A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Apparatus
2.2. Optimization of Detection Conditions
2.3. Colorimetric Detection of ATX-a
3. Results and Discussion
3.1. Feasibility of the Colorimetric Assay for ATX-a Detection
3.2. Optimization of the Detection Conditions
3.3. Sensitivity, Specificity, Repeatability, and Stability of the Colorimetric Assay for ATX-a Detection
3.4. Analysis of Real Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Methods | Linear Ranges | Limit of Detection | Reference |
---|---|---|---|
Electrochemistry | 1–100 nM | 0.5 nM | [1] |
HPLC a | 0.3–9.078 μM | 121 nM | [4] |
GC–MS b | 2.5–200 ng/mL | 2 ng/mL | [5] |
Fluorescence | – | <3 nM | [6] |
Electrochemiluminescence | 1–1000 mg/L | 0.34 mg/L | [7] |
Colorimetric aptasensor | 10 pM–200 nM | 4.45 pM | Present study |
Samples | Added ATX-a (nM) | Found ATX-a (nM) | Recovery (%) | RSD (%) |
---|---|---|---|---|
Lake water 1 | 0.1 | 0.08972 | 89.72 | 8.15 |
Lake water 2 | 0.5 | 0.4519 | 90.38 | 10.91 |
Lake water 3 | 1 | 1.0419 | 104.19 | 2.98 |
Lake water 4 | 50 | 56.215 | 112.43 | 6.37 |
Lake water 5 | 200 | 195.7 | 97.85 | 4.12 |
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Nguyen, D.-K.; Jang, C.-H. A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles. Micromachines 2021, 12, 1526. https://doi.org/10.3390/mi12121526
Nguyen D-K, Jang C-H. A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles. Micromachines. 2021; 12(12):1526. https://doi.org/10.3390/mi12121526
Chicago/Turabian StyleNguyen, Duy-Khiem, and Chang-Hyun Jang. 2021. "A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles" Micromachines 12, no. 12: 1526. https://doi.org/10.3390/mi12121526