Devascularized Bone Surface Culture: A Novel Strategy for Identifying Osteomyelitis-Related Pathogens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Setting, OM Diagnostic Criteria, and Inclusion and Exclusion Criteria
2.2. BSC and TSC Procedures
2.3. Statistical Analysis
3. Results
3.1. Participant Inclusion Flow Chart, Patient Demographics, OM Etiology, Body Side, and Infection Site Distributions
3.2. BSC and TSC Outcomes and Culture Time
3.3. Microorganism Type
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Case No. | Sex/Age (Year) | Infection Site | BSC Outcome | BSC Time (Day) | TSC Outcome | TSC Time (Day) |
---|---|---|---|---|---|---|
1 | M/14 | Femur | Staphylococcus aureus | 1 | Staphylococcus aureus | 3 |
2 | M/29 | Tibia | Staphylococcus aureus | 1 | Staphylococcus aureus | 3 |
3 | M/41 | Tibia | Achromobacter xylosoxidans + Acinetobacter lwoffii | 3 | Negative | NA |
4 | M/49 | Tibia | Escherichia coli | 1 | Escherichia coli | 3 |
5 | F/49 | Calcaneus | Negative | NA | Negative | NA |
6 | M/44 | Tibia | Staphylococcus epidermidis | 3 | Negative | NA |
7 | M/53 | Femur | Staphylococcus aureus | 1 | Staphylococcus aureus | 3 |
8 | M/14 | Tibia | Streptococcus pyogenes | 1 | Streptococcus pyogenes | 2 |
9 | F/32 | Tibia | Candida parapsilosis | 1 | Candida parapsilosis | 2 |
10 | M/71 | Tibia | Proteus mirabilis | 1 | Proteus mirabilis | 2 |
11 | M/59 | Femur | Proteus mirabilis | 1 | Proteus mirabilis | 2 |
12 | M/10 | Phalange | Staphylococcus epidermidis | 1 | Staphylococcus epidermidis | 3 |
13 | M/53 | Calcaneus | Proteus mirabilis + Staphylococcus felis | 1 | Proteus mirabilis + Staphylococcus felis | 5 |
14 | M/51 | Calcaneus | Staphylococcus aureus | 1 | Staphylococcus aureus | 4 |
15 | M/59 | Radius | Staphylococcus aureus | 1 | Negative | NA |
16 | M/21 | Humerus | Negative | NA | Negative | NA |
17 | M/50 | Tibia | Staphylococcus aureus | 1 | Staphylococcus aureus | 2 |
18 | M/49 | Tibia | Staphylococcus aureus | 1 | Staphylococcus aureus | 4 |
19 | M/16 | Femur | Enterococcus faecalis | 1 | Negative | NA |
20 | F/68 | Tibia | Negative | NA | Negative | NA |
21 | M/68 | Calcaneus | Proteus mirabilis | 1 | Proteus mirabilis | 3 |
22 | F/54 | Calcaneus | Negative | NA | Negative | NA |
23 | M/46 | Calcaneus | Staphylococcus aureus | 1 | Staphylococcus aureus | 3 |
24 | F/48 | Femur | Staphylococcus aureus | 1 | Staphylococcus aureus | 4 |
25 | M/42 | Tibia | Enterobacter cloacae | 1 | Negative | NA |
26 | M/37 | Tibia | Negative | NA | Negative | NA |
27 | M/39 | Tibia | Mycobacterium fortuitum | 3 | Mycobacterium fortuitum | 3 |
28 | M/87 | Humerus | Pseudomonas aeruginosa | 3 | Pseudomonas aeruginosa | 3 |
29 | M/52 | Calcaneus | Negative | NA | Negative | NA |
30 | M/47 | Calcaneus | Staphylococcus aureus | 1 | Staphylococcus aureus | 3 |
31 | M/15 | Tibia | Negative | NA | Negative | NA |
32 | M/29 | Phalange | Staphylococcus aureus | 1 | Staphylococcus aureus | 2 |
33 | M/36 | Femur | Negative | NA | Staphylococcus aureus | 3 |
34 | M/52 | Tibia | Pseudomonas aeruginosa | 3 | Negative | NA |
35 | M/56 | Tibia | Staphylococcus warneri | 2 | Staphylococcus warneri | 3 |
36 | M/32 | Tibia | Negative | NA | Negative | NA |
37 | M/67 | Calcaneus | Escherichia coli | 1 | Escherichia coli | 4 |
38 | M/29 | Tibia | Staphylococcus haemolyticus | 1 | Staphylococcus haemolyticus | 3 |
39 | M/45 | Calcaneus | Negative | NA | Negative | NA |
40 | M/37 | Tibia | Negative | NA | Negative | NA |
41 | M/49 | Ulna | Serratia marcescens | 1 | Negative | NA |
42 | M/66 | Tibia | Staphylococcus aureus | 1 | Staphylococcus aureus | 2 |
43 | M/25 | Tibia | Staphylococcus aureus | 1 | Staphylococcus aureus | 3 |
44 | M/48 | Calcaneus | Streptococcus agalactiae | 1 | Streptococcus agalactiae | 4 |
45 | F/63 | Tibia | Streptococcus dysgalactiae | 2 | Streptococcus dysgalactiae | 3 |
46 | M/30 | Tibia | Proteus mirabilis | 1 | Negative | NA |
47 | M/43 | Femur | Enterobacter aerogenes | 1 | Enterobacter aerogenes | 3 |
48 | M/49 | Tibia | Negative | NA | Negative | NA |
49 | F/8 | Tibia | Negative | NA | Negative | NA |
50 | M/30 | Femur | Escherichia coli + Enterococcus | 1 | Escherichia coli + Enterococcus | 2 |
51 | F/65 | Humerus | Enterobacter asburiae + Enterococcus faecalis | 2 | Negative | NA |
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Chen, P.; Lin, Q.-r.; Huang, M.-Z.; Zhang, X.; Hu, Y.-j.; Chen, J.; Jiang, N.; Yu, B. Devascularized Bone Surface Culture: A Novel Strategy for Identifying Osteomyelitis-Related Pathogens. J. Pers. Med. 2022, 12, 2050. https://doi.org/10.3390/jpm12122050
Chen P, Lin Q-r, Huang M-Z, Zhang X, Hu Y-j, Chen J, Jiang N, Yu B. Devascularized Bone Surface Culture: A Novel Strategy for Identifying Osteomyelitis-Related Pathogens. Journal of Personalized Medicine. 2022; 12(12):2050. https://doi.org/10.3390/jpm12122050
Chicago/Turabian StyleChen, Peng, Qing-rong Lin, Mou-Zhang Huang, Xin Zhang, Yan-jun Hu, Jing Chen, Nan Jiang, and Bin Yu. 2022. "Devascularized Bone Surface Culture: A Novel Strategy for Identifying Osteomyelitis-Related Pathogens" Journal of Personalized Medicine 12, no. 12: 2050. https://doi.org/10.3390/jpm12122050