Neutralization Capacity of Tissue Alterations Caused by the Venoms of the Most Dangerous Scorpions in North Africa Using a Selective Antivenom
Abstract
:1. Introduction
2. Results
2.1. Median Lethal Dose (LD50) and Sublethal Doses (sLD) of the Scorpions’ Venoms
2.2. The Neutralization Capacity of the Scorpion Antivenom (ED50)
2.3. Clinical Symptoms Observed by the Effect of Scorpion Venoms with or without Antivenom
2.4. Efficacity of Antivenom at Neutralizing the Physiopathological Effects of Scorpion Venoms
2.4.1. The Tissular Alterations Observed 4 h Following the Experimental Scorpionic Envenomation
2.4.2. Neutralizing Ability of the Antivenom Injected in Complexation with the Scorpion Venoms
2.4.3. Effectiveness of the Antivenom Injected after 2 h Following Scorpion Venom Inoculation
2.5. Tissue Localization of the Venom and the Complex Venom–Antivenom
2.5.1. Immunohistochemical Staining of the Am Venom and the Complex Venom–Antivenom in the Brain and Heart
2.5.2. Immunohistochemical Staining of Aah Venom and Venom and the Complex Venom–Antivenom in the Brain and Heart
2.5.3. Immunohistochemical Staining of Bo Venom and Venom and the Complex Venom–Antivenom in the Brain and Heart
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Venom and Antivenom
5.2. Mice
5.3. Median Lethal Dose (LD50) and Sublethal Doses (sLD) of the Scorpion Venoms
5.4. Median Effective Dose 50% (ED50) of the Scorpion Antivenom
5.5. Experimental Design
5.6. Histopathological Studies
5.7. Immunohistochemistry
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Am Venom | Aah Venom | Bo Venom | |
---|---|---|---|
LD50 (µg/kg) | 302 ± 60 | 597 ± 41 | 890.1 ± 10.7 |
sLD (µg/kg) | 135 ± 1.9 | 255 ± 6.1 | 415 ± 87 |
ED50 | Am Venom | Aah Venom | Bo Venom |
---|---|---|---|
in (µL) * | 59.14 ± 1.28 | 57.57 ± 0.29 | 38.73 ± 0.55 |
in (number of LD50 per mL of antivenom) ** | 50.7 | 52.1 | 77.4 |
Paralysis of the Lower Limbs | Chills of the Lower Limbs | Hunchback | Agitation | ||
---|---|---|---|---|---|
Am | Am Venom | +++ | ++ | ++ | +++ |
Complex Venom + Antivenom | - | - | - | - | |
Venom + Antivenom (2 h delay) | - | - | + | + | |
Aah | Aah Venom | ++ | + | +++ | +++ |
Complex Venom + Antivenom | - | - | - | - | |
Venom + Antivenom (2 h delay) | - | - | - | + | |
Bo | Bo Venom | ++ | + | ++ | +++ |
Complex Venom + Antivenom | - | - | - | - | |
Venom + Antivenom (2 h delay) | - | - | - | + |
Am Scorpion Venom | Aah Scorpion Venom | Bo Scorpion Venom | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Tissular alterations | Am Venom | Complex V + AV | V+ AV (2 h Delay) | Aah Venom | Complex V + AV | V+ AV (2 h Delay) | Bo Venom | Complex V + AV | V+ AV (2 h Delay) | |
Brain | Loss of cellularity | x | - | - | x | - | - | x | - | - |
Vasodilatation | x | - | x | x | - | - | x | - | x | |
Edema | x | x | - | x | - | x | - | - | - | |
Hemosiderin deposits | x | - | - | - | - | - | - | - | - | |
Hemorrhage | - | - | - | - | - | - | x | - | - | |
Heart | Degeneration of myofibers | x | - | - | x | - | x | x | - | x |
Congestion | x | - | x | x | - | - | x | - | x | |
Infiltration of inflammatory cells | - | - | - | x | - | - | - | - | - | |
Lungs | Alveolar destruction | x | - | - | x | - | - | x | - | - |
Alveolar space enlargement | x | - | - | x | - | - | x | - | - | |
Infiltration of inflammatory cells | x | - | - | - | - | - | x | - | x | |
Congestion | x | x | x | x | - | x | x | - | x | |
Pulmonary emphysema | x | - | - | - | - | - | - | - | - | |
Pulmonary thrombi | x | - | - | x | - | - | - | - | - | |
Liver | Centro-lobular congestion | x | - | - | x | - | x | x | - | - |
Sinusoidal dilation | x | - | - | x | - | - | - | - | - | |
Sinusoidal congestion | x | - | x | - | - | - | - | - | - | |
Infiltration of inflammatory cells | x | - | - | - | - | - | x | - | - | |
Necrosis | x | - | - | x | - | - | x | - | - | |
Hemorrhage | x | - | - | - | - | - | - | - | - | |
Kidneys | Enlargement of tubular lumen | x | - | x | x | - | x | x | - | - |
Glomerular disorganization | x | - | x | x | - | x | x | - | x | |
Congestion | x | - | - | x | - | - | x | - | - | |
Hemorrhage | - | - | x | x | - | - | - | - | - |
Group | Type of Staining | Type of Cell | Percentage | |
---|---|---|---|---|
Brain | Am Venom | Cytoplasmic Membrane |
| 40 |
Complex Venom + Antivenom | Cytoplasmic Membrane |
| 10 | |
Venom + Antivenom (2 h delay) | Cytoplasmic Membrane |
| 30 | |
Heart | Am Venom | Cytoplasmic Membrane |
| 60 |
Complex Venom + Antivenom | Cytoplasmic Membrane |
| 20 | |
Venom + Antivenom (2 h delay) | Cytoplasmic Membrane |
| 40 |
Group | Type of Staining | Type of Cell | Percentage | |
---|---|---|---|---|
Brain | Aah Venom | Membrane |
| 50 |
Complex Venom + Antivenom | Membrane |
| Less than 5 | |
Venom + Antivenom (2 h delay) | Membrane |
| 30 | |
Heart | Aah Venom | Cytoplasmic Membrane |
| 80 |
Complex Venom + Antivenom | Cytoplasmic Membrane |
| 30 | |
Venom + Antivenom (2 h delay) | Cytoplasmic Membrane |
| 50 |
Group | Type of Staining | Type of Cell | Percentage | |
---|---|---|---|---|
Brain | Bo Venom | Cytoplasmic Membrane |
| 40 |
Complex Venom + Antivenom | Cytoplasmic Membrane |
| 10 | |
Venom + Antivenom (2 h delay) | Cytoplasmic Membrane |
| 20 | |
Heart | Bo Venom | Cytoplasmic Membrane |
| 60 |
Complex Venom + Antivenom | Cytoplasmic Membrane |
| 30 | |
Venom + Antivenom (2 h delay) | Cytoplasmic Membrane |
| 50 |
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Darkaoui, B.; Aksim, M.; Aarab, A.; Lafnoune, A.; Khourcha, S.; Cadi, R.; Filali, O.A.; Oukkache, N. Neutralization Capacity of Tissue Alterations Caused by the Venoms of the Most Dangerous Scorpions in North Africa Using a Selective Antivenom. Toxins 2024, 16, 16. https://doi.org/10.3390/toxins16010016
Darkaoui B, Aksim M, Aarab A, Lafnoune A, Khourcha S, Cadi R, Filali OA, Oukkache N. Neutralization Capacity of Tissue Alterations Caused by the Venoms of the Most Dangerous Scorpions in North Africa Using a Selective Antivenom. Toxins. 2024; 16(1):16. https://doi.org/10.3390/toxins16010016
Chicago/Turabian StyleDarkaoui, Bouchra, Mohamed Aksim, Ayoub Aarab, Ayoub Lafnoune, Soukaina Khourcha, Rachida Cadi, Ouafaa Aniq Filali, and Naoual Oukkache. 2024. "Neutralization Capacity of Tissue Alterations Caused by the Venoms of the Most Dangerous Scorpions in North Africa Using a Selective Antivenom" Toxins 16, no. 1: 16. https://doi.org/10.3390/toxins16010016