Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production of Cochleates from S. Infantis
2.1.1. Isolation of S. Infantis
2.1.2. Bacterial Membrane Purification
2.1.3. Cochleate Formation
2.1.4. Cochleate Characterization
2.2. Cochleate Encapsulation
2.3. Characterization of Encapsulated Cochleates
2.3.1. Appearance and Color
2.3.2. Size
2.3.3. Morphology
2.3.4. Protein Content
2.3.5. Fourier Transform Infrared Spectroscopy (FTIR)
2.4. In Vivo Safety Trial
2.5. Statistical Analysis
3. Results
3.1. Cochleate Development
3.2. Cochleate Encapsulation and Characterization
3.3. In Vivo Safety Trial
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Parameters | Signs | Score |
---|---|---|
Weight loss | No alterations Weight loss below 10% Weight loss between 10–20% Weight loss greater than 20% | 0 1 2 3 |
Aspect | No alterations Ruffled feathers Ruffled feathers + wings and tail dropped Ruffled feathers + wings and tail dropped + dirty tail | 0 1 2 3 |
Behavior | No alterations Feeding activity decreased (observed at feeding time) Careless of environment, sagging through their legs to sitting position Depressed birds—birds with stupor | 0 1 2 3 |
Vital signs | No alterations Increment until 2 °C in temperature Increment greater than 2 °C in temperature Previous signs + change in cardiac and respiratory frequency | 0 1 2 3 |
Parameters | Microparticles | |||
MP-0% | MP-5% | MP-10% | MP-15% | |
Color | ||||
L* | 91.5 ± 1.2a | 90.3 ± 0.9a | 89.1 ± 1.1a | 89.6 ± 1.3a |
a* | -1.1 ± 0.2a | -1.2 ± 0.4a | -0.9 ± 0.2a | -1.2 ± 0.4a |
b* | 1.7 ± 0.3a | 1.8 ± 0.4a | 1.5 ± 0.2a | 1.6 ± 0.4a |
Size (µm) | 10.4 ± 0.5 | 15.8 ± 0.9 | 16.9 ± 0.5 | 10.6 ± 0.1 |
Protein content (mg/g) | n.d | 0.7 ± 0.3a | 1.4 ± 0.2b | 1.8 ± 0.3c |
Beads | ||||
B-0% | B-5% | B-10% | B-15% | |
Color | ||||
L* | 33.4 ± 1.5a | 28.1 ± 1.8b | 27.8 ± 1.3b | 29.2 ± 1.1b |
a* | 1.3 ± 0.5a | 2.5 ± 0.3b | 2.4 ± 0.7b | 2.6 ± 0.3b |
b* | 1.7 ± 0.4a | 4.6 ± 0.4b | 4.7 ± 0.8b | 4.8 ± 0.6b |
Size (µm) | 1,680 ± 82a | 1,620 ± 36a | 1,700 ± 18a | 1,950 ± 28b |
Protein content (mg/g) | n.d | 1.0 ± 0.2a | 1.7 ± 0.4b | 2.5 ± 0.3c |
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Avendaño, C.; Vidal, S.; Villamizar-Sarmiento, M.G.; Guzmán, M.; Hidalgo, H.; Lapierre, L.; Valenzuela, C.; Sáenz, L. Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine. Polymers 2021, 13, 3426. https://doi.org/10.3390/polym13193426
Avendaño C, Vidal S, Villamizar-Sarmiento MG, Guzmán M, Hidalgo H, Lapierre L, Valenzuela C, Sáenz L. Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine. Polymers. 2021; 13(19):3426. https://doi.org/10.3390/polym13193426
Chicago/Turabian StyleAvendaño, Constanza, Sonia Vidal, María Gabriela Villamizar-Sarmiento, Miguel Guzmán, Héctor Hidalgo, Lisette Lapierre, Carolina Valenzuela, and Leonardo Sáenz. 2021. "Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine" Polymers 13, no. 19: 3426. https://doi.org/10.3390/polym13193426