A Gluten-Free Diet during Pregnancy and Early Life Increases Short Chain Fatty Acid-Producing Bacteria and Regulatory T Cells in Prediabetic NOD Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Animals and Diets
2.3. Islet Autoantibodies
2.4. Histology
2.5. Quantitative PCR
2.6. Gut Microbiota
2.7. Statistical Analysis
3. Results
3.1. A Gluten-Free Diet Reduces Insulitis Degrees and Increases the Expression of FOXP3 and GATA3
3.2. Altered Beta Diversity of Gut Microbiota from NOD Mice Fed a Gluten-Free Diet
3.3. A GF Diet Changes the Gut Microbiome in NOD Mice
3.4. Differential Abundance of Butyrate-Producing and Lactobacillus Bacteria in GF and STD Mice Correlates with Insulitis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Family | Mean Abundance | Statistical Significance | ||
---|---|---|---|---|
GF | STD | p-Value | q-Value | |
Cecum 6 weeks | ||||
Lactobacillaceae | 5.1 | 270.9 | <0.0001 | 0.0001 |
Family_XIII | 18.6 | 6.8 | 0.0002 | 0.0014 |
Bacteroidales_S24-7_group | 301.7 | 1499.2 | 0.0002 | 0.0014 |
Lachnospiraceae | 4024.7 | 2832.9 | 0.0003 | 0.0014 |
Cecum 13 weeks | ||||
Lactobacillaceae | 8.2 | 452.3 | <0.0001 | <0.0001 |
Porphyromonadaceae | 330.0 | 93.6 | <0.0001 | <0.0001 |
Ruminococcaceae | 1320.3 | 778.8 | <0.0001 | <0.0001 |
Bacteroidales_S24-7_group | 851.6 | 1490.6 | 0.0002 | 0.0008 |
Desulfovibrionaceae | 148.0 | 36.9 | 0.0003 | 0.0011 |
Family_XIII | 16.6 | 6.4 | 0.0052 | 0.0156 |
Deferribacteraceae | 44.2 | 11.6 | 0.0098 | 0.0249 |
Christensenellaceae | 4.1 | 1.1 | 0.0251 | 0.0499 |
Colon 6 weeks of age | ||||
Christensenellaceae | 6.8 | 0.0 | 0.0007 | 0.0146 |
Clostridiales_vadinBB60_group | 122.0 | 39.9 | 0.0013 | 0.0146 |
Porphyromonadaceae | 649.7 | 218.2 | 0.0028 | 0.0204 |
Lactobacillaceae | 18.0 | 838.3 | 0.0053 | 0.0247 |
Family_XIII | 68.0 | 17.0 | 0.0056 | 0.0247 |
Alcaligenaceae | 21.9 | 4.0 | 0.0102 | 0.0375 |
Colon 13 weeks of age | ||||
Porphyromonadaceae | 490.1 | 112.5 | <0.0001 | 0.0007 |
Lactobacillaceae | 86.7 | 931.0 | 0.0002 | 0.0017 |
Ruminococcaceae | 942.6 | 533.0 | 0.0020 | 0.0132 |
Desulfovibrionaceae | 99.5 | 24.9 | 0.0024 | 0.0132 |
Family_XIII | 39.5 | 11.6 | 0.0073 | 0.0270 |
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Johansen, V.B.I.; Færø, D.; Buschard, K.; Kristiansen, K.; Pociot, F.; Kiilerich, P.; Josefsen, K.; Haupt-Jorgensen, M.; Antvorskov, J.C. A Gluten-Free Diet during Pregnancy and Early Life Increases Short Chain Fatty Acid-Producing Bacteria and Regulatory T Cells in Prediabetic NOD Mice. Cells 2023, 12, 1567. https://doi.org/10.3390/cells12121567
Johansen VBI, Færø D, Buschard K, Kristiansen K, Pociot F, Kiilerich P, Josefsen K, Haupt-Jorgensen M, Antvorskov JC. A Gluten-Free Diet during Pregnancy and Early Life Increases Short Chain Fatty Acid-Producing Bacteria and Regulatory T Cells in Prediabetic NOD Mice. Cells. 2023; 12(12):1567. https://doi.org/10.3390/cells12121567
Chicago/Turabian StyleJohansen, Valdemar Brimnes Ingemann, Daisy Færø, Karsten Buschard, Karsten Kristiansen, Flemming Pociot, Pia Kiilerich, Knud Josefsen, Martin Haupt-Jorgensen, and Julie Christine Antvorskov. 2023. "A Gluten-Free Diet during Pregnancy and Early Life Increases Short Chain Fatty Acid-Producing Bacteria and Regulatory T Cells in Prediabetic NOD Mice" Cells 12, no. 12: 1567. https://doi.org/10.3390/cells12121567