Impact of Activation of EGFL7 within Microenvironment of High Grade Ovarian Serous Carcinoma on Infiltration of CD4+ and CD8+ Lymphocytes
, Anton Żawrocki 2, Natalia Krawczyńska 3,4, Michał Bieńkowski 2, Bartosz Wasąg 3,4 and Wojciech Biernat 2Round 1
Reviewer 1 Report
The authors describe the association of EGFL7 expression with the expression of ICAM-1 as well as the abundance of CD4+/CD8+ lymphocytes. The study subject was high-grade ovarian serous carcinomas. The specimen used were FFPE tissue samples from primary debulking surgeries.
As EGFL7 expression is an important, though prognosis independent, marker for higher tumor grade in other cancers types, this study provides an insight whether or not this could be the case for HGOSC.
The study methodology is sound and well thought out. The authors' description directly addresses the weaknesses and potential of the study as well as a sound conclusion as to why the association of Egfl7 with CD4 and CD8 lymphocytes is important. Further research into the immune evasion of ovarian cancer is needed, not only in regard to EGFL7.
If possible, and that might very well be outside of the scope of the research possible, results on a bigger study cohort would be interesting as well as the observation of other immune markers in HGOSC.
Author Response
Thank You
Reviewer 2 Report
Detailing the working method and the conclusions would be particularly useful for the public.
Author Response
Description of methodology was approved
