eDNA, Amyloid Fibers and Membrane Vesicles Identified in Pseudomonas fluorescens SBW25 Biofilms
Abstract
:1. Introduction
2. Results
2.1. Visualization of eDNA in Viscous Mass and Wrinkly Spreader Biofilms
2.2. Role of eDNA in Biofilm Strength and Attachment Levels
2.3. Viscous Mass and Wrinkly Spreader Biofilms include Amyloid Fibers
2.4. Biofilm Membrane Vesicles and Matrix-Associated Proteins
3. Discussion
4. Materials and Methods
4.1. Bacteria and Culturing Conditions
4.2. Combined Biofilm Assay
4.3. Spectrophometric Measurement of eDNA
4.4. Confocal Laser Scanning Microscopy (CLSM) and Transmission Electron Microscopy (TEM)
4.5. Membrane Vesicle (MV) Preparation
4.6. Matrix-Associated Proteins
4.7. MALDI-TOF Mass Spectometry and Protein Identification
4.8. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Moshynets, O.V.; Pokholenko, I.; Iungin, O.; Potters, G.; Spiers, A.J. eDNA, Amyloid Fibers and Membrane Vesicles Identified in Pseudomonas fluorescens SBW25 Biofilms. Int. J. Mol. Sci. 2022, 23, 15096. https://doi.org/10.3390/ijms232315096
Moshynets OV, Pokholenko I, Iungin O, Potters G, Spiers AJ. eDNA, Amyloid Fibers and Membrane Vesicles Identified in Pseudomonas fluorescens SBW25 Biofilms. International Journal of Molecular Sciences. 2022; 23(23):15096. https://doi.org/10.3390/ijms232315096
Chicago/Turabian StyleMoshynets, Olena V., Ianina Pokholenko, Olga Iungin, Geert Potters, and Andrew J. Spiers. 2022. "eDNA, Amyloid Fibers and Membrane Vesicles Identified in Pseudomonas fluorescens SBW25 Biofilms" International Journal of Molecular Sciences 23, no. 23: 15096. https://doi.org/10.3390/ijms232315096