Integrated Analysis of Tumor Mutation Burden and Immune Infiltrates in Hepatocellular Carcinoma
Round 1
Reviewer 1 Report
Manuscript entitled “Integrated Analysis of Tumor Mutation Burden and Immune Infiltrates in Hepatocellular Carcinoma” by Yulan Zhao et al reports the association of tumor mutation burden (TMB) and responsiveness to immunotherapy of hepatocellular carcinoma. Neoantigens or neoepitopes produced as result of tumor mutation plays an important role in immunotherapy of cancer. Development of immunotherapeutic approaches targeting this neoantigens may change the landscape of cancer immunotherapy. Authors have shown the direct association of NCBP2, one of the differentially expressed genes and poor prognosis of HCC in the context of infiltration of various immune cell subsets into the tumor microenvironment. This study has been well conducted using multiple bio-informatic tools and warrants publication in diagnostics.
Reviewer 2 Report
I appreciated very much Your work, the issue raised in the field of HCC , the sharp experimental design, the very clear way to report the bioinformatic, the novel informations obtained with clinical value. The authors use a rigorous way to interpret and propose their results. In the field of HCC they present NCBP2, a cap-binding pre-mRNA protein, as a DE mRNA with clinical value. Other authors had previuosly shown its overexpression in diverse cancer types. This molecule seems to play a relevant role in Tumor progression of diverse cancer types.
