Sorghum as a Novel Crop for Central Europe: Using a Broad Diversity Set to Dissect Temperate-Adaptation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Germplasm
2.2. Phenotyping
2.3. Field Experiments for Chilling Stress and Juvenile Development
2.4. Field Experiments for Maturity and Bioenergy Related Agronomical Traits
2.5. Statistical Analysis of Phenotypic Data
2.6. Analysis of Genotypic Data
3. Results
3.1. Phenotypic Variation for Chilling Tolerance and Juvenile Development
3.2. Phenotypic Variation for Maturity and Bioenergy Related Agronomical Traits
3.3. Adaptation to Cooler Climates
3.4. Population Structure of the Diversity Panel
3.5. Genome Wide Association Study for Early Cold Tolerance Traits
3.6. Genome-Wide Association Studies for Agronomical Traits
4. Discussion
4.1. Phenotypic Variation for Chilling Tolerance and Juvenile Development
4.2. Phenotypic Variation for Maturity and Bioenergy Related Agronomical Traits
4.3. Lines with a Good Adaptation to Cooler Climates
4.4. Population Structure of the Diversity Panel
4.5. Genome-Wide Association Studies for Juvenile Chilling Tolerance
4.6. Genome-Wide Association Studies for Bioenergy Related Agronomical Traits
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CC | climate chamber |
DTF | days to flowering |
DMC | dry matter content |
EI | emergence index |
EM | emergence |
GWAS | genome wide association study |
LG | leaf greenness |
PH | plant height |
QTL | quantitative trait loci |
RCBD | randomized complete block design |
RDW | root dry weight |
RIL | recombinant inbred line |
SD | stem diameter |
SDW | shoot dry weight |
SNP | single nucleotide polymorphism |
References
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Site | Year | Trial Dates (Sowing-Harvest) | Mean Temp. (°C) | Mean Max. Temp. (°C) | Mean Min. Temp. (°C) | Absolute Max. and Min. Temp. (°C) | Mean Soil Temp. (°C) | Min. Soil Temp. (°C) | Precipitations (mm) |
---|---|---|---|---|---|---|---|---|---|
Giessen (GI) | 2013 | 5 June–5 July | 17.4 | 23.3 | 11.5 | 34.5/5.1 | 20.5 | 17.7 | 44 |
2014 | 6 May–5 June | 13.8 | 19.8 | 7.8 | 28.5/2.3 | 17.3 | 14.2 | 68 | |
Poel (PL) | 2013 | 5 June–8 July | 16.5 | 20.7 | 12.3 | 31.7/8.3 | 18.8 | 15.6 | 79 |
2014 | 6 May–10 June | 15.1 | 19.4 | 10.8 | 30.3/6.7 | 16.1 | 10.4 | 93 |
Site | Coordinates | Altitude | Soil Type | Day Length at Summer Solstice |
---|---|---|---|---|
Poel (PL) | 53°99′ N, 11°47′ E | 19 m | Loamy sand | 17 h 10 min |
Rauischholzhausen (RH) | 50°46′ N, 8°53′ E | 270 m | Loam | 16 h 31 min |
Giessen (GI) | 50°35′ N, 8°41′ E | 158 m | Clay | 16 h 29 min |
Gross-Gerau (GG) | 49°55′ N, 8°29′ E | 90 m | Sand | 16 h 22 min |
Site | Year | Traits Scored | Sowing Date | Climate Data from Sowing Until 10 October | ||
---|---|---|---|---|---|---|
Mean Max. Temperature (°C) | Mean Min. Temperature (°C) | Precipitations (mm) | ||||
Poel (PL) | 2013 | DTF, PH | 5 June | 21.0 | 12.5 | 195 |
2014 | DTF, PH | 6 May | 21.4 | 13.3 | 341 | |
Rauischholzhausen (RH) | 2013 | DTF, PH, DMC | 11 June | 22.2 | 9.1 | 240 |
2014 | DTF, PH, DMC | 21 May | 22.6 | 11.1 | 424 | |
Gross-Gerau (GG) | 2012 | DTF, PH, Brix, SD, DMC | 29 May | 24.3 | 11.6 | 338 |
2013 | DTF, PH, Brix, SD, DMC | 4 June | 24.4 | 12.1 | 275 | |
2014 | DTF, PH, Brix, SD, DMC | 20 May | 25.0 | 11.8 | 330 |
Experiment | df | Emergence (%) | Shoot Dry Weight (mg) (SDW) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
MS | Error | Mean | Min | Max | MS | Error | Mean | Min | Max | ||
GI 2013 | 289 | 382.3 *** | 191.2 | 56.3 | 22.5 | 87.5 | 15166.0 *** | 9097.4 | 215.1 | 43.4 | 1044.5 |
PL 2013 | 288 | 177.3 *** | 111.4 | 64.1 | 32.5 | 86.5 | 19299.4 *** | 9212.6 | 270.2 | 55 | 640 |
GI 2014 | 337 | 558.8 *** | 82.7 | 54.4 | 6 | 88 | 139.7 *** | 53.2 | 32.1 | 6.7 | 56.3 |
PL 2014 | 337 | 471.8 *** | 179.9 | 43.8 | 2.5 | 83 | 17210.5 *** | 10265.4 | 251.3 | 60 | 760 |
Trait | MS | Error | Mean | Min | Max |
---|---|---|---|---|---|
Emergence (%) | 1222.4 *** | 135.2 | 80.2 | 17.2 | 100 |
Emergence Index (EI) | 18.9 *** | 4.5 | 17.7 | 12.5 | 25.5 |
Shoot dry weight (SDW) (mg) | 7.9 *** | 0.9 | 5.6 | 2.1 | 9.2 |
Root dry weight (RDW) (mg) | 6.3 *** | 1.1 | 3.3 | 1.1 | 12.1 |
Leaf Greenness | 7.8 *** | 0.6 | 5.2 | 1 | 8.3 |
Source | df | Emergence | Shoot Dry Weight |
---|---|---|---|
Genotype (G) | 337 | 1209.2 *** | 16921.4 *** |
Environment (E) | 4 | 176414.4 *** | 12773831.5 *** |
G × E | 1243 | 342.5 *** | 9250.2 *** |
Error | 2245 | 138.2 | 3915.1 |
h2 | 0.74 | 0.41 |
Environment | Days to Flowering (DTF) | Dry Matter Content (%) (DMC) | Plant Height (cm) | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
df | MS | Error | Mean | Min | Max | df | MS | Error | Mean | Min | Max | df | MS | Error | Mean | Min | Max | |
GG | 329 | 211.7 *** | 119.6 | 77.1 | 51 | 111 | 334 | 30.8 *** | 6.4 | 29.2 | 18.9 | 45.6 | 329 | 3459.9 *** | 352.5 | 136 | 43 | 353 |
RH | 298 | 205.5 | 239.4 | 96 | 65 | 129 | 295 | 13.8 *** | 6.0 | 24.7 | 16.6 | 37.5 | 298 | 3895.7 *** | 505.6 | 136 | 42 | 330 |
PL | 317 | 192.2 *** | 72.3 | 100 | 67 | 130 | - | - | - | - | - | - | 329 | 4265.0*** | 507.8 | 142 | 60 | 330 |
Environment | Brix (%) | Stem Diameter (cm) (SD) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
df | MS | Error | Mean | Min | Max | df | MS | Error | Mean | Min | Max | |
GG 2012 | 321 | 52.4 *** | 4.0 | 12.4 | 3.3 | 21.3 | 321 | 0.42 *** | 0.04 | 1.75 | 0.7 | 2.77 |
GG 2013 | 333 | 41.7 *** | 3.3 | 13.5 | 3.3 | 21 | 374 | 0.42 *** | 0.04 | 1.63 | 0.77 | 2.7 |
GG 2014 | 337 | 45.8 *** | 3.2 | 13.7 | 4 | 22.3 | 378 | 0.39 *** | 0.03 | 1.63 | 0.63 | 3.13 |
Source | DTF | Dry Matter Content | Plant Height | Brix | Stem Diameter | |||||
---|---|---|---|---|---|---|---|---|---|---|
df | ms | df | ms | df | ms | df | ms | df | ms | |
Genotype (G) | 334 | 397.9 *** | 337 | 37.3 *** | 334 | 9307.7 *** | 337 | 91.4 *** | 337 | 0.93 *** |
Environment (E) | 2 | 110119.4 *** | 1 | 7489.0 *** | 2 | 22511.8 *** | 2 | 412.1 *** | 2 | 5.47 *** |
G × E | 610 | 89.0 | 337 | 7.0 | 610 | 533.4 ** | 654 | 23.4 *** | 654 | 0.14 *** |
Error | 614 | 141.4 | 863 | 6.4 | 614 | 454.5 | 1987 | 3.5 | 1987 | 0.04 |
h² | 0.80 | 0.83 | 0.96 | 0.72 | 0.82 |
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Schaffasz, A.; Windpassinger, S.; Friedt, W.; Snowdon, R.; Wittkop, B. Sorghum as a Novel Crop for Central Europe: Using a Broad Diversity Set to Dissect Temperate-Adaptation. Agronomy 2019, 9, 535. https://doi.org/10.3390/agronomy9090535
Schaffasz A, Windpassinger S, Friedt W, Snowdon R, Wittkop B. Sorghum as a Novel Crop for Central Europe: Using a Broad Diversity Set to Dissect Temperate-Adaptation. Agronomy. 2019; 9(9):535. https://doi.org/10.3390/agronomy9090535
Chicago/Turabian StyleSchaffasz, André, Steffen Windpassinger, Wolfgang Friedt, Rod Snowdon, and Benjamin Wittkop. 2019. "Sorghum as a Novel Crop for Central Europe: Using a Broad Diversity Set to Dissect Temperate-Adaptation" Agronomy 9, no. 9: 535. https://doi.org/10.3390/agronomy9090535