Starvation Protects Hepatocytes from Inflammatory Damage through Paradoxical mTORC1 Signaling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Antibodies
2.3. Cell Culture, Cell Stimulations and Treatments
2.4. Cell Death Assay and Quantification
2.5. Real-Time Glo Necrosis/Apoptosis Assay
2.6. siRNA Transfections
2.7. Western Blot and Immune Detection
2.8. Protein Synthesis Determination
2.9. Immunocytochemistry
2.10. Statistical Analysis
3. Results
3.1. Starvation Protects Immortalized Hepatocytes against Pro-Inflammatory Cytokine/PAMP-Induced Cell Damage
3.2. Nutrient- and Energy-Sensing Pathways Respond to Starvation in Hepatocytes
3.3. AMPK and mTORC1, but Not mTORC2, Mediate Starvation-Induced Protection against Inflammatory Stress
3.4. High Autophagy Rate Does Not Protect Hepatocytes from Inflammatory Damage
3.5. Protein Synthesis Rate Correlates with Cell Viability and Is Strongly Dependent on mTORC1
3.6. Starvation Induces Stress Granule Formation in Dependency on mTORC1, but Stress Granules Do Not Accumulate in Protected Cells
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Hussain, I.; Sureshkumar, H.K.; Bauer, M.; Rubio, I. Starvation Protects Hepatocytes from Inflammatory Damage through Paradoxical mTORC1 Signaling. Cells 2023, 12, 1668. https://doi.org/10.3390/cells12121668
Hussain I, Sureshkumar HK, Bauer M, Rubio I. Starvation Protects Hepatocytes from Inflammatory Damage through Paradoxical mTORC1 Signaling. Cells. 2023; 12(12):1668. https://doi.org/10.3390/cells12121668
Chicago/Turabian StyleHussain, Iqra, Harini K. Sureshkumar, Michael Bauer, and Ignacio Rubio. 2023. "Starvation Protects Hepatocytes from Inflammatory Damage through Paradoxical mTORC1 Signaling" Cells 12, no. 12: 1668. https://doi.org/10.3390/cells12121668