Molecular Mechanism of Cold Tolerance of Centipedegrass Based on the Transcriptome
Abstract
:1. Introduction
2. Results
2.1. Physiological Changes in Centipedegrass under Low-Temperature Stress
2.2. RNA-Seq Results
2.3. Functional Annotation of Isoforms
2.4. Long Noncoding RNA Prediction and Transcription Factor Identification
2.5. Differentially Expressed Genes (DEGs) in Response to Low-Temperature Stress
2.6. GO and KEGG Enrichment Analysis of DEGs
2.7. Arginine and Proline Metabolism
2.8. Flavonoid Biosynthesis
2.9. Plant Circadian Rhythm Pathway
2.10. Plant hormone Signal Transduction
2.11. Transcription Factors (TFs) and Transcriptional Regulation Factors (TRs)
2.12. Weighted Gene Coexpression Network Analysis (WGCNA)
2.13. qRT-PCR Validation of the DEGs
3. Discussion
3.1. Signal Transduction
3.2. Arginine Metabolism
3.3. Circadian Rhythm in Plants
3.4. Flavonoid Biosynthesis
3.5. Transcription Factors (TFs) and Transcriptional Regulation Factors (TRs)
4. Materials and Methods
4.1. Plant Materials and Low-Temperature Treatment
4.2. RNA Extraction, Library Construction and Sequencing
4.3. De Novo Assembly
4.4. Expression Calculation and Differential Expression Analysis
4.5. Functional Annotation of Transcripts
4.6. Quantitative Real-Time PCR (qRT–PCR) Analysis
4.7. WGCNA Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | TML |
---|---|
Subreads base (G) | 38.76 |
Subreads number | 16,244,578 |
Average subreads length | 2386 |
CCS | 331,513 |
FLNC | 281,066 |
Average FLNC read length | 2652 |
FLNC/CCS | 0.84 |
Total nucleotides | 349,159,670 |
Total number | 127,142 |
Mean length | 2746 |
Max length | 13,081 |
N50 | 3089 |
N90 | 1773 |
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Liu, Y.; Xiong, Y.; Zhao, J.; Bai, S.; Li, D.; Chen, L.; Feng, J.; Li, Y.; Ma, X.; Zhang, J. Molecular Mechanism of Cold Tolerance of Centipedegrass Based on the Transcriptome. Int. J. Mol. Sci. 2023, 24, 1265. https://doi.org/10.3390/ijms24021265
Liu Y, Xiong Y, Zhao J, Bai S, Li D, Chen L, Feng J, Li Y, Ma X, Zhang J. Molecular Mechanism of Cold Tolerance of Centipedegrass Based on the Transcriptome. International Journal of Molecular Sciences. 2023; 24(2):1265. https://doi.org/10.3390/ijms24021265
Chicago/Turabian StyleLiu, Yingjie, Yi Xiong, Junming Zhao, Shiqie Bai, Daxu Li, Limin Chen, Junjie Feng, Yingzhu Li, Xiao Ma, and Jianbo Zhang. 2023. "Molecular Mechanism of Cold Tolerance of Centipedegrass Based on the Transcriptome" International Journal of Molecular Sciences 24, no. 2: 1265. https://doi.org/10.3390/ijms24021265