In Vitro Assessment of Berberine against Ichthyophthirius multifiliis in Goldfish
Round 1
Reviewer 1 Report
Abstract: line 14 instead free-living periods say free-living life stages.
Introduction Line 28: Do you mean low host-specificity?
Methods: Line 67: are these the same goldfish as in section 2.1?
Line 73: change to Macklin (Shanghai, China)
Line 78 in vitro italicised.
Results: Line 162 is there mortality at 300 mg/L?
Line 168: in vitro italicised
Author Response
Please see the attachment.
Author Response File: Author Response.docx
Reviewer 2 Report
The article is interesting. The authors describe the effect of berberine against the parasite Ichthyophthirius multifiliis. A very important observation is that berberine at 15 mg/L changes the shape of protomonts, destroys their organelles, and decreases the number of ribosomes.
I suggest some corrections:
1. In the Materials and Methods should be more details, including the composition of the feed, pH of the water. Why was used distilled water, instead of 0.9% NaCl solution or the addition of horse serum?
2. Please add in the Introduction, the figure with a life cycle of Ichthyophthirius multifiliis, for better understanding by readers not knowing this parasite.
Author Response
Please see the attachment.
Author Response File: Author Response.docx