Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients’ Sera
2.2. Preparation of Artemisia spp. Pollen Extracts
2.3. Protein Gel Electrophoresis and Immunoblotting
2.4. Protein Purification
2.5. Mass Spectrometry and N-Terminal Sequencing
2.6. cDNA Cloning
2.7. ELISA and Cross-Inhibition Studies
2.8. Mediator Release Assay
2.9. T Cell Reactivity
2.10. ISAC Inhibition Studies
2.11. Statistics
3. Results
3.1. Geographic Distribution of Artemisia spp. and Analysis of Pollen Proteins
3.2. IgE Binding Capacity and Art v 1 Cross-Reactivity Pattern of Artemisia Extracts
3.3. Purification of DPLPs
3.4. cDNA Cloning and Amino Acid Sequence Identification
3.5. IgE Reactivity to Purified DPLPs
3.6. Mediator Release Assay
3.7. Art v 1 and DPLPs Presented T cell Cross-Reactivity
3.8. IgE Cross-Reactivity within Other Allergenic Protein Families
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Latin Name | Common Name | Special Use |
---|---|---|
A. absinthium | absinthe wormwood, wormwood, green ginger, or grand wormwood | medicinal herb, ingredient of the liqueur “absinthe” |
A. annua | sweet wormwood, sweet sage wort, or annual wormwood | traditional Chinese herbal medicine; leaves are used for antimalaria treatment (artemisinin) |
A. californica | California sagebrush | spices and tea |
A. frigida | sagebrush, prairie sage wort, or fringed wormwood | medicinal herb containing camphor |
A. ludoviciana | silver wormwood or white sagebrush | medicinal herb |
A. tridentata | common sagebrush or mountain sagebrush | traditional medicine of North American Indian tribes |
A. vulgaris | mugwort or common wormwood | traditional Chinese herbal medicine |
% MI (SD) 0.005 µg/mL nArt v 1 | % MI (SD) 0.05 µg/mL nArt v 1 | % MI (SD) 0.5 µg/mL nArt v 1 | % MI (SD) 5.0 µg/mL nArt v 1 | |
---|---|---|---|---|
A. absinthium | 70.4 (20.4) | 75.8 (17.9) | 80.5 (11.7) | 85.3 (6.8) |
A. annua | 75.2 (17.5) | 81.5 (16.3) | 85.2 (9.3) | 90.2 (5.8) |
A. californica | 72.8 (15.6) | 80.0 (15.1) | 90.5 (8.4) | 90.5 (5.2) |
A. frigida | 74.3 (16.8) | 82.5 (12.7) | 86.7 (7.2) | 90.2 (5.4) |
A. ludoviciana | 69.5 (21.2) | 75.6 (19.4) | 81.7 (10.3) | 87.3 (6.2) |
A. tridentata | 73.9 (17.0) | 80.0 (14.9) | 84.1 (10.9) | 87.2 (8.0) |
A. vulgaris | 63.8 (21.2) | 76.4 (17.9) | 78.6 (10.6) | 82.1 (6.6) |
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Pablos, I.; Egger, M.; Vejvar, E.; Reichl, V.; Briza, P.; Zennaro, D.; Rafaiani, C.; Pickl, W.; Bohle, B.; Mari, A.; et al. Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules. Medicina 2019, 55, 504. https://doi.org/10.3390/medicina55080504
Pablos I, Egger M, Vejvar E, Reichl V, Briza P, Zennaro D, Rafaiani C, Pickl W, Bohle B, Mari A, et al. Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules. Medicina. 2019; 55(8):504. https://doi.org/10.3390/medicina55080504
Chicago/Turabian StylePablos, Isabel, Matthias Egger, Eva Vejvar, Victoria Reichl, Peter Briza, Danila Zennaro, Chiara Rafaiani, Winfried Pickl, Barbara Bohle, Adriano Mari, and et al. 2019. "Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules" Medicina 55, no. 8: 504. https://doi.org/10.3390/medicina55080504