Magnetic Hyperthermia Nanoarchitectonics via Iron Oxide Nanoparticles Stabilised by Oleic Acid: Anti-Tumour Efficiency and Safety Evaluation in Animals with Transplanted Carcinoma
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characteristics of Magnetic Nanoparticles
2.2. Acute Toxicity
2.3. Local Irritant Effect and Effect on Blood
2.3.1. Local Irritant Effect
2.3.2. Effect on Blood
2.4. Antineoplastic Effect of MHT
2.4.1. Study of Heating and Growth of W256 Adenocarcinoma
2.4.2. Tumour Growth
2.4.3. Blood Analysis
2.4.4. Histological Observation of Region of Treatment
2.4.5. Influence of HT on Survival Period of Rats
3. Materials and Methods
3.1. Reagents
3.2. Synthesis and Characterisation of Magnetic Nanoparticles
3.3. Animals
3.4. Acute (Single-Dose) Toxicity of Fe3O4-OA-NP
3.4.1. Intramuscular Administration
3.4.2. Intraperitoneal Administration
3.5. Study of Local Irritant Action
3.6. Antineoplastic Effect and Safety of MHT
3.6.1. Establishment of Animal Tumour Model
3.6.2. Experimental Groups
3.6.3. Induction of HT
3.6.4. Assessment of Tumour Growth and Rat Survival Time
3.7. Euthanasia
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Dose, mg Fe/kg | 95% Confidence Intervals Limits | |
---|---|---|---|
Lower | Upper | ||
LD16 | 183.8 | 20.6 | 347.4 |
LD50 | 652.3 | 343.2 | 1044.8 |
LD84 | 2315.5 | 1340.3 | 8488.8 |
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Kulikov, O.A.; Zharkov, M.N.; Ageev, V.P.; Yakobson, D.E.; Shlyapkina, V.I.; Zaborovskiy, A.V.; Inchina, V.I.; Balykova, L.A.; Tishin, A.M.; Sukhorukov, G.B.; et al. Magnetic Hyperthermia Nanoarchitectonics via Iron Oxide Nanoparticles Stabilised by Oleic Acid: Anti-Tumour Efficiency and Safety Evaluation in Animals with Transplanted Carcinoma. Int. J. Mol. Sci. 2022, 23, 4234. https://doi.org/10.3390/ijms23084234
Kulikov OA, Zharkov MN, Ageev VP, Yakobson DE, Shlyapkina VI, Zaborovskiy AV, Inchina VI, Balykova LA, Tishin AM, Sukhorukov GB, et al. Magnetic Hyperthermia Nanoarchitectonics via Iron Oxide Nanoparticles Stabilised by Oleic Acid: Anti-Tumour Efficiency and Safety Evaluation in Animals with Transplanted Carcinoma. International Journal of Molecular Sciences. 2022; 23(8):4234. https://doi.org/10.3390/ijms23084234
Chicago/Turabian StyleKulikov, Oleg A., Mikhail N. Zharkov, Valentin P. Ageev, Denis E. Yakobson, Vasilisa I. Shlyapkina, Andrey V. Zaborovskiy, Vera I. Inchina, Larisa A. Balykova, Alexander M. Tishin, Gleb B. Sukhorukov, and et al. 2022. "Magnetic Hyperthermia Nanoarchitectonics via Iron Oxide Nanoparticles Stabilised by Oleic Acid: Anti-Tumour Efficiency and Safety Evaluation in Animals with Transplanted Carcinoma" International Journal of Molecular Sciences 23, no. 8: 4234. https://doi.org/10.3390/ijms23084234