CD14 Expressing Precursors Give Rise to Highly Functional Conventional Dendritic Cells for Use as Dendritic Cell Vaccine
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. CB Collection and CD34 Isolation
2.2. CB-DC Culture
2.3. DC Phenotype
2.4. Uptake Assay
2.5. Mixed Leukocyte Reaction (MLR)
2.6. WT1 Antigen Presentation
2.7. RNA-Sequence
2.8. Statistical Analysis
3. Results
3.1. Heterogenic CD14 Expression within CB-Derived CD115+ DC Precursors
3.2. CD14 Discriminates Two Distinct Differentiated CD1c Populations
3.3. CD123+DC and CD1c++DC Exhibit Functional Differences
3.4. CD123+DCs and CD1c++DCs Have a Distinct T-Cell Stimulation Capacity
3.5. Translation towards a Clinical Grade Protocol
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Plantinga, M.; van den Beemt, D.A.M.H.; Dünnebach, E.; Nierkens, S. CD14 Expressing Precursors Give Rise to Highly Functional Conventional Dendritic Cells for Use as Dendritic Cell Vaccine. Cancers 2021, 13, 3818. https://doi.org/10.3390/cancers13153818
Plantinga M, van den Beemt DAMH, Dünnebach E, Nierkens S. CD14 Expressing Precursors Give Rise to Highly Functional Conventional Dendritic Cells for Use as Dendritic Cell Vaccine. Cancers. 2021; 13(15):3818. https://doi.org/10.3390/cancers13153818
Chicago/Turabian StylePlantinga, Maud, Denise A. M. H. van den Beemt, Ester Dünnebach, and Stefan Nierkens. 2021. "CD14 Expressing Precursors Give Rise to Highly Functional Conventional Dendritic Cells for Use as Dendritic Cell Vaccine" Cancers 13, no. 15: 3818. https://doi.org/10.3390/cancers13153818