Flaxseed Ethanol Extract Effect in Acute Experimental Inflammation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Plant Extract Preparation
2.3. Experimental Design
2.3.1. Animal Subjects
2.3.2. Experimental Protocol
2.3.3. In Vivo Anti-inflammatory and Oxidative Stress Markers
2.3.4. Statistical Analysis
3. Results
In Vivo Anti-Inflammatory and Antioxidative Effect
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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NOx (ng/mL) | 3NT (ng/mL) | NFkB (ng/mL) | ||||
---|---|---|---|---|---|---|
CONTROL | 39.355 | ±4.609 | 2.169 | ±0.282 | 28.429 | ±3.937 |
INFAM | 53.555 | ±5.346 ### | 6.510 | ±1.475 ### | 76.131 | ±8.612 ### |
FLAX100/INFAM | 35.701 | ±3.871 *** | 3.506 | ±1.095 *** | 39.448 | ±2.426 *** |
FLAX50/INFAM | 37.824 | ±6.201 ** | 3.728 | ±0.997 *** | 50.934 | ±3.070 ** |
FLAX25/INFAM | 39.234 | ±8.150 ** | 4.384 | ±1.869 * | 52.539 | ±8.323 * |
INFLAM/FLAX100 | 27.108 | ±6.554 *** | 3.420 | ±1.059 *** | 26.841 | ±1.208 *** |
INFLAM/FLAX50 | 29.464 | ±4.081 *** | 3.510 | ±0.989 *** | 37.885 | ±3.355 *** |
INFLAM/FLAX25 | 31.788 | ±5.157 *** | 3.977 | ±1.278 *** | 39.153 | ±2.840 *** |
INFAM/DICLO | 31.550 | ±7.284 *** | 4.510 | ±2.003 ** | 40.771 | ±4.603 ** |
TAC (mmol Troloxequiv/L) | TOS (mmol Troloxequiv/L) | OSI | MDA (nM/L) | SH (mM/L) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
CONTROL | 1.093 | ±0.001 | 5.181 | ±0.899 | 5.157 | ±0.626 | 2.496 | ±0.258 | 442.000 | ±45.778 |
INFAM | 1.088 | ±0.001 ## | 6.798 | ±0.712 ### | 6.570 | ±0.621 ### | 4.310 | ±0.130 ### | 273.800 | ±35.654 ### |
FLAX100/INFAM | 1.094 | ±0.002 *** | 5.368 | ±0.710 ** | 4.662 | ±1.432 *** | 2.893 | ±0.354 *** | 420.500 | ±51.675 *** |
FLAX50/INFAM | 1.092 | ±0.001 ** | 5.546 | ±1.085 ** | 5.061 | ±0.604 ** | 3.238 | ±0.411 ** | 313.000 | ±35.515 ** |
FLAX25/INFAM | 1.091 | ±0.002 ** | 6.387 | ±0.709 | 5.269 | ±0.374 * | 3.248 | ±0.245 ** | 289.333 | ±80.353 |
INFLAM/FLAX100 | 1.090 | ±0.002 ** | 4.008 | ±0.412 *** | 3.633 | ±0.856 *** | 3.173 | ±0.764 ** | 357.000 | ±28.107 *** |
INFLAM/FLAX50 | 1.089 | ±0.002 * | 4.295 | ±0.533 *** | 4.128 | ±0.662 *** | 3.364 | ±0.522 ** | 313.800 | ±35.344 ** |
INFLAM/FLAX25 | 1.091 | ±0.001 ** | 6.224 | ±0.819 | 5.749 | ±0.405 * | 3.931 | ±0.215 | 287.400 | ±34.392 |
INFAM/DICLO | 1.095 | ±0.002 *** | 5.807 | ±0.586 ** | 5.272 | ±0.724 * | 3.285 | ±0.262 ** | 360.000 | ±40.214 ** |
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Chera, E.I.; Pop, T.I.; Pop, R.M.; Pârvu, M.; Uifălean, A.; Cătoi, F.A.; Cecan, A.D.; Mîrza, C.M.; Achimaș-Cadariu, P.; Pârvu, A.E. Flaxseed Ethanol Extract Effect in Acute Experimental Inflammation. Medicina 2022, 58, 582. https://doi.org/10.3390/medicina58050582
Chera EI, Pop TI, Pop RM, Pârvu M, Uifălean A, Cătoi FA, Cecan AD, Mîrza CM, Achimaș-Cadariu P, Pârvu AE. Flaxseed Ethanol Extract Effect in Acute Experimental Inflammation. Medicina. 2022; 58(5):582. https://doi.org/10.3390/medicina58050582
Chicago/Turabian StyleChera, Elisabeta Ioana, Tiberia Ioana Pop, Raluca Maria Pop, Marcel Pârvu, Ana Uifălean, Florinela Adriana Cătoi, Andra Diana Cecan, Camelia Manuela Mîrza, Patriciu Achimaș-Cadariu, and Alina Elena Pârvu. 2022. "Flaxseed Ethanol Extract Effect in Acute Experimental Inflammation" Medicina 58, no. 5: 582. https://doi.org/10.3390/medicina58050582