Assessment of the Role of Free-Living and Farmed Fallow Deer (Dama dama) as A Potential Source of Human Infection with Multiple-Drug-Resistant Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Strain Isolation and Molecular Confirmation
2.3. High-Resolution Melting Analysis, Sequencing and Phylogenetic Analysis
2.4. Bioserotyping Analyses
2.5. Antimicrobial Sensitivity Analysis
3. Results
3.1. Strain Isolation and Molecular Confirmation
3.2. High-Resolution Melting, Sequencing and Phylogenetic Analysis
3.3. Bioserotyping Analysis
3.4. Antimicrobial Sensitivity Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequence | Product Size | Reference |
---|---|---|---|
ail | 5’TGGTTATGCGCAAAGCCATGT3’ 5’TGGAAGTGGGTTGAATTGCA3’ | 356 bp | [39] |
ystA | 5’GTCTTCATTTGGAGGATTCGGC3’ 5’AATCACTACTGACTTCGGCTGG3’ | 134 bp | [40] |
ystB | 5’TGTCAGCATTTATTCTCAACT3’ 5’GCCGATAATGTATCATCAAG3’ | 180 bp | [40] |
ystC | 5’TCGACAAGTGAGTGACGGAG3’ 5’CCCTTACTCGCGACGAAATA3’ | 284 bp | [40] |
inv | 5’GCAGAATTCGGATACCCAGCACCATGACT3’ 5’GCAGGATCCAGCCATGAACATTCCACA3’ | 689 bp | Skurnik * |
wzz | 5’GGTGATGAGCAAGTTCAAG3’ 5’GCTAAATCCACTGCTCGCTG3’ | 418 bp | [35] |
Source | Strain Number | Virulence Markers | Genotype | Biotype | Serotype | |||
---|---|---|---|---|---|---|---|---|
ail | ystA | ystB | ystC | |||||
Free-living fallow deer | 2D ITC | − | − | + | − | G3 | 1A | NT |
2D PSB | − | − | + | − | G3 | 1A | NT | |
6D ITC | − | − | + | − | G3 | 1A | NT | |
30D ITC | − | − | + | − | G3 | 1A | NT | |
31D ITC | − | − | + | − | G3 | 1A | O:9 | |
57D ITC | − | − | + | − | G1 | 1A | O:5 | |
60D ITC | − | − | + | − | G3 | 1A | NT | |
Farmed fallow deer | 22 ITC | − | − | + | − | G1 | 1A | NT |
39 ITC | − | − | + | − | G1 | 1A | NT |
Source | Sample Number | Amoxicillin/Clavulanic Acid | Ampicillin | Cefalexin | Cefotaxime | Ceftazidime | Chloramphenicol | Ciprofloxacin | Gentamycin | Kanamycin | Nalidixic Acid | Streptomycin | Trimethoprim/Sulfamethoxazole | Tetracycline |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Free-living fallow deer | 2D ITC | R | R | R | R | R | R | I | R | R | I | R | I | I |
2D PSB | R | R | R | I | I | I | S | R | R | I | R | I | I | |
6D ITC | R | R | R | I | I | I | S | R | R | I | R | I | I | |
30D ITC | R | R | R | I | I | I | I | R | R | I | R | I | I | |
31D ITC | R | R | R | I | I | I | I | R | I | I | R | S | I | |
57D ITC | R | R | R | I | I | I | S | I | I | S | R | S | I | |
60D ITC | R | R | R | I | I | I | I | I | I | I | R | S | I | |
Farmed fallow deer | 22 ITC | R | R | R | I | I | I | S | R | R | I | R | I | I |
39 ITC | R | R | R | I | I | I | S | R | R | I | R | I | I |
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Odyniec, M.; Bancerz-Kisiel, A. Assessment of the Role of Free-Living and Farmed Fallow Deer (Dama dama) as A Potential Source of Human Infection with Multiple-Drug-Resistant Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis. Pathogens 2022, 11, 1266. https://doi.org/10.3390/pathogens11111266
Odyniec M, Bancerz-Kisiel A. Assessment of the Role of Free-Living and Farmed Fallow Deer (Dama dama) as A Potential Source of Human Infection with Multiple-Drug-Resistant Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis. Pathogens. 2022; 11(11):1266. https://doi.org/10.3390/pathogens11111266
Chicago/Turabian StyleOdyniec, Marta, and Agata Bancerz-Kisiel. 2022. "Assessment of the Role of Free-Living and Farmed Fallow Deer (Dama dama) as A Potential Source of Human Infection with Multiple-Drug-Resistant Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis" Pathogens 11, no. 11: 1266. https://doi.org/10.3390/pathogens11111266