Styryl Hemicyanine Dye (E)-3-Methyl-2-(4-thiomorpholinostyryl)benzo[d]thiazol-3-ium Iodide for Nucleic Acids and Cell Nucleoli Visualization
, Marina Miteva 2, Nikolay Ishkitiev 2, Maria Dragneva 2, Lora Topalova 2 and Meglena I. Kandinska 1,*
Round 1
Reviewer 1 Report
I recommend the following :
The main absorption bands to which functional groups are assigned?
Specified for the assignment of the main bands in the IR spectrum of compound 1 in which it was concretely recorded
The solvent used must be specified for chemical shifts of compound 1 from NMR spectra
Conclusions are not specified
Authors should check how the bibliographic notes are written
Author Response
Point 1: The main absorption bands to which functional groups are assigned? Specified for the assignment of the main bands in the IR spectrum of compound 1 in which it was concretely recorded.
Response 1: We are grateful to the Reviewer for the suggestion. The bands in the IR spectra of dye 1 were assigned according to the literature.
Point 2: The solvent used must be specified for chemical shifts of compound 1 from NMR spectra.
Response 2: On Line 131 was written: 1H-NMR δ =, now is corrected to: 1H-NMR (DMSO-d6, δ(ppm)).
Point 3: Conclusions are not specified.
Response 3: Thanks a lot for the suggestion! We introduced a section Conclusions in the revised manuscript.
Point 4: Authors should check how the bibliographic notes are written.
Response 4: Please, find in the revised draft of the manuscript the bibliographic notes arranged by us according to the requirements of the Molbank Journal.
Author Response File: 
Author Response.docx
Reviewer 2 Report
The manuscript "Styryl hemicyanine dye (E)-3-Methyl-2-(4-thiomorpholino- styryl)benzo[d]thiazol-3-ium iodide for nucleic acids and cell nucleoli visualization" describe a novel compound. I recommend the manuscript to be accepted to MolBank after minor revision:
1. Lines 124-125: the amount of piperidine should be given in mL.
2. In SI spectra plots should be bigger in size.
Author Response
Point 1: Lines 124-125: the amount of piperidine should be given in mL.
Response 1: Thank you very much for the note! The amount of piperidine in line 124-125 was defined to 0.03 mL.
Point 2. In SI spectra plots should be bigger in size.
Response 2: In SI all pictures and plots have been increased.
Author Response File: Author Response.docx
