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Brief Report
Peer-Review Record

In Vitro Assessment of Berberine against Ichthyophthirius multifiliis in Goldfish

Pathogens 2022, 11(10), 1207; https://doi.org/10.3390/pathogens11101207
by Ke Huang 1,2, Guangran Hu 1,2, Runqiu Wang 3, Qingwen Zeng 1,2, Wenxiang Li 1, Hong Zou 1, Shangong Wu 1, Guitang Wang 1 and Ming Li 1,*
Reviewer 1:
Pathogens 2022, 11(10), 1207; https://doi.org/10.3390/pathogens11101207
Submission received: 25 September 2022 / Revised: 16 October 2022 / Accepted: 18 October 2022 / Published: 20 October 2022

Round 1

Reviewer 1 Report

Abstract: line 14 instead free-living periods say free-living life stages.

 

Introduction Line 28: Do you mean low host-specificity?


Methods: Line 67: are these the same goldfish as in section 2.1?

Line 73: change to Macklin (Shanghai, China)

Line 78 in vitro italicised.

 

Results: Line 162 is there mortality at 300 mg/L?

Line 168: in vitro italicised

 

 

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

The article is interesting. The authors describe the effect of berberine against the parasite Ichthyophthirius multifiliis. A very important observation is that berberine at 15 mg/L changes the shape of protomonts, destroys their organelles, and decreases the number of ribosomes.

I suggest some corrections:

1. In the Materials and Methods should be more details, including the composition of the feed, pH of the water. Why was used distilled water, instead of 0.9% NaCl solution or the addition of horse serum?

2. Please add in the Introduction, the figure with a life cycle of Ichthyophthirius multifiliis, for better understanding by readers not knowing this parasite.

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

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