Detecting the Different Responses of Roots and Shoots to Gravity in Salix matsudana (Koidz)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Tissue Collection
2.2. RNA Sequencing and Library Construction
2.3. Analysis of the Sequencing Data
2.4. Identification of Differentially Expressed Genes
2.5. Analysis of the Alternative Splicing Genes under Different Conditions
2.6. Construction of a Protein Interaction Network
2.7. qRT-PCR Analysis of the Gravity-Related Genes
3. Results
3.1. Overview of the RNA Sequencing Data
3.2. The Differentially Expressed Genes between the Roots and Shoots under Normal and Inverted Conditions
3.3. Construction of Gravity-Related Gene Expression Networks in the Roots and Shoots
3.4. Analysis of the Gravity-Induced Alternative Splicing Genes in S. matsudana
3.5. Expression Profiling of the Gravity-Related Genes
4. Discussion
4.1. The Difference in the Gravity Response between the Roots and Shoots of S. matsudana
4.2. A Potential Functional Network of the Genes Associated with Gravity
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, G.; Li, Y.; Gao, J.; Feng, Z.; Guo, H.; Zou, H.; Chu, X.; Li, Y.; Chen, Y.; Yu, C.; et al. Detecting the Different Responses of Roots and Shoots to Gravity in Salix matsudana (Koidz). Forests 2021, 12, 1715. https://doi.org/10.3390/f12121715
Liu G, Li Y, Gao J, Feng Z, Guo H, Zou H, Chu X, Li Y, Chen Y, Yu C, et al. Detecting the Different Responses of Roots and Shoots to Gravity in Salix matsudana (Koidz). Forests. 2021; 12(12):1715. https://doi.org/10.3390/f12121715
Chicago/Turabian StyleLiu, Guoyuan, Yixin Li, Junfeng Gao, Zhicong Feng, Hongyi Guo, Hang Zou, Xintong Chu, Yaqi Li, Yanhong Chen, Chunmei Yu, and et al. 2021. "Detecting the Different Responses of Roots and Shoots to Gravity in Salix matsudana (Koidz)" Forests 12, no. 12: 1715. https://doi.org/10.3390/f12121715