Alleviation of Irritable Bowel Syndrome-Like Symptoms and Control of Gut and Brain Responses with Oral Administration of Dolichos lablab L. in a Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of D. lablab L.
2.2. Standardization of DL
2.3. Animal Experiments
2.4. Macroscopic Scoring of Zymosan-Induced Colon Changes
2.5. Body Weight Changes and Food Intake
2.6. Histological Examination
2.7. Polymerase Chain Reaction (PCR)
2.8. Murine Bone Marrow-Derived Mast Cells Culture
2.9. Enzyme-Linked Immunosorbent Assay (ELISA)
2.10. Pain-Related Behavior Test
2.11. Anxiety-Related Behavior Test
2.12. Immunofluorescence Analysis (IF)
2.13. Western Blotting
2.14. Statistical Analysis
3. Results
3.1. Standardization of DL
3.2. Effects of DL on the Macroscopic Score of Zymosan-Induced Colon Changes
3.3. Effects of DL on Body Weight Changes and Food Intake
3.4. Effects of DL on Zymosan-Induced Colonic Inflammation
3.5. Effects of DL on Visceral Pain-Related Behaviors
3.6. Effects of DL on Anxiety-Like Behaviors
3.7. Effects of DL on c-Fos Protein Expression in the Brain
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group | Length Score | Weight Score | Stool Score | Macroscopic Score |
---|---|---|---|---|
Naïve | 0.20 ± 0.40 | 0.00 ± 0.00 | 0.60 ± 0.58 | 0.80 ± 0.86 |
Control | 1.40 ± 0.50 ### | 3.25 ± 0.50 ### | 2.95 ± 0.84 ### | 6.95 ± 1.72 ### |
DL100 | 0.00 ± 0.00 *** | 1.25 ± 0.96 | 1.30 ± 0.69 ** | 2.30 ± 1.58 *** |
DL200 | 0.00 ± 0.00 *** | 1.00 ± 0.82 * | 1.00 ± 0.50 ** | 1.80 ± 1.04 *** |
DL400 | 0.00 ± 0.00 *** | 1.25 ± 0.96 | 1.25 ± 0.73 ** | 2.25 ± 1.24 *** |
AMT | 0.00 ± 0.00 *** | 2.25 ± 0.96 | 1.45 ± 0.82 * | 3.25 ± 1.79 ** |
SSZ | 0.40 ± 0.50 ** | 1.00 ± 0.82 * | 1.45 ± 0.41 * | 2.65 ± 1.42 *** |
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Chun, E.; Yoon, S.; Parveen, A.; Jin, M. Alleviation of Irritable Bowel Syndrome-Like Symptoms and Control of Gut and Brain Responses with Oral Administration of Dolichos lablab L. in a Mouse Model. Nutrients 2018, 10, 1475. https://doi.org/10.3390/nu10101475
Chun E, Yoon S, Parveen A, Jin M. Alleviation of Irritable Bowel Syndrome-Like Symptoms and Control of Gut and Brain Responses with Oral Administration of Dolichos lablab L. in a Mouse Model. Nutrients. 2018; 10(10):1475. https://doi.org/10.3390/nu10101475
Chicago/Turabian StyleChun, Eunho, Soojung Yoon, Amna Parveen, and Mirim Jin. 2018. "Alleviation of Irritable Bowel Syndrome-Like Symptoms and Control of Gut and Brain Responses with Oral Administration of Dolichos lablab L. in a Mouse Model" Nutrients 10, no. 10: 1475. https://doi.org/10.3390/nu10101475