Osteopontin Splicing Isoforms Contribute to Endometriotic Proliferation, Migration, and Epithelial-Mesenchymal Transition in Endometrial Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. Expression of OPN Isoforms, CD44 Variants, and EMT Markers in the Different Endometriotic Lesions
2.2. OPN Variants Regulated Epithelial-Mesenchymal Transaction by Inducing Actin Skeleton Remodeling
2.3. OPN Splice Variants Promoted Cell Proliferation and Migration by Binding to αvβ3 Integrin and CD44 Receptors in Endometriotic Cells
2.4. OPN Splice Variants Modulated the Migration of Endometriotic Epithelial Cells through the PI-3k or NF-ĸB Signaling Pathways
3. Discussion
4. Materials and Methods
4.1. Sample Collection
4.2. ELISA for OPN Levels in Body Fluids
4.3. Endometriosis Cell Culture
4.4. Cell Transfection
4.5. Scratch Wound Assay
4.6. Protein Extraction and SDS-PAGE Western Blotting
4.7. RNA Extraction and RT-PCR
4.8. siRNA Transfection
4.9. Laser Conjugate Microscopy
4.10. Bromodeoxyuridine Cell Proliferation Assay
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ho, N.-T.; Lin, S.-W.; Lee, Y.-R.; Tzeng, C.-R.; Kao, S.-H. Osteopontin Splicing Isoforms Contribute to Endometriotic Proliferation, Migration, and Epithelial-Mesenchymal Transition in Endometrial Epithelial Cells. Int. J. Mol. Sci. 2022, 23, 15328. https://doi.org/10.3390/ijms232315328
Ho N-T, Lin S-W, Lee Y-R, Tzeng C-R, Kao S-H. Osteopontin Splicing Isoforms Contribute to Endometriotic Proliferation, Migration, and Epithelial-Mesenchymal Transition in Endometrial Epithelial Cells. International Journal of Molecular Sciences. 2022; 23(23):15328. https://doi.org/10.3390/ijms232315328
Chicago/Turabian StyleHo, Nguyen-Tuong, Shu-Wei Lin, Yi-Rong Lee, Chii-Ruey Tzeng, and Shu-Huei Kao. 2022. "Osteopontin Splicing Isoforms Contribute to Endometriotic Proliferation, Migration, and Epithelial-Mesenchymal Transition in Endometrial Epithelial Cells" International Journal of Molecular Sciences 23, no. 23: 15328. https://doi.org/10.3390/ijms232315328