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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.

Curr. Issues Mol. Biol., Volume 4, Issue 1 (January 2002) – 3 articles

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846 KiB  
Review
Advances Towards Integrated Biodetection Systems for Environmental Molecular Microbiology
by Darrell P. Chandler
Curr. Issues Mol. Biol. 2002, 4(1), 19-32; https://doi.org/10.21775/cimb.004.019 - 13 Feb 2002
Viewed by 393
Abstract
To overcome many of the limitations associated with indirect detection methods, new techniques for the sensitive, specific, and direct detection of nucleic acids are required in order to accurately and quantitatively ascribe phenotype/function to uncultivated microorganisms. However, if advanced diagnostic and detection systems [...] Read more.
To overcome many of the limitations associated with indirect detection methods, new techniques for the sensitive, specific, and direct detection of nucleic acids are required in order to accurately and quantitatively ascribe phenotype/function to uncultivated microorganisms. However, if advanced diagnostic and detection systems are going to be applied in environmental microbiology, future "biodetection" technologies and systems must be developed not from the point of view of the detector, but from the unique aspects of the environmental sample and the entire analytical process. This article highlights recent advances in nucleic acid-based technologies, and look towards future advances that may address the broad needs and conditions imposed by environmental molecular microbiology. Full article
588 KiB  
Review
Analysis of Specific Bacteria from Environmental Samples Using a Quantitative Polymerase Chain Reaction
by Clifford F. Brunk, Jinliang Li and Erik Avaniss-Aghajani
Curr. Issues Mol. Biol. 2002, 4(1), 13-18; https://doi.org/10.21775/cimb.004.013 - 13 Feb 2002
Cited by 1 | Viewed by 428
Abstract
This article describes the use of quantitative PCR for measuring bacterial abundance in environmental samples. The two approaches discussed are: (1) The use of an internal PCR standard constructed to be the same size and have the same sequence as the primary amplification [...] Read more.
This article describes the use of quantitative PCR for measuring bacterial abundance in environmental samples. The two approaches discussed are: (1) The use of an internal PCR standard constructed to be the same size and have the same sequence as the primary amplification target, but differing from the primary target by 2–3 bases, corresponding to a unique restriction site. This allows the amount of target amplicon to be compared with the internal standard and circumvents the problem of differential amplification efficiencies when using dissimilar targets and standard amplicons. (2) The use of Taqman technology (Applied Biosystems, Foster City, California) with a dual labeled oligonucleotide probe which binds internal to the PCR primers. The detection of Bacteroides is used as an example for both approaches. Full article
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Review
The Molecular Evolution and DNA Profiling of Toxic Cyanobacteria
by Brett A. Neilan
Curr. Issues Mol. Biol. 2002, 4(1), 1-11; https://doi.org/10.21775/cimb.004.001 - 13 Feb 2002
Viewed by 406
Abstract
Rapid and sensitive methods for the detection and genetic characterization of cyanobacteria have been developed based on DNA amplification techniques. This article describes the molecular methods that have been used to characterize cyanobacteria and their use as tools to identify toxin-producing strains. Different [...] Read more.
Rapid and sensitive methods for the detection and genetic characterization of cyanobacteria have been developed based on DNA amplification techniques. This article describes the molecular methods that have been used to characterize cyanobacteria and their use as tools to identify toxin-producing strains. Different species and strains were compared using restriction fragment length polymorphism (RFLP) of amplified fragments of the phycocyanin gene and the 16S-23S rRNA internal transcribed spacer. Full article
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