Comparative Transcriptome Analysis of Eriocheir sinensis from Wild Habitats in Han River, Korea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Species Identification
2.3. RNA Extraction
2.4. cDNA Library and High Throughput Sequencing
2.5. De Novo Transcriptome Assembly
2.6. Assembly Assessment and Unigene Calculation
2.7. Functional Annotation of Unigenes
2.8. Differential Gene Expression Analysis
2.9. Network Analysis and Community Detection
3. Results and Discussion
3.1. RNA Sequencing and De Novo Assembly
3.2. Unigene Functional Annotation
3.3. DEGs and Functional Analysis
3.4. GO and KEGG Enrichment Analysis of DEGs
3.5. Interaction Network of DEGs
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Description | Statistics |
---|---|
Number of transcripts | 269,883 |
Percent GC content (%) | 45.4 |
Contig N50 | 1732 |
Median contig length (bp) | 476 |
Average contig length (bp) | 952.39 |
Total assembled bases (bp) | 257,034,330 |
Total number of unigenes | 214,590 |
Percent unigenes GC content (%) | 45.1 |
Longest unigene (bp) | 29,539 |
Shortest unigene (bp) | 181 |
Description | Number of Unigenes | Percentage (%) |
---|---|---|
Total number of longest ORF unigene | 138,261 | |
Annotated in Swiss-Prot | 31,377 | 22.69 |
Annotated in GO | 1277 | 0.92 |
Annotated in Pfam | 31,072 | 22.47 |
Annotated in KEGG | 1289 | 0.93 |
Total of annotated unigene | 35,014 |
Regulation | Pathway Name | Pathway ID | Corrected p-Value |
---|---|---|---|
Up | Protein processing in endoplasmic reticulum | dme04141 | 0.01037 |
Down | Phenylalanine, tyrosine, and tryptophan biosynthesis | dme00400 | 0.01577 |
Phenylalanine metabolism | dme00360 | 0.01577 | |
Tyrosine metabolism | dme00350 | 0.02671 | |
Folate biosynthesis | dme00790 | 0.03214 | |
Biosynthesis of amino acids | dme01230 | 0.03738 | |
Longevity regulating pathway—multiple species | dme04213 | 0.03738 | |
mRNA surveillance pathway | dme03015 | 0.03738 | |
Spliceosome | dme03040 | 0.04312 | |
Protein processing in endoplasmic reticulum | dme04141 | 0.04312 | |
RNA transport | dme03013 | 0.04312 | |
Metabolic pathways | dme01100 | 0.04312 |
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An, H.-E.; Choi, T.-J.; Kim, C.-B. Comparative Transcriptome Analysis of Eriocheir sinensis from Wild Habitats in Han River, Korea. Life 2022, 12, 2027. https://doi.org/10.3390/life12122027
An H-E, Choi T-J, Kim C-B. Comparative Transcriptome Analysis of Eriocheir sinensis from Wild Habitats in Han River, Korea. Life. 2022; 12(12):2027. https://doi.org/10.3390/life12122027
Chicago/Turabian StyleAn, Hyung-Eun, Tae-June Choi, and Chang-Bae Kim. 2022. "Comparative Transcriptome Analysis of Eriocheir sinensis from Wild Habitats in Han River, Korea" Life 12, no. 12: 2027. https://doi.org/10.3390/life12122027