Identification of the Solid Stem Suppressor Gene Su-TdDof in Synthetic Hexaploid Wheat Syn-SAU-117
Abstract
:1. Introduction
2. Results
2.1. Differential Expression of the TdDof Gene in Different Materials
2.2. Genetic Analysis of the Solid Stem Suppressor Gene Su-TdDof
2.3. BSR-Seq Analysis of the RNA of Bulks with Contrasting Stem Solidity
2.4. Molecular Mapping of Su-TdDof
2.5. Gene Analysis of the Genomic Region of Su-TdDof
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Population Construction and Phenotypic Investigation
4.3. Observation of the Anatomical Structures of Stems
4.4. Solid Stem Gene Expression Analysis
4.5. Bulked Segregant RNA-Seq (BSR-Seq)
4.6. Kompetitive Allele-Specific PCR (KASP) Assays
4.7. Data Analysis
4.8. Candidate Gene Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parents and Cross | Generation a | No. of Plants/Families | Observed Ratio b | Actual Ratio | Expected Ratio | χ2 | p-Value | ||
---|---|---|---|---|---|---|---|---|---|
S | Seg | Ss | |||||||
Syn-SAU-117 | PSs | 20 | 20 | ||||||
Syn-SAU-119 | PS | 20 | 20 | ||||||
PSs × PS | F1 | 20 | 20 | ||||||
F2 | 156 | 36 | 120 | 0.9:3 | 1:3 | 0.308 | 0.579 | ||
F2:3 | 134 | 35 | 67 | 32 | 1.04:2:0.96 | 1:2:1 | 0.134 | 0.935 |
Marker | Physical Position (bp) | Allele 1 Primer a | Allele 2 Primer b | Common/Reverse Primer |
---|---|---|---|---|
KASP-533 | 5,336,907 | TCAGCTTCAATTTCGGCAGC | TCAGCTTCAATTTCGGCAGT | AGAAGCTGAACGTGCGGAAG |
KASP-669 | 6,695,986 | GTCGGATTCGGTTACTTTGAC | GTCGGATTCGGTTACTTTGAT | AGAGGTGCATGGTGTCGT |
KASP-1055 | 10,558,194 | TCTTTCTCCTTCAGCCTCTTA | TCTTTCTCCTTCAGCCTCTTG | GCCTGATTGTAGTACATTATG |
KASP-1166 | 11,664,145 | AACGAGGTCCCGCGCTCCTCCC | AACGAGGTCCCGCGCTCCTCCG | GTGTGAAGAGCGCTTCTGC |
Parents | Marker Genotype a | |||
---|---|---|---|---|
KASP-533 | KASP-669 | KASP-1055 | KASP-1166 | |
AS92 | CC | CC | AA | CC |
Syn-SAU-117 | CC | CC | AA | CC |
Syn-SAU-119 | TT | TT | GG | GG |
AS96 | TT | TT | GG | GG |
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Li, H.; Liu, X.; Zhang, J.; Chen, L.; Zhang, M.; Miao, Y.; Ma, P.; Hao, M.; Jiang, B.; Ning, S.; et al. Identification of the Solid Stem Suppressor Gene Su-TdDof in Synthetic Hexaploid Wheat Syn-SAU-117. Int. J. Mol. Sci. 2023, 24, 12845. https://doi.org/10.3390/ijms241612845
Li H, Liu X, Zhang J, Chen L, Zhang M, Miao Y, Ma P, Hao M, Jiang B, Ning S, et al. Identification of the Solid Stem Suppressor Gene Su-TdDof in Synthetic Hexaploid Wheat Syn-SAU-117. International Journal of Molecular Sciences. 2023; 24(16):12845. https://doi.org/10.3390/ijms241612845
Chicago/Turabian StyleLi, Hui, Xin Liu, Junqing Zhang, Longyu Chen, Minghu Zhang, Yongping Miao, Pan Ma, Ming Hao, Bo Jiang, Shunzong Ning, and et al. 2023. "Identification of the Solid Stem Suppressor Gene Su-TdDof in Synthetic Hexaploid Wheat Syn-SAU-117" International Journal of Molecular Sciences 24, no. 16: 12845. https://doi.org/10.3390/ijms241612845