Plasma Neurofilament Light Chain (NF-L) Is a Prognostic Biomarker for Cortical Damage Evolution but Not for Cognitive Impairment or Epileptogenesis Following Experimental TBI
Abstract
:1. Introduction
2. Results
2.1. Sample Quality and Lack of a Hemolysis Effect on Plasma NF-L Levels
2.2. Post-TBI Increase in Plasma NF-L Levels Is Time-Dependent
2.3. Plasma NF-L Levels Correlated with the Severity of Acute and Chronic Cortical Damage
2.3.1. Plasma NF-L and Volume of Abnormal Cortical T2 in MRI
2.3.2. Plasma NF-L and Cortical Lesion Area in Histologic Sections
2.4. Plasma NF-L as a Diagnostic Biomarker for Sham-Operation and TBI
2.5. Plasma NF-L as a Prognostic Biomarker for Somatomotor Recovery
2.6. Plasma NF-L as a Prognostic Biomarker for Memory Impairment
2.7. Plasma NF-L as a Prognostic Biomarker for Post-Traumatic Epileptogenesis
3. Discussion
3.1. Presence and Temporal Profile of NF-L in the Plasma after TBI
3.2. Acute Plasma NF-L Levels Report on Cortical Lesion Severity in Structural MRI and Histology
3.3. Elevated Plasma NF-L as a Diagnostic Biomarker for TBI and Craniotomy in the Rat Lateral FPI Model
3.4. Increased Plasma NF-L Levels Correlate with Acute Somatomotor Impairment but Not with Recovery after TBI
3.5. Increased Plasma NF-L Levels Do Not Differentiate Animals with or without Chronic Hippocampus-Dependent Memory Impairment after TBI
3.6. Elevated Plasma NF-L Levels Do Not Predict Post-Traumatic Epileptogenesis
3.7. Methodologic Considerations
4. Materials and Methods
4.1. Animals
4.2. Lateral Fluid-Percussion-Induced Traumatic Brain Injury
4.3. Analysis of Plasma NF-L Levels
4.4. Behavioral Tests
4.4.1. Composite Neuromotor Score (Neuroscore)
4.4.2. Morris Water Maze
4.5. Magnetic Resonance Imaging (MRI) and Lesion Analysis
4.6. Video-EEG Monitoring
4.7. Histology and Preparation of Cortical Unfolded Maps
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Heiskanen, M.; Jääskeläinen, O.; Manninen, E.; Das Gupta, S.; Andrade, P.; Ciszek, R.; Gröhn, O.; Herukka, S.-K.; Puhakka, N.; Pitkänen, A. Plasma Neurofilament Light Chain (NF-L) Is a Prognostic Biomarker for Cortical Damage Evolution but Not for Cognitive Impairment or Epileptogenesis Following Experimental TBI. Int. J. Mol. Sci. 2022, 23, 15208. https://doi.org/10.3390/ijms232315208
Heiskanen M, Jääskeläinen O, Manninen E, Das Gupta S, Andrade P, Ciszek R, Gröhn O, Herukka S-K, Puhakka N, Pitkänen A. Plasma Neurofilament Light Chain (NF-L) Is a Prognostic Biomarker for Cortical Damage Evolution but Not for Cognitive Impairment or Epileptogenesis Following Experimental TBI. International Journal of Molecular Sciences. 2022; 23(23):15208. https://doi.org/10.3390/ijms232315208
Chicago/Turabian StyleHeiskanen, Mette, Olli Jääskeläinen, Eppu Manninen, Shalini Das Gupta, Pedro Andrade, Robert Ciszek, Olli Gröhn, Sanna-Kaisa Herukka, Noora Puhakka, and Asla Pitkänen. 2022. "Plasma Neurofilament Light Chain (NF-L) Is a Prognostic Biomarker for Cortical Damage Evolution but Not for Cognitive Impairment or Epileptogenesis Following Experimental TBI" International Journal of Molecular Sciences 23, no. 23: 15208. https://doi.org/10.3390/ijms232315208