The Utility of Noninvasive Urinary Biomarkers for the Evaluation of Vesicoureteral Reflux in Children
Abstract
:1. Introduction
2. Results
2.1. Patient Characteristics
2.2. Urinary IL-6, NGAL, and LL-37 Concentrations
2.3. Results Regarding RS, RN, and CKD
2.4. ROC Curve Analysis
3. Discussion
Authors | Year | Description | Results |
---|---|---|---|
Ninan et al. [25] | 1999 | 32 children: 11 with VUR and RN, 6 with VUR, and 15 controls | No differences between patients with VUR and the controls. A significant increase in IL-6 levels was observed in patients with RN. |
Haraoka et al. [26] | 1996 | 32 children with VUR without UTI | No differences between patients with VUR and controls. Urinary IL-6 levels were below the lower limit of detection (10 pg/mL). |
Cordoba et al. [27] | 2012 | Case-control study on 40 patients who underwent a VCUG | No differences between patients with VUR and controls. |
Grupta et al. [30] | 2020 | 23 patients with a history of febrile UTI and RS and 27 patients with a history of febrile UTI without RS | No differences in urinary IL-6 levels between the two groups. |
Sheu et al. [28] | 2009 | 45 children (aged between 1 and 120 months) with acute pyelonephritis and 34 with lower UTI | No differences between patients with VUR and controls. Elevations in urinary IL-6 levels were correlated with an increased risk of subsequent RS. |
Tramma et al. [29] | 2012 | 23 children with VUR and RS, 10 children with RS without VUR, 4 children with VUR without RS, and 13 controls | No differences between patients with VUR and controls. Levels of urinary IL-6 were correlated with the severity of RS. |
Wang et al. [24] | 2001 | 66 children with RS | Strong correlation between RS and urinary IL-6 levels. |
Nickavar et al. [13] | 2020 | 70 children (34 with VUR, 36 controls) | Significantly higher urinary IL-6 levels in patients with VUR. No correlation between urinary IL-6 and the severity of VUR or RS. |
Gokce et al. [12] | 2010 | 114 children (26 with VUR and RS, 27 with VUR without RS, 34 with RS without VUR, and 27 controls) | Significantly higher urinary IL-6 levels in patients with VUR (poor diagnostic test—AUC of 0.64). Weak correlation between IL-6 and the grade of the VUR. No differences between patients with RS and controls. |
Krzemien et al. [11] | 2004 | 33 children aged 1–24 months with first-time UTI | Higher urinary IL-6 concentrations in patients with VUR. |
4. Materials and Methods
4.1. Patient Enrolment
4.2. Study Procedures
4.3. Urinary Markers Assay
4.4. Data Analysis and Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Characteristics | Patients (Median) | Controls (Median) | Patients Range | Controls Range | p Value |
---|---|---|---|---|---|
Age (years) | 3.91 | 5.83 | 0.33–16.33 | 0.04–17.00 | 0.62 |
W/H Z score | 0.07 | 0.15 | −3.12–2.18 | −7.30–2.00 | 0.87 |
eGFR (ml/min/1.73 m2) | 109 | 117 | 42.00–170.00 | 90.00–179.00 | 0.07 |
Serum creatinine (mg/dL) | 0.37 | 0.37 | 0.12–1.18 | 0.19–0.70 | 0.70 |
Urinary creatinine (mg/dL) | 40.19 | 43.95 | 1.13–172.92 | 2.65–254.62 | 0.99 |
uACR | 0.01 | 0.008 | 0.002–1.40 | 0.004–0.47 | 0.73 |
IL-6 (pg/mL) | 1.83 | 1.75 | 1.33–135.10 | 1.33–3.15 | 0.14 |
IL-6/creatinine (pg/mg) | 0.06 | 0.05 | 0.01–16.89 | 0.009–0.63 | 0.22 |
LL-37 (ng/mL) | 0.85 | 0.84 | 0.71–3.03 | 0.68–1.11 | 0.21 |
LL-37/creatinine (ng/mg) | 0.02 | 0.01 | 0.005–0.79 | 0.003–0.33 | 0.65 |
NGAL (pg/mL) | 2755 | 2297 | 20.60–10,000 | 23.00–3115 | 0.02 |
NGAL/creatinine (pg/mg) | 64.19 | 40.15 | 8.01–1075.26 | 0.76–963.77 | 0.05 |
NGAL/LL-37 (pg/ng) | 3125 | 2744 | 23.15–14,084 | 29.11–4061.33 | 0.03 |
IL-6/LL-37 (pg/ng) | 2.24 | 2.07 | 0.55–168.87 | 1.36–3.86 | 0.61 |
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Colceriu, M.-C.; Aldea, P.L.; Boț, A.-L.; Bulată, B.; Delean, D.; Grama, A.; Mititelu, A.; Decea, R.M.; Sevastre-Berghian, A.; Clichici, S.; et al. The Utility of Noninvasive Urinary Biomarkers for the Evaluation of Vesicoureteral Reflux in Children. Int. J. Mol. Sci. 2023, 24, 17579. https://doi.org/10.3390/ijms242417579
Colceriu M-C, Aldea PL, Boț A-L, Bulată B, Delean D, Grama A, Mititelu A, Decea RM, Sevastre-Berghian A, Clichici S, et al. The Utility of Noninvasive Urinary Biomarkers for the Evaluation of Vesicoureteral Reflux in Children. International Journal of Molecular Sciences. 2023; 24(24):17579. https://doi.org/10.3390/ijms242417579
Chicago/Turabian StyleColceriu, Marius-Cosmin, Paul Luchian Aldea, Andreea-Liana Boț (Răchişan), Bogdan Bulată, Dan Delean, Alina Grama, Alexandra Mititelu, Roxana Maria Decea, Alexandra Sevastre-Berghian, Simona Clichici, and et al. 2023. "The Utility of Noninvasive Urinary Biomarkers for the Evaluation of Vesicoureteral Reflux in Children" International Journal of Molecular Sciences 24, no. 24: 17579. https://doi.org/10.3390/ijms242417579