Inhibitory Effects of Extracellular Vesicles from iPS-Cell-Derived Mesenchymal Stem Cells on the Onset of Sialadenitis in Sjögren’s Syndrome Are Mediated by Immunomodulatory Splenocytes and Improved by Inhibiting miR-125b
Abstract
:1. Introduction
2. Results
2.1. Systemically Infused iEVs Were Mainly Taken up by Macrophages in Spleen
2.2. Young iEVs but Not Aging iEVs Promoted M2 Polarization of Splenic Macrophages
2.3. Young iEVs but Not Aging iEVs Decreased Th17 Cells in Spleen
2.4. Inhibition of miR-125b in Aging iMSCs Enabled Their EVs to Repress the Onset of Sialadenitis
2.5. Inhibition of miR-125b in Aging iEVs Restored Their Activity to Promote M2 Polarization
2.6. Inhibition of miR-125b in Aging iEVs Improved Their Activity to Decrease Splenic Th17 Cells
3. Discussion
4. Materials and Methods
4.1. iMSC Culture
4.2. Isolation of iMSC-EVs and Characterization
4.3. Animal Studies
4.4. Flow Cytometry
4.5. Real-Time PCR Analysis of mRNAs and miRNAs
4.6. Cell Transfection
4.7. ELISA
4.8. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, Q.; Bae, E.-H.; Zhang, Y.; Shahsavari, A.; Lotey, P.; Lee, R.H.; Liu, F. Inhibitory Effects of Extracellular Vesicles from iPS-Cell-Derived Mesenchymal Stem Cells on the Onset of Sialadenitis in Sjögren’s Syndrome Are Mediated by Immunomodulatory Splenocytes and Improved by Inhibiting miR-125b. Int. J. Mol. Sci. 2023, 24, 5258. https://doi.org/10.3390/ijms24065258
Zhao Q, Bae E-H, Zhang Y, Shahsavari A, Lotey P, Lee RH, Liu F. Inhibitory Effects of Extracellular Vesicles from iPS-Cell-Derived Mesenchymal Stem Cells on the Onset of Sialadenitis in Sjögren’s Syndrome Are Mediated by Immunomodulatory Splenocytes and Improved by Inhibiting miR-125b. International Journal of Molecular Sciences. 2023; 24(6):5258. https://doi.org/10.3390/ijms24065258
Chicago/Turabian StyleZhao, Qingguo, Eun-Hye Bae, Yu Zhang, Arash Shahsavari, Pranayvir Lotey, Ryang Hwa Lee, and Fei Liu. 2023. "Inhibitory Effects of Extracellular Vesicles from iPS-Cell-Derived Mesenchymal Stem Cells on the Onset of Sialadenitis in Sjögren’s Syndrome Are Mediated by Immunomodulatory Splenocytes and Improved by Inhibiting miR-125b" International Journal of Molecular Sciences 24, no. 6: 5258. https://doi.org/10.3390/ijms24065258