Integrated Analysis of the Transcriptome and Metabolome of Brassica rapa Revealed Regulatory Mechanism under Heat Stress
Abstract
:1. Introduction
2. Result
2.1. Plant Growth and Physiological Activities of Brassica rapa under Heat Stress
2.2. Transcriptome Profiles of Brassica rapa in Response to Heat Stress
2.3. COG, GO and KEGG Pathway Analysis of DEGs
2.4. qRT–PCR Verification of RNA–Seq
2.5. Metabolic Profiles Analysis of Brassica rapa in Response to Heat Stress
2.6. Metabolic KEGG Pathways Analysis
3. Discussion
3.1. The Role of Heat Shock Protein Family in Response to Heat Stress
3.2. Plant Hormone Transduction Signaling in Response to Heat Stress
3.3. The Photosynthetic Pathway in Brassica rapa Is Enhanced under Heat Stress
3.4. ROS Metabolic Pathways Are Altered under Heat Stress in Brassica rapa
4. Material and Methods
4.1. Plant Cultivation and Heat Stress Treatment
4.2. Sample Collection and RNA Sequencing
4.3. Differential Expression Gene Analysis
4.4. Go and KEGG Pathway Analysis
4.5. Real Time Quantitative PCR
4.6. Statistical Analyses
4.7. Physiological Index Measurement
4.8. Metabolomic Analysis
4.9. Data Processing and Differential Metabolites Identification
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yu, J.; Li, P.; Tu, S.; Feng, N.; Chang, L.; Niu, Q. Integrated Analysis of the Transcriptome and Metabolome of Brassica rapa Revealed Regulatory Mechanism under Heat Stress. Int. J. Mol. Sci. 2023, 24, 13993. https://doi.org/10.3390/ijms241813993
Yu J, Li P, Tu S, Feng N, Chang L, Niu Q. Integrated Analysis of the Transcriptome and Metabolome of Brassica rapa Revealed Regulatory Mechanism under Heat Stress. International Journal of Molecular Sciences. 2023; 24(18):13993. https://doi.org/10.3390/ijms241813993
Chicago/Turabian StyleYu, Jing, Pengli Li, Song Tu, Ningxiao Feng, Liying Chang, and Qingliang Niu. 2023. "Integrated Analysis of the Transcriptome and Metabolome of Brassica rapa Revealed Regulatory Mechanism under Heat Stress" International Journal of Molecular Sciences 24, no. 18: 13993. https://doi.org/10.3390/ijms241813993