Bio-Catalytic Activity of Novel Mentha arvensis Intervened Biocompatible Magnesium Oxide Nanomaterials
, Hasnain Jan 1,3, Sajjad Ali Shah 1, Sumaira Shah 4, Muhammad Rizwan 5, Nasib Zaman 5, Zahid Hussain 5, Muhammad Nazir Uddin 5, Nadia Bibi 6, Aishma Khattak 7, Wajid Khan 5, Arshad Iqbal 5, Muhammad Idrees 8 and Rehana Masood 9Round 1
Reviewer 1 Report
Please see attached file
Comments for author File: 
Comments.pdf
Author Response
Point 1:
Introduction
“The physico-morphic properties of MgO nanoparticles depend-ing on the reaction time and condition [13-15]” – Please specify the properties, e.g., size, shape, crystallinity.
Response 1: comments were addressed and highlighted as Yellow
Please mention the relation of specific properties on the reaction time and conditions of synthesis.
Response 1: comments were addressed and highlighted as Yellow
Point 2
Have the authors taken a look at the following article: El-Moslamy et al.
Response 1: yes we look the following article: El-Moslamy et al.
Point 3
“Bioprocessing strategies for cost-effective large-scale biogenic synthesis of nano-MgO from endophytic Streptomyces coelicolorstrain E72 as an anti-multidrug-resistant pathogens agent”, Scientific Reports, 2018, 8:3820, DOI: 10.1038/s41598-018-22134? Please cite in the introduction.
Response 1: The above-mentioned articles has been cited in introduction section of our article
Point 4
Results
Please explain the A, B, and C subsections of Figure 1 in the figure caption.
Response 1: we explain and correct.
Point 5
Are there specific phytochemicals that are forming the ligand coating on the MgO NPs?
Response 1: yes
Point 6
Figure 2a: The symbol μ in the x and y-axes titles should be replaced by ν representing frequency of light. The figure should show the line extrapolating the linear region of the TauC plot to determine the band gap. The band gap appears to be around 3.3 eV from the plot.“In both cases, the bang distance of MgO-NPs was estimated to be (3.4eV)” – The TauC plot was used to find the direct band gap for the NPs. The sentence should be modified to reflect this.
Response 2: Mistakes has been removed
Point 6
“As a result of the inclusion of more plant materials in bio, we found that the highest depletion of MgO-NPs occurs.” – Please explain in more details.
Response 2: we explain and the comments were addressed and highlighted as Yellow
Point 7
Figure 2d shows four stages of weight loss. Please explain if these are due to breaking of any specific chemical bonds of the precursor. A control TGA of just the precursor should be ran to understand the formation mechanism of the MgO NPs.
Response 2: we explain and the comments is cleared and highlighted as yellow in the manuscript.
Point 8
“SEM was used to investigate the scale, distribution, and morphology of MgO-NPs, as seen in Figure 3(a).” – Please use size instead of scale.
Response 2: (Corrected)
Point 9
“Figure 3(b) shows TEM micrographs of syn-thesised NPs with circular or elliptical morphology and a mean size of 29.7211.36nm.” – What do the authors mean by this size: 29.7211.36 nm? Please correct the size.
Response 2: (Corrected)
Point 10
Figure 3c- Please show the x-axis up to 10 keV as there are no peaks after this range.
Response 2: The comment has been addressed.
Point 11
“The DLS technique is used to investigate the size distribution and zeta potential (z) of biosynthesized MgO-NPs” – Please use the symbol ζ for zeta potential.
Response 2: comments were addressed and highlighted as Yellow
Point 12
“Biomolecules in plant extract and there possible re-duction in nanoparticles leads to negative surface charge” – The author means capping or coating of the biomolecules on the surface of nanoparticles instead of reduction.
Response 2: Corrected and followed in manuscript)
Point 13
“The Zeta hydrodynamic size were 30 to 120nm observed via DLS as shown in Figure 4” – Please mention the polydispersity index of the sample. Please also mention the standard deviation for the
zeta potential.
Response 2: Corrected and followed in manuscript)
Point 14
“These metabolites processed on the surface of the body have a similar relationship [36]” – Please explain this in a few more details.
Response 2: Corrected and followed in manuscript)
Author Response File: Author Response.pdf
Reviewer 2 Report
This study focuses on an environmental friendly synthesis of MgO-NPs from Mentha arvensis. The authors concluded that the biogenic MgO-NPs described above can be used in a variety of diseases, cosmetics, and cancer studies. More research into the use of magnesium oxide nanoparticles in biomedicine, both in vitro and in vivo, is required.
Most of the manuscript is discribition. The mechanism of synthesis and bioactivities is very poor, the authors shoud concern more of the mechanism.
Author Response
Point 1: This study focuses on an environmentally friendly synthesis of MgO-NPs from Mentha arvensis. The authors concluded that the biogenic MgO-NPs described above can be used in a variety of diseases, cosmetics, and cancer studies. More research into the use of magnesium oxide nanoparticles in biomedicine, both in vitro and in vivo, is required.
Response 1: We tried our best to address this comment of our respected reviewer but due to COVID-19 pandemic, institutes and research centers are not functional .
Point 2: Most of the manuscript is discribition. The mechanism of synthesis and bioactivities is very poor, the authors shoud concern more of the mechanism.
Response 2: : We have tried our best and improved.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Accept
