Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest Paralipsa gularis (Zeller)
Abstract
:1. Introduction
2. Results
2.1. Susceptibility of P. gularis to Bt Insecticidal Proteins
2.2. Insecticidal Protein Expression Content and Insecticidal Activity of Different Tissues in Bt-(Cry1Ab+Vip3Aa) Maize
2.3. Control Efficiency of Bt-(Cry1Ab+Vip3Aa) Maize against P. gularis
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Collection and Rearing of P. gularis
5.2. Determination of the Susceptibility of P. gularis to Bt Insecticidal Proteins
5.3. Determination of Insecticidal Protein Expression Content and Insecticidal Activity in Different Tissues of Bt-(Cry1Ab+Vip3Aa) Maize
5.4. Field Surveys of the Control Efficiency of Bt-(Cry1Ab+Vip3Aa) Maize against P. gularis
5.5. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bt Event | Protein | N | LC50 (95%FL) μg/g | LC95 (95%FL) μg/g | Slope ± SE | χ2 | df |
---|---|---|---|---|---|---|---|
DBN9936 | DBNCry1Ab | 432 | 0.038 (0.013–0.062) b | 0.276 (0.207–0.455) c | 1.916 ± 0.406 | 13.043 | 13 |
DBN9501 | DBNVip3Aa | 432 | 0.114 (0.088–0.150) a | 2.513 (1.225–8.564) a | 1.224 ± 0.169 | 18.141 | 13 |
DBN3601T | DBN Cry1Ab+Vip3Aa | 432 | 0.110 (0.081–0.136) a | 0.606 (0.463–0.917) b | 2.217 ± 0.288 | 16.306 | 13 |
Bt11×MIR162 | Syngenta Cry1Ab+Vip3Aa | 432 | 0.147 (0.107–0.184) a | 0.866 (0.657–1.321) ab | 2.138 ± 0.277 | 5.096 | 13 |
Bt Event | Protein | N | GIC50 (95%FL) μg/g | GIC95 (95%FL) μg/g | Slope ± SE | χ2 | df |
---|---|---|---|---|---|---|---|
DBN9936 | DBNCry1Ab | 432 | 0.014 (0.000–0.035) b | 0.072 (0.020–0.096) c | 2.343 ± 0.861 | 4.965 | 13 |
DBN9501 | DBNVip3Aa | 432 | 0.073 (0.061–0.085) a | 0.249 (0.199–0.349) a | 3.088 ± 0.176 | 34.555 | 10 |
DBN3601T | DBN Cry1Ab+Vip3Aa | 432 | 0.027 (0.010–0.043) b | 0.129 (0.108–0.154) b | 2.442 ± 0.482 | 3.511 | 13 |
Bt11×MIR162 | Syngenta Cry1Ab+Vip3Aa | 432 | 0.026 (0.006–0.048) b | 0.160 (0.122–0.196) b | 2.068 ± 0.450 | 1.501 | 13 |
Growth Stage | Tissue | Cry1Ab (μg/g) | Vip3Aa (μg/g) | Total Bt Protein (μg/g) | |||
---|---|---|---|---|---|---|---|
Baozang Town | Longtan Town | Baozang Town | Longtan Town | Baozang Town | Longtan Town | ||
R3 | Kernel | - | 1.02 ± 0.04 | - | 1.53 ± 0.04 | - | 2.55 ± 0.01 |
Cob | - | 6.48 ± 1.20 | - | 0.56 ± 0.05 | - | 7.04 ± 1.25 | |
Stalk | - | 21.60 ± 0.55 | - | 0.64 ± 0.02 | - | 22.24 ± 0.56 | |
Bract | - | 7.62 ± 0.37 | - | 0.27 ± 0.03 | - | 7.89 ± 0.35 | |
R5 | Kernel | 1.66 ± 0.30 | 2.42 ± 0.10 | 0.80 ± 0.04 | 2.19 ± 0.05 | 2.46 ± 0.29 | 4.61 ± 0.15 |
Cob | 7.40 ± 0.68 | 4.89 ± 0.40 | 0.18 ± 0.03 | 0.06 ± 0.02 | 7.58 ± 0.68 | 4.95 ± 0.38 | |
Stalk | 14.19 ± 2.96 | 15.46 ± 0.92 | 0.42 ± 0.03 | 0.74 ± 0.02 | 14.60 ± 2.97 | 16.20 ± 0.91 | |
Bract | 15.24 ± 0.31 | 2.65 ± 0.18 | 0.20 ± 0.02 | 0.07 ± 0.00 | 15.44 ± 0.33 | 2.72 ± 0.18 | |
R6 | Kernel | 1.12 ± 0.08 | 1.04 ± 0.02 | 0.20 ± 0.02 | 0.80 ± 0.03 | 1.40 ± 0.07 | 1.84 ± 0.02 |
Cob | 3.63 ± 0.32 | 3.79 ± 0.11 | 0.01 ± 0.00 | 0.15 ± 0.02 | 3.64 ± 0.32 | 3.94 ± 0.08 | |
Stalk | 14.09 ± 1.22 | 14.99 ± 0.49 | 0.12 ± 0.01 | 0.29 ± 0.02 | 14.22 ± 1.23 | 15.29 ± 0.48 | |
Bract | 7.62 ± 3.50 | 8.32 ± 0.87 | 0.02 ± 0.01 | 0.28 ± 0.01 | 7.64 ± 3.50 | 8.59 ± 0.87 |
Tissue | Maize Variety | R3 | R5 | R6 | |||
---|---|---|---|---|---|---|---|
Baozang Town | Longtan Town | Baozang Town | Longtan Town | Baozang Town | Longtan Town | ||
Kernel | Bt-(Cry1Ab+Vip3Aa) maize | 100.00 ± 0.00 * | 99.00 ± 1.00 * | 99.50 ± 0.50 * | 99.00 ± 0.58 * | 100.00 ± 0.00 * | 99.00 ± 0.58 * |
Conventional maize | 17.50 ± 1.26 | 20.50 ± 1.71 | 13.00 ± 1.29 | 18.50 ± 2.36 | 55.00 ± 3.00 | 27.50 ± 1.71 | |
Cob | Bt-(Cry1Ab+Vip3Aa) maize | 98.50 ± 0.96 * | 98.00 ± 1.15 * | 100.00 ± 0.00 * | 100.00 ± 0.00 * | 99.00 ± 0.58 * | 99.50 ± 0.50 * |
Conventional maize | 55.50 ± 3.69 | 19.50 ± 4.50 | 31.50 ± 2.63 | 63.50 ± 3.20 | 44.00 ± 3.92 | 29.50 ± 2.99 | |
Stalk | Bt-(Cry1Ab+Vip3Aa) maize | 99.00 ± 0.58 * | 100.00 ± 0.00 * | 99.50 ± 0.50 * | 100.00 ± 0.00 * | 98.00 ± 0.82 * | 99.50 ± 0.50 * |
Conventional maize | 47.00 ± 1.92 | 37.00 ± 5.80 | 41.50 ± 4.79 | 51.00 ± 4.12 | 42.50 ± 6.60 | 24.00 ± 4.83 | |
Bract | Bt-(Cry1Ab+Vip3Aa) maize | 93.50 ± 2.22 * | 98.50 ± 0.96 * | 100.00 ± 0.00 * | 97.00 ± 0.58 * | 100.00 ± 0.00 * | 100.00 ± 0.00 * |
Conventional maize | 76.00 ± 2.31 | 75.50 ± 1.89 | 85.00 ± 4.44 | 66.50 ± 7.63 | 87.50 ± 2.50 | 74.50 ± 3.30 |
Survey Year | Maize Variety | R3 | R5 | R6 | |||
---|---|---|---|---|---|---|---|
Numbers of Larvae per 100 Plants | Plant Damage Rates (%) | Numbers of Larvae per 100 Plants | Plant Damage Rates (%) | Numbers of Larvae per 100 Plants | Plant Damage Rates (%) | ||
2022 year | Bt-(Cry1Ab+Vip3Aa) maize | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
Conventional maize | 208.90 ± 27.53 * | 60.30 ± 5.18 * | 104.80 ± 13.06 * | 75.80 ± 5.60 * | 422.10 ± 44.70 * | 94.40 ± 3.22 * | |
2023 year | Bt-(Cry1Ab+Vip3Aa) maize | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
Conventional maize | 6.00 ± 2.15 * | 3.40 ± 0.73 * | 0.80 ± 0.61 | 0.4 ± 0.27 | 1.40 ± 0.99 | 0.80 ± 0.44 |
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Chen, S.; Wang, W.; Kang, G.; Yang, X.; Wu, K. Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest Paralipsa gularis (Zeller). Toxins 2024, 16, 92. https://doi.org/10.3390/toxins16020092
Chen S, Wang W, Kang G, Yang X, Wu K. Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest Paralipsa gularis (Zeller). Toxins. 2024; 16(2):92. https://doi.org/10.3390/toxins16020092
Chicago/Turabian StyleChen, Shuang, Wenhui Wang, Guodong Kang, Xianming Yang, and Kongming Wu. 2024. "Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest Paralipsa gularis (Zeller)" Toxins 16, no. 2: 92. https://doi.org/10.3390/toxins16020092