 ± 0.04464 (140–3705)19.930.00011.040.4043184 (35–981)
4th Instar4500.23 ± 0.05205 (70–864)17.420.00011.110.346267 (15–360)
5th Instar4500.22 ± 0.05182 (61–748)19.560.00010.830.593158 (13–271)
Adult4500.23 ± 0.033 (1–7)47.510.00011.310.21591
20 Days
2nd Instar4500.21 ± 0.0449 (12–164)19.660.00010.670.757325 (1.32–482)
3rd Instar4500.30 ± 0.0417 (7–36)44.110.00010.840.588919 (1.26–284)
4th Instar4500.52 ± 0.1528 (2–65)11.280.00081.840.047932 (1.74–580)
5th Instar4500.21 ± 0.0482 (27–297)25.380.00011.510.126768 (4–1117)
Adult4500.21 ± 0.061.14 (0.006–7)11.620.00073.220.00041
* Differences among RR50 values are significant if 95% CI do not include 1.0. RR50 compare the LC50s among the lowest LC50 as a control.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Glover, J.P.; Portilla, M.; Parys, K.; Allen, C.; George, J.; Reddy, G.V.P. Susceptibility of Different Life Stages of Kudzu Bug Megacopta cribraria (F.) (Hemiptera: Plataspidae) to Two Different Native Strains of Beauveria bassiana. Pathogens 2022, 11, 1028. https://doi.org/10.3390/pathogens11091028

AMA Style

Glover JP, Portilla M, Parys K, Allen C, George J, Reddy GVP. Susceptibility of Different Life Stages of Kudzu Bug Megacopta cribraria (F.) (Hemiptera: Plataspidae) to Two Different Native Strains of Beauveria bassiana. Pathogens. 2022; 11(9):1028. https://doi.org/10.3390/pathogens11091028

Chicago/Turabian Style

Glover, James Paul, Maribel Portilla, Katherine Parys, Clint Allen, Justin George, and Gadi V. P. Reddy. 2022. "Susceptibility of Different Life Stages of Kudzu Bug Megacopta cribraria (F.) (Hemiptera: Plataspidae) to Two Different Native Strains of Beauveria bassiana" Pathogens 11, no. 9: 1028. https://doi.org/10.3390/pathogens11091028

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop