Bone Morphogenic Protein 2-Loaded Porous Silicon Carriers for Osteoinductive Implants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fabrication, Chemical Modification and Chracterization of PSiO2 Carriers
2.2. BMP-2 Loading and Release from PSiO2 Carriers
2.3. Isolation and Culture of Mesenchymal Stem Cells (MSCs) from Rabbit Bone Marrow (BM)
2.4. Cell Viability Assay
2.5. In Vitro BMP-2 Bioactivity Assay
2.6. Integration of the PSiO2 Carriers into a 3D-Printed Scaffold for Critical Size Bone Defects
3. Results
3.1. Fabrication and Chemical Modifications of PSiO2 Carriers
3.2. BMP-2 Loading and Release from PSiO2 Carriers
3.3. Cell Viability Studies for Proving the Biocompatibility of the PSiO2 Carriers.
3.4. Evaluation of the Bioactivity of the Released BMP-2
3.5. Integration of the PSiO2 Carriers into a 3D-Printed Scaffold for Critical Size Bone Defects
4. Discussion
4.1. BMP-2 Loading and Release from PSiO2 Carriers
4.2. Cell Viability and Bioactivity of the Released BMP-2
4.3. Integration of the PSiO2 Carriers into a 3D-Printed Scaffold for Critical Size Bone Defects
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Rosenberg, M.; Shilo, D.; Galperin, L.; Capucha, T.; Tarabieh, K.; Rachmiel, A.; Segal, E. Bone Morphogenic Protein 2-Loaded Porous Silicon Carriers for Osteoinductive Implants. Pharmaceutics 2019, 11, 602. https://doi.org/10.3390/pharmaceutics11110602
Rosenberg M, Shilo D, Galperin L, Capucha T, Tarabieh K, Rachmiel A, Segal E. Bone Morphogenic Protein 2-Loaded Porous Silicon Carriers for Osteoinductive Implants. Pharmaceutics. 2019; 11(11):602. https://doi.org/10.3390/pharmaceutics11110602
Chicago/Turabian StyleRosenberg, Michal, Dekel Shilo, Leonid Galperin, Tal Capucha, Karim Tarabieh, Adi Rachmiel, and Ester Segal. 2019. "Bone Morphogenic Protein 2-Loaded Porous Silicon Carriers for Osteoinductive Implants" Pharmaceutics 11, no. 11: 602. https://doi.org/10.3390/pharmaceutics11110602