Analysis of Rac/Rop Small GTPase Family Expression in Santalum album L. and Their Potential Roles in Drought Stress and Hormone Treatments
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. Genome-Wide Identification and Sequence Analysis of Rac Genes in S. album
2.3. Analysis of Physicochemical Properties of SaRac
2.4. Phylogenetic Analysis
2.5. Chromosome Location and Gene Structure Analysis
2.6. Collinearity of the SaRac Gene
2.7. Expression Profiles of SaRac Genes in Different Tissues and under Drought and Hormone Treatments
3. Results
3.1. Sequence Analysis of the SaRac Gene Family in S. album
3.2. Analysis of SaRac Protein Homology
3.3. Sequence Analysis of the SaRac Gene Family
3.4. Chromosome Distribution of the SaRac Gene Family
3.5. Phylogenetic Comparison of Rac in Different Species
3.6. Analysis of Rac/Rop Multibase Regions in S. album, A. thaliana and O. sativa
3.7. Tissue Specificity of Rac Expression in S. album
3.8. Expression of SaRac Genes under Drought and Hormones Treatments
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Name | Number of Amino Acids | Molecular Weight (kDa) | PI | Instability Index | Total Number of Negatively Charged Residues (Asp + Glu) | Total Number of Positively Charged Residues (Arg + Lys) | Grand Average of Hydropathicity (GRAVY) | In Silico Prediction WOLF PSORT |
---|---|---|---|---|---|---|---|---|---|
Sal10G07090.1 | SaRac1 | 197 | 21.58 | 9.20 | 38.58 | 18 | 25 | −0.075 | plas |
Sal10G04200.1 | SaRac2 | 198 | 21.83 | 9.43 | 34.09 | 16 | 27 | −0.129 | chlo |
Sal8G02490.1 | SaRac3 | 198 | 21.81 | 9.38 | 40.23 | 18 | 25 | −0.042 | chlo |
Sal7G05910.1 | SaRac4 | 197 | 21.55 | 9.32 | 41.48 | 18 | 27 | −0.106 | chlo |
Sal9G31620.1 | SaRac5 | 196 | 21.45 | 9.25 | 39.09 | 16 | 28 | −0.047 | chlo |
Sal9G09920.1 | SaRac6 | 196 | 21.74 | 9.62 | 38.96 | 17 | 25 | −0.083 | chlo |
Sal6G04230.1 | SaRac7 | 211 | 23.28 | 9.18 | 44.44 | 19 | 28 | −0.069 | chlo |
Sal9G07020.1 | SaRac8 | 209 | 22.94 | 9.27 | 36.73 | 19 | 27 | −0.141 | plas |
Sal9G04490.1 | SaRac9 | 196 | 21.63 | 9.55 | 40.28 | 18 | 27 | −0.120 | cyto |
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Chen, Y.; Wang, S.; Liu, X.; Wang, D.; Liu, Y.; Hu, L.; Meng, S. Analysis of Rac/Rop Small GTPase Family Expression in Santalum album L. and Their Potential Roles in Drought Stress and Hormone Treatments. Life 2022, 12, 1980. https://doi.org/10.3390/life12121980
Chen Y, Wang S, Liu X, Wang D, Liu Y, Hu L, Meng S. Analysis of Rac/Rop Small GTPase Family Expression in Santalum album L. and Their Potential Roles in Drought Stress and Hormone Treatments. Life. 2022; 12(12):1980. https://doi.org/10.3390/life12121980
Chicago/Turabian StyleChen, Yu, Shengkun Wang, Xiaojing Liu, Dongli Wang, Yunshan Liu, Lipan Hu, and Sen Meng. 2022. "Analysis of Rac/Rop Small GTPase Family Expression in Santalum album L. and Their Potential Roles in Drought Stress and Hormone Treatments" Life 12, no. 12: 1980. https://doi.org/10.3390/life12121980