Identification of the EdcR Estrogen-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Strains and Growth Media
2.3. DNA Manipulation
2.4. RNA Manipulation
2.5. Construction of C. tardaugens Knockout Strains
2.6. Complementation of C. tardaugens Knockout Strains
2.7. Fluorescence Measurement of Cultures on a Plate Reader
2.8. In Silico Genomic Analysis
3. Results
3.1. In Silico Analysis of the Main Promoters Responsible for the Expression of C. tardaugens Edc Gene Cluster
3.2. Analysis of the EdcR Function
3.3. Analysis of the EdcR Effectors
3.4. Analysis of the Promoters of the Edc Cluster in E. coli. Construction of an Estrogen Biosensor
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strains | Genotype and Characteristics | Source/Reference |
---|---|---|
C. tardaugens | ||
NBRC 16725 | wild type strain (ARI-1) | [20] |
RfR | RfR strain efficient for conjugation | [22] |
ΔedcA | C. tardaugens NBRC 16725 ΔEGO55_13525 | [23] |
ΔedcR | C. tardaugens NBRC 16725 ΔEGO55_13520 | This study |
E. coli | ||
DH10B | F−, mcrA, Δ(mrr hsdRMS-mcrBC), Φ80dlacZΔM15, ΔlacX74, deoR, recA1, araD139, Δ(ara-leu)7697, galU, galK, λ−, rpsL, endA1, nupG | Invitrogen |
HB101 | supE44, ara14, galK2, leuB, lacY1, ∆(gpt-proA)62, rpsL20, xyl-5, mtl-1, recA13, ∆(mcrC-mrr), hsdS20 (rB−mB−), SmR | [27] |
Plasmids | ||
pK18mobsacB | KmR, ColE oriV, Mob+, lacZα, sacB; vector for allelic exchange homologous recombination mutagenesis | [28] |
pK18edcR | pK18mobsacB derivative used for EGO55_13520 deletion | This study |
pSEVA237PlexA | KmR, oriV (pBBR1), constituve expression of gfp gene under the control of the PlexA promoter | [29] |
pSEVA23PlexA | pSEVA237PlexA where gfp was deleted using XbaI-SpeI restriction enzymes and served as empty vector | [23] |
pSEVA23edcR | pSEVA237PlexA where gfp gene was replaced by EGO55_13520 | This study |
pSEVA237Pa | pSEVA237PlexA where PlexA promoter was replaced by Pa | This study |
pSEVA237Pb | pSEVA237PlexA where PlexA promoter was replaced by Pb | This study |
pSEVA237Pt | pSEVA237PlexA where PlexA promoter was replaced by Pt | This study |
pSEVA237M-BCD2-14g | KmR, pSEVA237M derivative, synthetic bicistronic RBS BCD2, P14g promoter | Kindly provided by P. Nikel |
pSEVA237MPb | KmR, pSEVA237M-BCD2-14g derivative where P14g promoter was replaced by Pb | This study |
pSEVA651 | GmR, oriV (RSF1010), standard MCS | [30] |
pSEVA65edcR | pSEVA651 containing EGO55_13520 gene expressed constitutively under PlexA promoter | This study |
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Ibero, J.; Galán, B.; García, J.L. Identification of the EdcR Estrogen-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor. Genes 2021, 12, 1846. https://doi.org/10.3390/genes12121846
Ibero J, Galán B, García JL. Identification of the EdcR Estrogen-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor. Genes. 2021; 12(12):1846. https://doi.org/10.3390/genes12121846
Chicago/Turabian StyleIbero, Juan, Beatriz Galán, and José L. García. 2021. "Identification of the EdcR Estrogen-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor" Genes 12, no. 12: 1846. https://doi.org/10.3390/genes12121846