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Article

Discriminant Value of %Microcytic Cells/%Hypochromic Cells Ratio in the Differential Diagnosis of Microcytic Anemia

by
Eloísa Urrechaga
Haematology Laboratory, Hospital Galdakao—Usansolo, 48960 Galdakao, Vizcaya, Spain
Thalass. Rep. 2020, 10(1), 8388; https://doi.org/10.4081/thal.2020.8388
Submission received: 11 July 2019 / Revised: 7 December 2019 / Accepted: 17 December 2019 / Published: 3 March 2020

Abstract

The Mindray 6800 Plus analyzer reports red cells (RBC) extended parameters, which represent the subsets of erythrocytes. We aimed to evaluate the reliability of RBC extended parameters in the differential diagnosis of microcytic anemia. The learning set comprised samples from 250 patients with microcytic anemia mean cell volume <80 fL. MH ratio (%microcytic cells/%hypochromic cells) and other discriminant functions were calculated. Optimal cut offs were established using receiver operator curves. This value was used in the validation set of 135 patients 50 carriers and 85 with mild iron deficiency anemia (IDA). Area under the curve 0.945 (95% confidence interval 0.890 to 0.977), cut off >10 rendered the best Youden index (0.798), sensitivity 93.2%, specificity 86.2%. In the validation set using MH ratio >10, 45 in 50 patients were correctly classified as carriers. All of 40 beta carriers were correctly classified, while the 5 false negatives resulted to be alpha carriers. In the IDA group 5 patients had MH ratio >10 and thus considered carriers, but all of them had Hyper <3%. The combination of MH ratio >10 and %Hyper <3% correctly classified 100% of IDA patients. An algorithm derived from RBC extended parameters provided by the Mindray 6800 Plus analyzer could be a useful tool in the differential diagnosis of microcytic anemia.
Keywords: anemia; discriminant formulae; iron deficiency; thalassemia anemia; discriminant formulae; iron deficiency; thalassemia

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MDPI and ACS Style

Urrechaga, E. Discriminant Value of %Microcytic Cells/%Hypochromic Cells Ratio in the Differential Diagnosis of Microcytic Anemia. Thalass. Rep. 2020, 10, 8388. https://doi.org/10.4081/thal.2020.8388

AMA Style

Urrechaga E. Discriminant Value of %Microcytic Cells/%Hypochromic Cells Ratio in the Differential Diagnosis of Microcytic Anemia. Thalassemia Reports. 2020; 10(1):8388. https://doi.org/10.4081/thal.2020.8388

Chicago/Turabian Style

Urrechaga, Eloísa. 2020. "Discriminant Value of %Microcytic Cells/%Hypochromic Cells Ratio in the Differential Diagnosis of Microcytic Anemia" Thalassemia Reports 10, no. 1: 8388. https://doi.org/10.4081/thal.2020.8388

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