Revealing the Mechanisms of Enhanced β-Farnesene Production in Yarrowia lipolytica through Metabolomics Analysis
Abstract
:1. Introduction
2. Results and Discussion
2.1. The Impact of Amino Acids/Nucleobases Supplementation on β-Farnesene Biosynthesis
2.2. Metabolomics Analysis and Localization of Differential Metabolites
2.3. Gene Editing and Culture Medium Optimization Based on Metabolomic Analysis
2.4. Fed-Batch Fermentation in a 30-Liter Fermenter
3. Materials and Methods
3.1. Plasmids and Strains Construction
3.2. Shake Flask Fermentation
3.3. Fed-Batch Fermentation
3.4. Metabolite Extraction and LC-QTOF-MS Detection
3.5. LC-MS Data Processing
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Substrate | Fermentation Scale | Titer (g/L) | Yield (g/g) | Reference |
---|---|---|---|---|
Glucose | 2-L fermenter | 28.9 | 0.0855 | [14] |
Lignocellulosic hydrolysate | 2-L fermenter | 7.38 | 0.075 | [15] |
Oleic acid | 5-L fermenter | 35.2 | 0.170 | [16] |
Acetate | 5-L fermenter | 14.8 | 0.0558 | [17] |
Lysine | Proline | Serine | Uracil | Valine | |
---|---|---|---|---|---|
Upregulated | 701 | 563 | 689 | 660 | 340 |
Downregulated | 666 | 423 | 415 | 435 | 227 |
Total | 1367 | 986 | 1104 | 1095 | 567 |
Time (Min) | Pump A (%) | Pump B (%) | Flow (mL/Min) | Max Pressure Limit (Bar) |
---|---|---|---|---|
0.00 | 3.00 | 97.00 | 0.30 | 600.00 |
1.00 | 3.00 | 97.00 | 0.30 | 600.00 |
10.00 | 15.00 | 85.00 | 0.30 | 600.00 |
15.00 | 28.00 | 72.00 | 0.30 | 600.00 |
20.00 | 60.00 | 40.00 | 0.30 | 600.00 |
25.00 | 60.00 | 40.00 | 0.30 | 600.00 |
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Liu, Q.; Bi, H.; Wang, K.; Zhang, Y.; Chen, B.; Zhang, H.; Wang, M.; Fang, Y. Revealing the Mechanisms of Enhanced β-Farnesene Production in Yarrowia lipolytica through Metabolomics Analysis. Int. J. Mol. Sci. 2023, 24, 17366. https://doi.org/10.3390/ijms242417366
Liu Q, Bi H, Wang K, Zhang Y, Chen B, Zhang H, Wang M, Fang Y. Revealing the Mechanisms of Enhanced β-Farnesene Production in Yarrowia lipolytica through Metabolomics Analysis. International Journal of Molecular Sciences. 2023; 24(24):17366. https://doi.org/10.3390/ijms242417366
Chicago/Turabian StyleLiu, Qianxi, Haoran Bi, Kai Wang, Yang Zhang, Biqiang Chen, Huili Zhang, Meng Wang, and Yunming Fang. 2023. "Revealing the Mechanisms of Enhanced β-Farnesene Production in Yarrowia lipolytica through Metabolomics Analysis" International Journal of Molecular Sciences 24, no. 24: 17366. https://doi.org/10.3390/ijms242417366