Mangiferin Ameliorates Obesity-Associated Inflammation and Autophagy in High-Fat-Diet-Fed Mice: In Silico and In Vivo Approaches
Abstract
:1. Introduction
2. Results
2.1. In Silico Network Pharmacology Approaches of MGF
2.1.1. Hub Genes of MGF and Insulin Resistance
2.1.2. Analysis of Functional Enrichment
2.1.3. Docking Simulation
2.2. In Vivo Experiments of the Effects of MGF on Glucose and Lipid Metabolism
2.2.1. Effects of MGF on Weight Gain
2.2.2. Effects of MGF on Glucose Metabolism
2.2.3. Effects of MGF on Lipid Metabolism
2.2.4. Effects of MGF on Adipose Tissue
2.2.5. Effects of MGF on Kupffer Cells (KCs) and Gene Expressions
2.2.6. Effects of MGF on Adipocyte and Hepatic Fat Area
3. Discussion
4. Materials and Methods
4.1. In Silico Research of the Effects of MGF on Insulin Resistance
4.1.1. Search for MGF Target Genes
4.1.2. Search for Overlapping Target Genes between MGF and Insulin Resistance
4.1.3. Analysis of Functional Enrichment
4.1.4. Docking Simulation
4.2. In Vivo Experiments to Explore the Effects of MGF on Insulin Resistance
4.2.1. Animals and Drug
4.2.2. Study Design and Drug Administration
4.2.3. Measuring BW, Oral Intake, and Liver and Fat Tissues
4.2.4. OGTT and Insulin Resistance Measurement
4.2.5. OFTT and Lipid Analysis
4.2.6. Analysis of Gene Expression in Adipose Tissue
4.2.7. Analysis of Gene Expression in Liver Tissue
4.2.8. Histological Analysis of the Epididymal Fat Pad and Liver
4.2.9. Isolation of Stromal Vascular Cells (SVCs) and Liver Immune Cells
4.2.10. Fluorescence-Activated Cell Sorting (FACS) of Macrophages
4.2.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Noh, J.-W.; Lee, H.-Y.; Lee, B.-C. Mangiferin Ameliorates Obesity-Associated Inflammation and Autophagy in High-Fat-Diet-Fed Mice: In Silico and In Vivo Approaches. Int. J. Mol. Sci. 2022, 23, 15329. https://doi.org/10.3390/ijms232315329
Noh J-W, Lee H-Y, Lee B-C. Mangiferin Ameliorates Obesity-Associated Inflammation and Autophagy in High-Fat-Diet-Fed Mice: In Silico and In Vivo Approaches. International Journal of Molecular Sciences. 2022; 23(23):15329. https://doi.org/10.3390/ijms232315329
Chicago/Turabian StyleNoh, Ji-Won, Han-Young Lee, and Byung-Cheol Lee. 2022. "Mangiferin Ameliorates Obesity-Associated Inflammation and Autophagy in High-Fat-Diet-Fed Mice: In Silico and In Vivo Approaches" International Journal of Molecular Sciences 23, no. 23: 15329. https://doi.org/10.3390/ijms232315329