Differentiating Co-Delivery of Bisphosphonate and Simvastatin by Self-Healing Hyaluronan Hydrogel Formed by Orthogonal “Clicks”: An In-Vitro Assessment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of 2-(2-Pyridyldithio)-2-Methylethanol 2
2.2. Synthesis of 2-(2-Pyridyldithio)-2-Methylethyl N-Hydroxysuccinimide Carbonate 3
2.3. Synthesis of Releasable 2-Pyridyldithio Derivative of Pamidronate 4
2.4. Synthesis of 2-Pyridyldithio Derivative of N-(6-Aminohexyl)-2,4-Dinitroaniline 5
2.5. Synthesis of Aldehyde-Modified DN Derivative 6
2.6. Synthesis of Releasable HA-//-BP Conjugate
2.7. Synthesis of Releasable BP-HA-//-DN Conjugate and Its Characterization by Dynamic Light Scattering (DLS)
2.8. Synthesis of hy-HA-//-BP Derivative
2.9. Synthesis of DN-hyd-HA-//-BP Derivative and Simvastatin Loading
2.10. Formation of HA-//-BP•Ca2+ Hydrogel and Its Thiol-Triggered Dissolution
2.11. Formation of DN-//-HA-BP•Ca2+ Hydrogel
2.12. Study of DN Release from DN-//-HA-BP•Ca2+ Hydrogel
2.13. Study of Simvastatin Release from SIM@DN-//-HA-BP•Ca2+ Nanogel
2.14. Statistical Analysis
3. Results and Discussion
3.1. Synthesis of Releasable HA-//-BP Conjugate
3.2. Coordination Hydrogel Formed by Releasable HA-//-BP
3.3. Synthesis and Characterization of Releasable BP-HA-//-DN Conjugate
3.4. Thiol-Triggered Release of Covalently Linked Drugs
3.5. Synthesis of DN-hyd-HA-//-BP Conjugate with Dual Release Mechanism
3.6. Acid-Triggered Release of DN Imaging Groups and Physically Encapsulated Simvastatin
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ossipov, D.A.; Lüchow, M.; Malkoch, M. Differentiating Co-Delivery of Bisphosphonate and Simvastatin by Self-Healing Hyaluronan Hydrogel Formed by Orthogonal “Clicks”: An In-Vitro Assessment. Polymers 2021, 13, 2106. https://doi.org/10.3390/polym13132106
Ossipov DA, Lüchow M, Malkoch M. Differentiating Co-Delivery of Bisphosphonate and Simvastatin by Self-Healing Hyaluronan Hydrogel Formed by Orthogonal “Clicks”: An In-Vitro Assessment. Polymers. 2021; 13(13):2106. https://doi.org/10.3390/polym13132106
Chicago/Turabian StyleOssipov, Dmitri A., Mads Lüchow, and Michael Malkoch. 2021. "Differentiating Co-Delivery of Bisphosphonate and Simvastatin by Self-Healing Hyaluronan Hydrogel Formed by Orthogonal “Clicks”: An In-Vitro Assessment" Polymers 13, no. 13: 2106. https://doi.org/10.3390/polym13132106