Drug Resistance and Molecular Characteristics of Mycobacterium tuberculosis: A Single Center Experience
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimen Collection
2.2. Drug Resistance Pattern
2.3. Principle of PCR-Reverse Membrane Hybridization
2.4. Mycobacterium Species Identification
2.5. Detection of Mutation in MTB Drug Resistance-Related Gene
2.6. Histological Staining
2.7. Statistical Analysis
3. Results
3.1. Histological Features
3.2. Drug Resistance and Molecular Characteristics of MTB
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | MTB-Positive FFPE Specimens | |
---|---|---|
Age | 43.00 (29) | |
≥60/<60 | 68.00 (10)/36.00 (21) | |
Gender | male/female | 211/169 |
Tissue type | lung/kidney/liver/intestine/brain/thyroid | 39/57/2/8/3/1 |
spine/arthrosis | 172/45 | |
lymph node | 13 | |
skin | 2 | |
pericardium/pleura/peritoneum | 2/25/11 | |
Treatment type | new cases/previously treated cases | 281/99 |
Region | the north of China/the southeast of China | 278/36 |
the Central and western of China | 66 |
Drug Resistance | New Cases | Previously Treated | Total | χ2 | p |
---|---|---|---|---|---|
n = 281 | Cases (n = 99) | n = 380 | |||
n, % | n, % | n, % | |||
Susceptible | 68, 24.20 | 17, 17.17 | 85, 22.37 | 2.082 | 0.149 |
Any resistant | 213, 75.80 | 82, 82.83 | 295, 77.63 | 2.082 | 0.149 |
Monodrug-resistant | 74, 26.33 | 19, 19.19 | 93, 24.47 | 2.021 | 0.155 |
resistance to RIF | 3, 1.07 | 1, 1.01 | 4, 1.05 | ||
resistance to INH | 65, 23.13 | 18, 18.18 | 83, 21.84 | ||
resistance to STR | 4, 1.42 | 0, 0 | 4, 1.05 | ||
resistance to EMB | 2, 0.71 | 0, 0 | 2, 0.53 | ||
MDR | 103, 36.65 | 52, 52.53 | 155, 40.80 | 7.635 | 0.006 * |
resistance to RIF + INH | 32, 11.39 | 12, 12.12 | 4, 11.58 | ||
resistance to RIF + INH + STR | 22, 7.83 | 15, 15.15 | 37, 9.74 | ||
resistance to RIF + INH + EMB | 15, 5.34 | 3, 3.03 | 18, 4.74 | ||
resistance to RIF + INH + STR +EMB | 34, 12.10 | 22, 22.22 | 56, 14.74 | ||
Polydrug-resistant | 36, 12.81 | 11, 11.11 | 47, 12.37 | 0.021 | 0.884 |
resistance to RIF + STR | 2, 0.71 | 0, 0 | 2, 0.53 | ||
resistance to RIF + EMB | 1, 0.36 | 0, 0 | 1, 0.26 | ||
resistance to RIF + STR +EMB | 1, 0.36 | 0, 0 | 1, 0.26 | ||
resistance to INH + STR | 12, 4.27 | 4, 4.04 | 16, 4.21 | ||
resistance to INH + EMB | 7, 2.49 | 5, 5.05 | 12, 3.16 | ||
resistance to INH + STR + EMB | 11, 3.91 | 2, 2.02 | 13, 3.42 | ||
resistance to STR + EMB | 2, 0.71 | 0, 0 | 2, 0.53 |
Parameter | Males | Females | χ2 | p | |
---|---|---|---|---|---|
New cases | Susceptible | 36 | 32 | 0.179 | 0.673 |
Any resistance | 119 | 94 | |||
Previously treated cases | Susceptible | 5 | 12 | 6.610 | 0.013 * |
Any resistance | 51 | 31 | |||
χ2 | - | 5.371 | 0.105 | - | - |
p | - | 0.020 * | 0.746 | - | - |
Drug and Gene | Mutation Form | Total (n = 380) | |
---|---|---|---|
n | % | ||
RIF, rpoB | D516V | 53 | 13.95 |
D516G | 2 | 0.53 | |
H526Y | 1 | 0.26 | |
S531L | 6 | 1.58 | |
D516V + D516G | 24 | 6.32 | |
D516V + H526Y | 3 | 0.79 | |
D516V + H526D | 11 | 2.89 | |
D516V + S531L | 1 | 0.26 | |
D516V + D516G + H526D | 17 | 4.47 | |
D516V + D516G + S531L | 1 | 0.26 | |
D516V + D516G + H526Y + H526D | 9 | 2.37 | |
D516V + D516G + H526Y + H526D + S531L | 8 | 2.11 | |
D516V + D516G + H526Y + H526D + S531L + S531W | 7 | 1.84 | |
D516V + D516G + H526D + S531L | 5 | 1.32 | |
D516V + H526Y + H526D | 2 | 0.53 | |
D516V + H526Y + H526D + S531L | 1 | 0.26 | |
D516V + H526D + S531L | 3 | 0.79 | |
D516G + H526Y | 1 | 0.26 | |
D516G + H526D | 2 | 0.53 | |
H526Y + H526D | 3 | 0.79 | |
H526Y + H526D + S531L | 1 | 0.26 | |
H526D + S531L | 2 | 0.53 | |
INH, katG | 315 M | 219 | 57.63 |
inhA | −15 M | 9 | 2.37 |
−15 M + 315 M | 51 | 13.42 | |
43 M | 28 | 7.37 | |
STR, rpsL | 88 M | 71 | 18.68 |
43 M + 88 M | 32 | 8.42 | |
306 M1 | 6 | 1.58 | |
306 M2 | 75 | 19.74 | |
EMB, embB | 306 M1 + 306 M2 | 18 | 4.74 |
306 M2 + 306 M3 | 1 | 0.26 | |
306 M1 + 306 M2 + 306 M3 | 5 | 1.32 |
Drug and Total Case of Resistace | Mutation Sites | Mutation in Monodrug-Resistant | Mutation in Resistance to More than One Drug | χ2 | p |
---|---|---|---|---|---|
n | n, % | n, % | |||
RIF, 163 | D516V | 4, 2.45 | 141, 86.5 | 0.509 | 0.476 |
D516G | 1, 0.61 | 75, 46.01 | 0.771 | 0.380 | |
H526Y | 1, 0.61 | 35, 21.47 | 0.020 | 0.887 | |
H526D | 0, 0 | 71, 43.56 | 3.165 | 0.075 | |
S531L | 0, 0 | 35, 21.47 | 1.121 | 0.290 | |
S531W | 0, 0 | 7, 4.29 | 0.184 | 0.668 | |
INH, 279 | 315 M | 81, 29.03 | 189, 67.74 | 0.252 | 0.616 |
−15 M | 5, 1.79 | 55, 19.71 | 16.775 | 0.000 * | |
STR, 131 | 43 M | 2, 1.53 | 58, 44.27 | 0.029 | 0.864 |
88 M | 2, 1.53 | 101, 77.10 | 2.012 | 0.156 | |
306 M1 | 0, 0 | 29, 27.62 | 0.778 | 0.378 | |
EMB, 105 | 306 M2 | 2, 1.90 | 97, 92.38 | 0.124 | 0.725 |
306 M3 | 0, 0 | 6, 5.71 | 0.124 | 0.725 |
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Li, S.; Chen, W.; Feng, M.; Liu, Y.; Wang, F. Drug Resistance and Molecular Characteristics of Mycobacterium tuberculosis: A Single Center Experience. J. Pers. Med. 2022, 12, 2088. https://doi.org/10.3390/jpm12122088
Li S, Chen W, Feng M, Liu Y, Wang F. Drug Resistance and Molecular Characteristics of Mycobacterium tuberculosis: A Single Center Experience. Journal of Personalized Medicine. 2022; 12(12):2088. https://doi.org/10.3390/jpm12122088
Chicago/Turabian StyleLi, Shanshan, Wen Chen, Mengru Feng, Yuejiao Liu, and Fenghua Wang. 2022. "Drug Resistance and Molecular Characteristics of Mycobacterium tuberculosis: A Single Center Experience" Journal of Personalized Medicine 12, no. 12: 2088. https://doi.org/10.3390/jpm12122088