An In Vitro and In Vivo Study on the Intensity of Adhesion and Colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on Originally Synthesized Biomaterials With Different Chemical Composition and Modified Surfaces and Their Effect on Expression of TNF-α, β-Defensin 2 and IL-10 in Tissues
Abstract
Materials and Methods. Biomaterial discs were contaminated with bacterial suspensions of 10, 102, and 103 colony forming units (CFU)/mL (P. aeruginosa ATCC 27853 and S. epidermidis ATCC 12228), and after 2 hours of cultivation, the intensity of bacterial adhesion was determined. For in vivo tests, the samples were contaminated with 102 and 103 CFU/mL cultivated at 37oC for 2 h to ensure bacterial adhesion. Contaminated biomaterial samples were implanted in the interscapular area of chinchilla rabbits for 2 and 4 weeks. The biomaterials were removed, and using plate count and sonification methods, bacterial colonization on the surface of biomaterials was determined. Moreover, the expression of TNF-α, β-defensin 2, and IL-10 in the surrounding tissues was assessed by using immunohistochemistry methods.
Results. P. aeruginosa more intensively colonized biomaterials in the in vivo study as compared with S. epidermidis. Il-10 is a regulatory cytokine, which reduces the intensity of inflammatory cell activity, thus reducing nonspecific resistance of the organism.
Conclusions. The expression of TNF-α and IL-10 was not affected by short (2 and 4 weeks) biomaterial implantation. Pronounced cytokine expression in tissues around implanted biomaterials contaminated with P. aeruginosa was observed.
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Reinis, A.; Pilmane, M.; Stunda, A.; Vētra, J.; Kroiča, J.; Rostoka, D.; Šalms, Ģ.; Vostroilovs, A.; Dons, A.; Bērziņa-Cimdiņa, L. An In Vitro and In Vivo Study on the Intensity of Adhesion and Colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on Originally Synthesized Biomaterials With Different Chemical Composition and Modified Surfaces and Their Effect on Expression of TNF-α, β-Defensin 2 and IL-10 in Tissues. Medicina 2011, 47, 80. https://doi.org/10.3390/medicina47100080
Reinis A, Pilmane M, Stunda A, Vētra J, Kroiča J, Rostoka D, Šalms Ģ, Vostroilovs A, Dons A, Bērziņa-Cimdiņa L. An In Vitro and In Vivo Study on the Intensity of Adhesion and Colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on Originally Synthesized Biomaterials With Different Chemical Composition and Modified Surfaces and Their Effect on Expression of TNF-α, β-Defensin 2 and IL-10 in Tissues. Medicina. 2011; 47(10):80. https://doi.org/10.3390/medicina47100080
Chicago/Turabian StyleReinis, Aigars, Māra Pilmane, Agnese Stunda, Jānis Vētra, Juta Kroiča, Dagnija Rostoka, Ģirts Šalms, Antons Vostroilovs, Aleksejs Dons, and Līga Bērziņa-Cimdiņa. 2011. "An In Vitro and In Vivo Study on the Intensity of Adhesion and Colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on Originally Synthesized Biomaterials With Different Chemical Composition and Modified Surfaces and Their Effect on Expression of TNF-α, β-Defensin 2 and IL-10 in Tissues" Medicina 47, no. 10: 80. https://doi.org/10.3390/medicina47100080
